##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547058_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	761856
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.635613554267472	31.0	31.0	33.0	30.0	34.0
2	32.02104990969422	33.0	31.0	34.0	30.0	34.0
3	32.10753213205645	33.0	31.0	34.0	30.0	34.0
4	35.790054813508064	37.0	35.0	37.0	35.0	37.0
5	35.729408182123656	37.0	35.0	37.0	33.0	37.0
6	35.780203870547716	37.0	35.0	37.0	35.0	37.0
7	36.089507728494624	37.0	35.0	37.0	35.0	37.0
8	36.02375252016129	37.0	35.0	37.0	35.0	37.0
9	37.793470157090056	39.0	38.0	39.0	35.0	39.0
10	37.37944178427419	39.0	37.0	39.0	34.0	39.0
11	37.08662660660282	39.0	37.0	39.0	33.0	39.0
12	36.37444740213374	38.0	35.0	39.0	33.0	39.0
13	36.07127068632393	38.0	35.0	39.0	32.0	39.0
14	37.051849693380376	39.0	35.0	41.0	32.0	41.0
15	37.296708301831316	39.0	35.0	41.0	32.0	41.0
16	37.439139417422716	39.0	35.0	41.0	33.0	41.0
17	37.4158686155914	39.0	35.0	41.0	33.0	41.0
18	37.31086189516129	39.0	35.0	41.0	33.0	41.0
19	37.24939621135753	39.0	35.0	41.0	32.0	41.0
20	37.05044391591062	39.0	35.0	41.0	32.0	41.0
21	36.9686528687836	38.0	35.0	41.0	32.0	41.0
22	36.897559381300404	38.0	35.0	41.0	32.0	41.0
23	36.84761949764785	38.0	35.0	41.0	32.0	41.0
24	36.75836509786626	38.0	35.0	41.0	32.0	41.0
25	36.63680406796035	38.0	35.0	41.0	31.0	41.0
26	36.505982757896504	38.0	35.0	41.0	31.0	41.0
27	36.399139732442876	38.0	35.0	40.0	31.0	41.0
28	36.41419113323253	38.0	35.0	40.0	31.0	41.0
29	36.43772576444893	38.0	35.0	41.0	31.0	41.0
30	36.40889879452285	38.0	35.0	40.0	31.0	41.0
31	36.252192015288976	38.0	35.0	40.0	31.0	41.0
32	36.130907940608196	38.0	35.0	41.0	30.0	41.0
33	36.01740617649529	38.0	35.0	41.0	30.0	41.0
34	35.851834204889116	38.0	35.0	41.0	30.0	41.0
35	35.749614100302416	38.0	35.0	41.0	29.0	41.0
36	35.63077405703965	38.0	35.0	41.0	29.0	41.0
37	35.555106214297716	38.0	35.0	40.0	29.0	41.0
38	35.477146599882396	37.0	35.0	40.0	29.0	41.0
39	35.391381048387096	37.0	35.0	40.0	28.0	41.0
40	35.26931861139113	37.0	34.0	40.0	27.0	41.0
41	35.227012716313844	37.0	34.0	40.0	27.0	41.0
42	35.128071446572584	37.0	34.0	40.0	27.0	41.0
43	35.00968293220766	36.0	34.0	40.0	27.0	41.0
44	34.88238065986223	36.0	34.0	40.0	27.0	41.0
45	34.79492581275202	36.0	34.0	40.0	26.0	41.0
46	34.76581269951277	36.0	34.0	40.0	26.0	41.0
47	34.717695995043684	36.0	34.0	40.0	26.0	41.0
48	34.63194750714046	35.0	34.0	40.0	26.0	41.0
49	34.56497290826613	36.0	34.0	40.0	26.0	41.0
50	34.44473890078965	35.0	34.0	40.0	26.0	41.0
51	34.06743925361223	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	2.0
14	8.0
15	26.0
16	58.0
17	143.0
18	318.0
19	606.0
20	1123.0
21	1704.0
22	2517.0
23	3827.0
24	5451.0
25	7786.0
26	10495.0
27	12633.0
28	13512.0
29	14745.0
30	17464.0
31	20680.0
32	25745.0
33	33996.0
34	63376.0
35	107933.0
36	52482.0
37	67982.0
38	101192.0
39	195878.0
40	166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.00385899697581	22.294764364919356	27.993347824260752	14.708028813844088
2	32.27355300739247	24.505812121975808	29.50439453125	13.716240339381722
3	29.87729964717742	24.505680863575268	31.353431619623656	14.263587869623656
4	26.79994644657258	26.876995127688176	31.0934087281586	15.229649697580644
5	24.241195186491936	31.212985131048388	29.397550193212364	15.148269489247312
6	22.449649277553764	40.369046118951616	26.43373550907258	10.747569094422044
7	85.96165154569893	4.207356770833334	7.612593455981183	2.218398227486559
8	86.63238197244624	3.614462575604839	7.301248529905914	2.451906922043011
9	82.66142158098118	4.696950604838709	8.904307375672044	3.737320438508065
10	50.13283350134409	24.072396883400536	13.798801873319894	11.995967741935484
11	42.107826150873656	20.778336063508064	21.731009534610216	15.382828251008066
12	35.84706296202957	20.946084299395164	26.342642179099464	16.864210559475808
