##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547059_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1108035
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6353093539464	31.0	31.0	33.0	30.0	34.0
2	32.02120871633116	33.0	31.0	34.0	30.0	34.0
3	32.02196681512768	33.0	31.0	34.0	30.0	34.0
4	35.7544978272347	37.0	35.0	37.0	35.0	37.0
5	35.71736181618812	37.0	35.0	37.0	33.0	37.0
6	35.77935624777196	37.0	35.0	37.0	33.0	37.0
7	36.112595721254294	37.0	35.0	37.0	35.0	37.0
8	36.05638901298244	37.0	35.0	37.0	35.0	37.0
9	37.823832279666256	39.0	38.0	39.0	35.0	39.0
10	37.38799225656229	39.0	37.0	39.0	34.0	39.0
11	37.10568619222317	39.0	37.0	39.0	33.0	39.0
12	36.56890260686711	39.0	35.0	39.0	33.0	39.0
13	36.35862405068432	39.0	35.0	39.0	32.0	39.0
14	37.45889525150379	40.0	35.0	41.0	32.0	41.0
15	37.62788540073193	40.0	35.0	41.0	33.0	41.0
16	37.72424788025649	40.0	35.0	41.0	33.0	41.0
17	37.68663895996065	40.0	35.0	41.0	33.0	41.0
18	37.52964662668598	39.0	36.0	41.0	33.0	41.0
19	37.40959446226879	39.0	35.0	41.0	33.0	41.0
20	37.18585604245353	39.0	35.0	41.0	32.0	41.0
21	37.127308252898146	39.0	35.0	41.0	32.0	41.0
22	37.043337078702386	39.0	35.0	41.0	32.0	41.0
23	36.98738216753081	38.0	35.0	41.0	32.0	41.0
24	36.900508557942665	38.0	35.0	41.0	32.0	41.0
25	36.79179448302626	38.0	35.0	41.0	32.0	41.0
26	36.67620517402429	38.0	35.0	41.0	32.0	41.0
27	36.56989806278683	38.0	35.0	41.0	31.0	41.0
28	36.58098345268877	38.0	35.0	41.0	31.0	41.0
29	36.5713691354515	38.0	35.0	41.0	31.0	41.0
30	36.47877819743961	38.0	35.0	41.0	31.0	41.0
31	36.306095926572716	38.0	35.0	41.0	31.0	41.0
32	36.068086296913	38.0	35.0	41.0	30.0	41.0
33	35.82861191207859	38.0	35.0	41.0	29.0	41.0
34	35.578523241594354	38.0	35.0	41.0	27.0	41.0
35	35.41986579846304	38.0	35.0	41.0	27.0	41.0
36	35.27726921983511	38.0	35.0	41.0	25.0	41.0
37	35.20462079266449	38.0	35.0	41.0	25.0	41.0
38	35.12668101639389	38.0	34.0	41.0	25.0	41.0
39	35.048494858014415	38.0	34.0	40.0	24.0	41.0
40	34.95044651116616	38.0	34.0	40.0	24.0	41.0
41	34.90955971607395	38.0	34.0	40.0	24.0	41.0
42	34.80952587237768	37.0	34.0	40.0	23.0	41.0
43	34.68384753189204	37.0	34.0	40.0	23.0	41.0
44	34.54136647308072	37.0	34.0	40.0	23.0	41.0
45	34.46277509284454	37.0	34.0	40.0	23.0	41.0
46	34.422769136354	37.0	34.0	40.0	23.0	41.0
47	34.366778125239726	36.0	34.0	40.0	23.0	41.0
48	34.28362280974879	36.0	34.0	40.0	23.0	41.0
49	34.21160883907097	36.0	34.0	40.0	23.0	41.0
50	34.11579327367818	36.0	34.0	40.0	23.0	41.0
51	33.7396733857685	35.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	4.0
13	4.0
14	9.0
15	35.0
16	97.0
17	209.0
18	456.0
19	898.0
20	1571.0
21	2495.0
22	3919.0
23	5921.0
24	9082.0
25	14164.0
26	20235.0
27	23197.0
28	23104.0
29	23170.0
30	25298.0
31	29083.0
32	35687.0
33	45822.0
34	81933.0
35	137171.0
36	73839.0
37	96752.0
38	147496.0
39	306127.0
40	253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.15124070990537	23.36451465883298	27.02477809816477	17.45946653309688
2	34.60197556936379	24.065304796328636	28.237916672307286	13.0948029620003
3	28.21030021614841	23.85484213043812	34.73184511319588	13.203012540217593
4	25.389901943530663	26.106215056383597	34.30198504559874	14.201897954486997
5	23.191325183771273	30.483784356992334	32.3362529161985	13.988637543037902
6	20.88192159994946	39.19984477024643	29.12146276967785	10.796770860126259
7	83.89942555966192	3.4732657361906436	10.7326032119924	1.8947054921550313
8	84.47287314931387	2.7768075918179482	10.430446691665878	2.3198725672022995
9	79.95749231748096	4.567996498305559	12.377226351153167	3.0972848330603275
10	44.590649212344374	26.786969725685562	17.52832717378061	11.094053888189451
