FastQCFastQC Report
Thu 26 May 2016
SRR1547058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547058_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences761856
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52050.6831999747983871No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG502.1827873E-1145.01
TTAGACG253.889563E-545.01
TACGGGT406.8102963E-945.04
TAACGCC253.889563E-545.012
CGTTACC207.031788E-445.035
GTGTCGA253.889563E-545.043
TATTACG207.031788E-445.01
CGGTCTA406.8102963E-945.031
TGTACGT207.031788E-445.038
TAATGCG502.1827873E-1145.01
AACACGT207.031788E-445.041
AATGCGG1600.042.18752
CGTTTTT28900.042.1193771
GCGATCA1000.040.59
AGTAGCG501.0804797E-940.51
CGTTAGG1900.040.2631572
CTCACGA451.9268555E-840.00000424
TCAACGG451.9268555E-840.0000042
TAAGGGA6050.038.677694
ACGTAAG356.2455165E-638.5714261