13	24.754546790994624	30.737829721102152	27.133999075940864	17.373624411962364
14	18.179025957661292	34.09371324764785	30.17131846438172	17.55594233030914
15	17.40893292170699	22.190938970094088	43.05656186995967	17.343566238239248
16	20.660466019825268	20.128869497647848	39.477670320900536	19.732994161626344
17	22.125309769825268	19.827237693212364	28.168052755376344	29.87939978158602
18	24.336751302083336	22.413421959005376	31.709535660282256	21.540291078629032
19	29.236233618951612	23.470314600134408	26.063980594758064	21.229471186155912
20	30.04701675907258	24.154039608534948	24.8911867859543	20.907756846438172
21	25.840316280241936	25.438665574596776	28.24864541330645	20.472372731854836
22	24.70873760920699	22.491126932123656	27.941369497647848	24.858765961021508
23	22.712034820228492	26.167805989583332	27.28665259576613	23.833506594422044
24	22.794727612567204	23.392478368615592	33.274135794690864	20.538658224126344
25	21.323058425739248	24.517756636424732	31.32980510752688	22.82937983030914
26	20.741714969758064	29.09499957997312	27.18505859375	22.97822685651882
27	21.120264196908604	27.337449596774192	29.809439054099464	21.73284715221774
28	19.637044270833336	25.282074302755376	34.237572454637096	20.843308971774192
29	19.683115969422044	24.354602444556452	33.878843245967744	22.083438340053764
30	22.895009030577956	24.945921538978492	30.29023857526882	21.868830855174732
31	28.033381636424732	23.525049353158604	26.07146232358871	22.370106686827956
32	29.03383316532258	24.993830855174732	25.77455582157258	20.197780157930108
33	26.996046496975808	24.64258337533602	26.62629158266129	21.73507854502688
34	22.229528939852152	25.428427419354836	29.072029359879032	23.27001428091398
35	22.441642515120968	25.630040322580644	29.76835517473118	22.159961987567204
36	28.001223328293012	24.36287172379032	26.59912109375	21.036783854166664
37	23.812767767137096	27.741331695228492	27.365013860887093	21.080886676747312
38	23.456663726478492	29.607169858870968	24.490848664314516	22.44531775033602
39	23.398122479838708	27.49299080141129	26.376113071236556	22.73277364751344
40	24.723963583669356	24.853253108198924	26.080650411626344	24.342132896505376
41	21.277774277553764	23.719049269153224	27.037655409946236	27.965521043346776
42	23.67494644657258	25.882056451612907	25.277611517137093	25.16538558467742
43	23.686759702620968	24.98228011592742	26.052692372311824	25.278267809139788
44	22.26116221438172	26.898521505376344	27.609942036290324	23.230374243951612
45	20.745652721774192	29.506363407258064	26.005964381720432	23.742019489247312
46	24.095104586693548	27.435237105174732	27.052618867607524	21.417039440524192
47	23.456794984879032	25.498256888440864	28.071971606182792	22.972976520497312
48	23.949539020497312	23.599210349462364	29.0537844422043	23.39746618783602
49	22.596527427755376	22.45214318716398	30.620222194220432	24.331107190860216
50	20.869035618279568	28.124606224798388	27.777033980174732	23.229324176747312
51	20.94162151377688	29.976399739583332	25.244796917002688	23.837181829637096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	375.0
1	717.0
2	1059.0
3	4781.0
4	8503.0
5	5858.5
6	3214.0
7	3207.0
8	3200.0
9	3312.5
10	3425.0
11	3414.5
12	3404.0
13	3216.5
14	3029.0
15	2896.5
16	2764.0
17	2702.0
18	2640.0
19	2548.0
20	2456.0
21	2523.5
22	2591.0
23	2921.5
24	3252.0
25	3534.5
26	4375.5
27	4934.0
28	6003.5
29	7073.0
30	8224.5
31	9376.0
32	10707.0
33	12038.0
34	13661.5
35	15285.0
36	16831.0
37	18377.0
38	21226.5
39	24076.0
40	31646.5
41	39217.0
42	46502.5
43	53788.0
44	56354.0
45	58920.0
46	58641.0
47	58362.0
48	57945.5
49	57529.0
50	56767.0
51	56005.0
52	52309.0
53	48613.0
54	45892.0
55	43171.0
56	41266.0
57	39361.0
58	38775.5
59	38190.0
60	35862.0
61	33534.0
62	31173.5
63	28813.0
64	25805.0
65	22797.0
66	19777.0
67	16757.0
68	14489.5
69	12222.0
70	10180.5