11	36.091820204235425	21.90463297639515	27.256629980099905	14.746916839269517
12	31.938973046880285	20.883907096797483	30.260867210873304	16.916252645448925
13	22.592697884092107	29.583632285983747	30.789460621731262	17.034209208192884
14	17.24413037494303	31.934731303614054	32.85212109725776	17.96901722418516
15	17.071211649451506	23.444295532180842	42.976620774614524	16.50787204375313
16	19.853975731813524	21.798138145455695	39.869227957600614	18.478658165130163
17	20.64312047904624	21.25077276439824	31.310653544337498	26.795453212218025
18	22.625187832514314	23.002161484068644	34.40496013212579	19.967690551291252
19	25.80857102889349	24.773495422076017	29.63868469858804	19.77924885044245
20	26.96178369816838	24.75643819915436	28.479154539342165	19.802623563335093
21	23.64284521698322	26.05702888446665	30.674572554116068	19.625553344434067
22	22.812456285225647	22.99701724223513	30.647768346667746	23.542758125871472
23	20.60088354609737	26.42651179791252	30.50093182977072	22.47167282621939
24	20.90818430825741	23.597991038189225	35.69661608162197	19.797208571931392
25	20.042507682519055	25.24748766961332	33.47367186054592	21.2363327873217
26	19.627809590852273	28.913436849918998	30.01710234785002	21.4416512113787
27	20.020035468193694	27.31177264256093	31.745657853768154	20.92253403547722
28	18.792817916401557	25.753247866719008	36.01267107988466	19.44126313699477
29	19.498662045874003	24.22170779803887	34.66181122437468	21.617818931712446
30	21.20339158961585	25.51561999395326	32.26820452422532	21.012783892205572
31	26.436619781866096	24.475039145875353	28.152269558272074	20.93607151398647
32	26.650782691882473	25.146768829504484	28.153803805836457	20.048644672776582
33	25.595310617444394	26.03545916870857	27.848939789808085	20.52029042403895
34	21.40988326181032	26.836155897602517	29.74382578167657	22.010135058910592
35	21.563849517388892	26.89815754917489	30.004286868194598	21.53370606524162
36	25.851890960123104	26.76675375777841	27.19183058296895	20.189524699129542
37	22.403804933959666	29.624064221798047	27.508968579512377	20.463162264729906
38	21.891636997026268	30.554449994810636	25.90008438361604	21.65382862454706
39	21.267198238322795	29.562062570225674	27.2301867720785	21.940552419373034
40	22.244965186117767	26.755472525687367	27.5472345187652	23.452327769429665
41	19.822478531815328	25.995839481604822	28.207051221306184	25.97463076527366
42	22.26409815574418	26.948155969802396	26.613148501626753	24.17459737282667
43	22.228539712193207	26.74599629073089	27.661942086666937	23.363521910408966
44	21.27577197471199	28.208134219586928	28.552347173148863	21.96374663255222
45	20.504677198824947	29.589949775954732	27.000230137134658	22.905142888085663
46	22.93763283650787	28.592959608676622	27.67159882133687	20.797808733478636
47	22.3905382050206	26.30106449706011	28.791148294052082	22.517249003867207
48	22.76480436087308	24.472151150460046	29.6605251639163	23.102519324750574
49	21.655092122541255	24.220985799185044	30.478098616018446	23.64582346225525
50	19.7421561593271	27.916175933070708	29.197633648756582	23.144034258845615
51	19.699016727810946	29.28761275591475	27.306989400154325	23.706381116119978
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	606.0
1	1044.5
2	1483.0
3	11660.5
4	21838.0
5	15063.0
6	8288.0
7	8168.0
8	8048.0
9	8231.5
10	8415.0
11	8397.5
12	8380.0
13	8038.0
14	7696.0
15	7210.5
16	6725.0
17	6479.5
18	6234.0
19	5873.5
20	5513.0
21	5392.5
22	5272.0
23	5404.5
24	5537.0
25	6254.0
26	7756.0
27	8541.0
28	10569.0
29	12597.0
30	13910.0
31	15223.0
32	17550.5
33	19878.0
34	23158.0
35	26438.0
36	28902.0
37	31366.0
38	35764.0
39	40162.0
40	49518.0
41	58874.0
42	68317.0
43	77760.0
