##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547057_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1014147
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.638232918896374	31.0	31.0	33.0	30.0	34.0
2	32.04082938666682	33.0	31.0	34.0	30.0	34.0
3	32.144765995462194	33.0	31.0	34.0	30.0	34.0
4	35.8170146931362	37.0	35.0	37.0	35.0	37.0
5	35.763483991965664	37.0	35.0	37.0	35.0	37.0
6	35.824013678490395	37.0	35.0	37.0	35.0	37.0
7	36.127699436077805	37.0	35.0	37.0	35.0	37.0
8	36.07482643048789	37.0	35.0	37.0	35.0	37.0
9	37.87976595108993	39.0	38.0	39.0	35.0	39.0
10	37.33583987331225	39.0	37.0	39.0	34.0	39.0
11	37.10026357125742	39.0	37.0	39.0	33.0	39.0
12	36.4896016060788	39.0	35.0	39.0	33.0	39.0
13	36.214901784455314	39.0	35.0	39.0	32.0	39.0
14	37.25813910606648	40.0	35.0	41.0	32.0	41.0
15	37.47087059371077	40.0	35.0	41.0	33.0	41.0
16	37.594069696010536	40.0	35.0	41.0	33.0	41.0
17	37.57693411310195	39.0	35.0	41.0	33.0	41.0
18	37.48797166485726	39.0	35.0	41.0	33.0	41.0
19	37.43997960847885	39.0	35.0	41.0	33.0	41.0
20	37.274775747500115	39.0	35.0	41.0	33.0	41.0
21	37.195257689467105	39.0	35.0	41.0	32.0	41.0
22	37.11756678272479	39.0	35.0	41.0	32.0	41.0
23	37.07646721826323	39.0	35.0	41.0	32.0	41.0
24	37.00707688333151	39.0	35.0	41.0	32.0	41.0
25	36.88741572967233	38.0	35.0	41.0	32.0	41.0
26	36.773459863313704	38.0	35.0	41.0	32.0	41.0
27	36.69126960884369	38.0	35.0	41.0	31.0	41.0
28	36.718403742258275	38.0	35.0	41.0	31.0	41.0
29	36.741839200825915	38.0	35.0	41.0	31.0	41.0
30	36.70167441209213	38.0	35.0	41.0	31.0	41.0
31	36.56601952182474	38.0	35.0	41.0	31.0	41.0
32	36.45246990820857	38.0	35.0	41.0	31.0	41.0
33	36.35214322972902	38.0	35.0	41.0	31.0	41.0
34	36.216772321961216	38.0	35.0	41.0	30.0	41.0
35	36.12667690186925	38.0	35.0	41.0	30.0	41.0
36	36.01709416879407	38.0	35.0	41.0	30.0	41.0
37	35.93537623243967	38.0	35.0	41.0	30.0	41.0
38	35.87886174292287	38.0	35.0	41.0	30.0	41.0
39	35.81079863175654	38.0	35.0	41.0	29.0	41.0
40	35.68129570959634	38.0	35.0	41.0	29.0	41.0
41	35.6425863311729	38.0	35.0	40.0	29.0	41.0
42	35.56723039164934	38.0	35.0	40.0	29.0	41.0
43	35.46150015727503	38.0	35.0	40.0	28.0	41.0
44	35.33205738418592	37.0	34.0	40.0	28.0	41.0
45	35.2356403953273	37.0	34.0	40.0	28.0	41.0
46	35.21619351040825	37.0	34.0	40.0	28.0	41.0
47	35.16372675756079	37.0	34.0	40.0	28.0	41.0
48	35.10014228706489	37.0	34.0	40.0	28.0	41.0
49	35.03971613582646	36.0	34.0	40.0	27.0	41.0
50	34.93291998102839	36.0	34.0	40.0	27.0	41.0
51	34.57240321176319	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	0.0
14	9.0
15	24.0
16	59.0
17	152.0
18	338.0
19	635.0
20	1182.0
21	1937.0
22	3043.0
23	4319.0
24	6494.0
25	9339.0
26	12937.0
27	15409.0
28	16606.0
29	18033.0
30	21266.0
31	25462.0
32	32283.0
33	42727.0
34	78621.0
35	135948.0
36	66276.0
37	88279.0
38	138035.0
39	294514.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.262679867908695	22.88534107974485	29.940629908681878	14.911349143664577
2	30.914551835187602	24.987403206832933	30.63618982257996	13.461855135399503
3	28.980414081982197	24.790686162854104	32.476751397972876	13.752148357190821
4	25.7010078420584	27.45134581081441	32.24976260837926	14.597883738747933
5	24.042668370561664	32.05570790033397	29.72547372323736	14.176150005867
6	21.634240401046398	40.87829476397406	26.15666170683343	11.330803128146115
7	88.69394673553242	3.2251734709070776	6.594507502364055	1.4863722911964439
8	89.83845537185438	2.2889186676093307	6.140036898003938	1.7325890625323546
9	85.77780144298607	3.63004574287554	7.949044862332581	2.643107951805803
10	46.63968832920671	23.566603263629435	18.026380790950427	11.767327616213429
11	39.976058697604984	21.160048789771107	22.57266451510481	16.291227997519098
12	34.827199607157546	21.702376479938312	25.859959157794677	17.61046475510947