71	8139.0
72	6599.0
73	5059.0
74	4139.0
75	2479.5
76	1740.0
77	1321.5
78	903.0
79	638.5
80	374.0
81	265.5
82	157.0
83	114.0
84	71.0
85	43.0
86	15.0
87	10.5
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	761856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.264937405162165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9209553071758	19.434031209810733
2	5.644947632974787	2.8523849720505265
3	2.09672889817867	1.5892117310423615
4	1.2579595898747242	1.271290811856335
5	0.93980712334252	1.1872084072087146
6	0.790817522456201	1.1987973122256805
7	0.6401218398910499	1.1320846751567228
8	0.5754159111510624	1.1630277581732755
9	0.5299001465558236	1.2049104630347227
>10	10.216343532469327	61.69344775349261
>50	0.3624226076750505	5.519319590388477
>100	0.023533935563307105	0.9759095684812197
>500	5.22976345851269E-4	0.09064093744626547
>1k	0.0	0.0
>5k	5.22976345851269E-4	0.6877348096323787
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5205	0.6831999747983871	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.312584005376344E-4	0.0	0.0	0.03714612735215054	0.0
2	1.312584005376344E-4	0.0	0.0	0.13545866935483872	0.0
3	1.312584005376344E-4	0.0	0.0	0.20686323924731181	0.0
4	1.312584005376344E-4	0.0	0.0	0.3165952620967742	0.0
5	1.312584005376344E-4	0.0	0.0	0.6012947328629032	0.0
6	1.312584005376344E-4	0.0	0.0	0.9450604838709677	0.0
7	1.312584005376344E-4	0.0	0.0	1.1767315608198925	0.0
8	1.312584005376344E-4	0.0	0.0	1.7343172463037635	0.0
9	1.312584005376344E-4	0.0	0.0	2.031748781922043	0.0
10	1.312584005376344E-4	0.0	0.0	2.4667391213037635	0.0
11	1.312584005376344E-4	0.0	0.0	2.782546832997312	0.0
12	1.312584005376344E-4	0.0	0.0	3.0992733534946235	0.0
13	1.312584005376344E-4	0.0	0.0	3.231975596438172	0.0
14	1.312584005376344E-4	0.0	0.0	3.286841607862903	0.0
15	1.312584005376344E-4	0.0	0.0	3.35693359375	0.0
16	1.312584005376344E-4	0.0	0.0	3.502367901545699	0.0
17	1.312584005376344E-4	0.0	0.0	3.658565398185484	0.0
18	1.312584005376344E-4	0.0	0.0	3.8885301159274195	0.0
19	1.312584005376344E-4	0.0	0.0	4.003643733198925	0.0
20	1.312584005376344E-4	0.0	0.0	4.131489415322581	0.0
21	1.312584005376344E-4	0.0	0.0	4.281780283938172	0.0
22	1.312584005376344E-4	0.0	0.0	4.438109038978495	0.0
23	1.312584005376344E-4	0.0	0.0	4.609663768481183	0.0
24	1.312584005376344E-4	0.0	0.0	4.744203629032258	0.0
25	1.312584005376344E-4	0.0	0.0	4.855773269489247	0.0
26	1.312584005376344E-4	0.0	0.0	4.968918010752688	0.0
27	2.625168010752688E-4	0.0	0.0	5.088363155241935	0.0
28	2.625168010752688E-4	0.0	0.0	5.213058635752688	0.0
29	2.625168010752688E-4	0.0	0.0	5.345498361895161	0.0
30	2.625168010752688E-4	0.0	0.0	5.523747269825269	0.0
31	2.625168010752688E-4	0.0	0.0	5.667606476814516	0.0
32	2.625168010752688E-4	0.0	0.0	5.807396673387097	0.0
33	2.625168010752688E-4	0.0	0.0	5.950205813172043	0.0
34	2.625168010752688E-4	0.0	0.0	6.103121849798387	0.0
35	2.625168010752688E-4	0.0	0.0	6.281108240927419	0.0
36	2.625168010752688E-4	0.0	0.0	6.43034904233871	0.0
37	2.625168010752688E-4	0.0	0.0	6.591665616599462	0.0
38	2.625168010752688E-4	0.0	0.0	6.787240633400538	0.0
39	2.625168010752688E-4	0.0	0.0	7.082178259408602	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	50	2.1827873E-11	45.0	1
TTAGACG	25	3.889563E-5	45.0	1
TACGGGT	40	6.8102963E-9	45.0	4
TAACGCC	25	3.889563E-5	45.0	12
CGTTACC	20	7.031788E-4	45.0	35
GTGTCGA	25	3.889563E-5	45.0	43
TATTACG	20	7.031788E-4	45.0	1
CGGTCTA	40	6.8102963E-9	45.0	31
TGTACGT	20	7.031788E-4	45.0	38
TAATGCG	50	2.1827873E-11	45.0	1
AACACGT	20	7.031788E-4	45.0	41
AATGCGG	160	0.0	42.1875	2
CGTTTTT	2890	0.0	42.119377	1
GCGATCA	100	0.0	40.5	9
AGTAGCG	50	1.0804797E-9	40.5	1
CGTTAGG	190	0.0	40.263157	2
CTCACGA	45	1.9268555E-8	40.000004	24
TCAACGG	45	1.9268555E-8	40.000004	2
TAAGGGA	605	0.0	38.67769	4
ACGTAAG	35	6.2455165E-6	38.571426	1
>>END_MODULE