44	82400.5
45	87041.0
46	87646.0
47	88251.0
48	89127.0
49	90003.0
50	85775.0
51	81547.0
52	75454.0
53	69361.0
54	64435.5
55	59510.0
56	55454.5
57	51399.0
58	48057.5
59	44716.0
60	42078.5
61	39441.0
62	36250.5
63	33060.0
64	28447.5
65	23835.0
66	20443.0
67	17051.0
68	14536.5
69	12022.0
70	9873.5
71	7725.0
72	6463.0
73	5201.0
74	4047.5
75	2312.0
76	1730.0
77	1225.5
78	721.0
79	590.0
80	459.0
81	288.0
82	117.0
83	88.5
84	60.0
85	44.0
86	28.0
87	18.5
88	9.0
89	7.5
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1108035.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.49425764409597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75359149210848	22.15530868729928
2	6.384746866570487	3.638572444167878
3	2.4423696523300658	2.08780530406842
4	1.4784572272287089	1.6851016459372228
5	1.036342346347989	1.476490291216327
6	0.8134016114637589	1.3906365051102714
7	0.7089349637896609	1.4140402857791314
8	0.57765915479445	1.3167975029747017
9	0.5035962378148694	1.2914640854004884
>10	7.843434387449409	53.4522315578313
>50	0.4293732904012494	7.3670909077746565
>100	0.02649658960427451	1.259941447619101
>500	0.0012769440773085507	0.2417821361462698
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.192360193271377E-4	1.2227371986750033
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13442	1.2131385741425136	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04674942578528657	0.0
2	0.0	0.0	0.0	0.14918301317196658	0.0
3	0.0	0.0	0.0	0.24954085385389452	0.0
4	0.0	0.0	0.0	0.390240380493396	0.0
5	0.0	0.0	0.0	0.7423953214474271	0.0
6	0.0	0.0	0.0	1.3013126841661138	0.0
7	0.0	0.0	0.0	1.684152576407785	0.0
8	0.0	0.0	0.0	2.5573199402545947	0.0
9	0.0	0.0	0.0	3.0585676445238645	0.0
10	0.0	0.0	0.0	3.67560591497561	0.0
11	0.0	0.0	0.0	4.081640020396468	0.0
12	0.0	0.0	0.0	4.436863456479263	0.0
13	0.0	0.0	0.0	4.632615395723059	0.0
14	0.0	0.0	0.0	4.725843497723448	0.0
15	0.0	0.0	0.0	4.820605847288218	0.0
16	0.0	0.0	0.0	4.980528593410858	0.0
17	0.0	0.0	0.0	5.171316790534595	0.0
18	0.0	0.0	0.0	5.43944911487453	0.0
19	0.0	0.0	0.0	5.575906898247799	0.0
20	0.0	0.0	0.0	5.731226901677293	0.0
21	0.0	0.0	0.0	5.919668602526094	0.0
22	0.0	0.0	0.0	6.113435044921866	0.0
23	0.0	0.0	0.0	6.328319953792073	0.0
24	0.0	0.0	0.0	6.505390172693101	0.0
25	0.0	0.0	0.0	6.656829432283276	0.0
26	0.0	0.0	0.0	6.799875455197715	0.0
27	0.0	0.0	0.0	6.949780467223508	0.0
28	0.0	0.0	0.0	7.109432463775963	0.0
29	0.0	0.0	0.0	7.291827424223964	0.0
30	0.0	0.0	0.0	7.5420902769316855	0.0
31	1.8049971345670488E-4	0.0	0.0	7.738203215602395	0.0
32	1.8049971345670488E-4	0.0	0.0	7.921861674044592	0.0
33	1.8049971345670488E-4	0.0	0.0	8.101458888934014	0.0
34	1.8049971345670488E-4	0.0	0.0	8.291795836774108	0.0
35	1.8049971345670488E-4	0.0	0.0	8.506861245357774	0.0
36	1.8049971345670488E-4	0.0	0.0	8.704598681449593	0.0
37	1.8049971345670488E-4	0.0	0.0	8.897191875707897	0.0
38	1.8049971345670488E-4	0.0	0.0	9.123719016096063	0.0
39	1.8049971345670488E-4	0.0	0.0	9.360805389721444	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	20	7.033176E-4	45.000004	1
CGTTTAG	20	7.033176E-4	45.000004	31
TAGCGCG	25	3.8907143E-5	45.0	1
AGTCGAC	25	3.8907143E-5	45.0	35
TATAACG	30	2.1654305E-6	44.999996	1
CGTTTTT	7980	0.0	43.843983	1
TACGGGT	70	0.0	41.785713	4
GCGTAAG	60	3.6379788E-12	41.249996	1
AACGTTG	45	1.9281288E-8	40.0	1
ACGGGAT	365	0.0	39.452057	5
TACGGGA	325	0.0	38.76923	4
TCGACAA	140	0.0	38.571426	19
CTACGGG	170	0.0	38.38235	3
TAAGGGA	1105	0.0	38.076923	4
AACACGT	65	9.094947E-12	38.07692	41
TTACGGG	320	0.0	37.968754	3
AGGGCGA	840	0.0	37.76786	6
TAAGGGT	305	0.0	37.622948	4
GGGCGAT	1330	0.0	37.556393	7
AACGTAG	30	1.1398955E-4	37.499996	1
>>END_MODULE