FastQCFastQC Report
Thu 26 May 2016
SRR1547057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547057_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1014147
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57170.5637249826701652No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA253.8904796E-545.014
TCTAGCG453.8380676E-1045.01
GCGATCG253.8904796E-545.09
TATTACG302.1652486E-644.9999961
AACGTAG800.042.1875041
CGTTTTT33950.041.818851
CGTTAGG1400.040.178572
GGACGTA403.4576806E-739.3750048
TGCGAAG1150.039.1304361
CGGGATA1850.038.918926
CTCGTAG356.247563E-638.5714261
ATAATCG356.247563E-638.57142629
TACGGGT2050.038.4146354
GCGTTAG950.037.8947371
TACGGGA3400.037.720594
GGGCGAT12600.037.678577
CATACGG900.037.52
GGCGATA2100.037.58
GTTAGCG601.546141E-1037.4999961
TAACGAC301.1398272E-437.49999629
Produced by FastQC (version 0.11.5)