13	23.130374590665852	31.89517890404448	27.659698248873195	17.314748256416475
14	18.650057634642707	33.76423733442982	30.052842438029202	17.53286259289827
15	17.65414678542657	23.737978813722272	41.59742128113577	17.010453119715386
16	20.927735328310394	21.335467146281555	38.68807973597516	19.048717789432892
17	21.525774863012956	20.865416946458453	28.418759805038125	29.19004838549047
18	24.216016021346018	23.35302475873813	31.916576196547442	20.514383023368406
19	27.28421027720833	25.143297766497362	26.88771943317882	20.684772523115484
20	28.899755163699147	25.118153482680516	26.112683861412595	19.86940749220774
21	25.11430788633206	25.641647611243734	29.171214823886476	20.072829678537726
22	24.17499632696246	22.44546402050196	28.95300188237011	24.426537770165467
23	21.32580385289312	27.128315717543906	28.075318469610423	23.47056195995255
24	21.991683651383873	24.67088104584444	33.73189488308894	19.60554041968275
25	20.216793029018476	25.855620536273342	32.04338227101199	21.88420416369619
26	19.62624747694368	30.251235767595823	27.49769017706506	22.624826578395442
27	20.2338517000001	28.32488781212191	30.101553325109677	21.339707162768317
28	18.50924964526839	26.80153863295952	35.18030423597368	19.50890748579841
29	18.678455884600556	24.548413592901227	34.55199295565633	22.221137566841886
30	21.37057053859056	26.266902135489232	31.20297156132198	21.159555764598228
31	27.043613992843245	24.446850407288096	26.803806548754768	21.70572905111389
32	27.075266208942097	25.494134479518255	26.790297659017874	20.640301652521774
33	25.55191702977971	25.561876138271867	26.84038901658241	22.045817815366018
34	20.621073670779484	27.15148790066923	28.90517844060082	23.322259987950464
35	21.368598437899042	26.183580881272633	29.71847276578248	22.729347915045846
36	27.251276195659997	24.423974039266497	27.67153085302229	20.653218912051212
37	22.322109122247564	28.470527448190452	28.263161060477426	20.94420236908456
38	22.03073124507591	29.331645215141393	25.477568833709512	23.160054706073183
39	22.27034147909524	27.758697703587348	27.243486397928507	22.727474419388905
40	23.549939012786115	25.53288625810657	26.962264839318166	23.954909889789153
41	20.062870570045565	24.079743863562186	28.404560680059205	27.452824886333044
42	22.949730167322883	25.652001139874198	26.367775085860334	25.030493606942585
43	22.849448847159238	26.033109598509878	26.719795059296136	24.397646495034746
44	21.58641695927711	28.572090633803583	27.513072562458895	22.328419844460416
45	20.66160033999016	30.381394413235952	25.352044624694447	23.604960622079442
46	22.87962198773945	27.897730802339304	27.49009758940272	21.732549620518522
47	22.33196962570515	26.029165397126846	28.538860737151516	23.100004240016485
48	23.567589313975194	24.220650457971082	29.672522819670128	22.5392374083836
49	22.493583277374977	23.485155505069777	29.88314317352415	24.138118044031092
50	20.515664888817895	28.306251460587074	28.41728072951949	22.760802921075545
51	20.618904360018814	29.54305440927203	26.20931679529693	23.628724435412224
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	465.0
1	769.0
2	1073.0
3	5415.5
4	9758.0
5	6810.5
6	3863.0
7	3953.0
8	4043.0
9	4116.0
10	4189.0
11	4230.5
12	4272.0
13	4157.0
14	4042.0
15	3761.5
16	3481.0
17	3388.5
18	3296.0
19	3311.5
20	3327.0
21	3566.0
22	3805.0
23	4218.0
24	4631.0
25	5218.0
26	6830.0
27	7855.0
28	9776.5
29	11698.0
30	12677.5
31	13657.0
32	16297.0
33	18937.0
34	21672.5
35	24408.0
36	27095.5
37	29783.0
38	34609.5
39	39436.0
40	48704.0
41	57972.0
42	67822.0
43	77672.0
44	79638.0
45	81604.0
46	82757.0
47	83910.0
48	82162.0
49	80414.0
50	78261.5
51	76109.0
52	72171.5
53	68234.0
54	61921.5
55	55609.0
56	52686.0
57	49763.0
58	46092.0
59	42421.0
60	40984.5
61	39548.0
62	35720.5
63	31893.0
64	27512.0
65	23131.0
66	19408.5
67	15686.0
68	12859.5
69	10033.0
70	8625.0
71	7217.0
72	6114.0
73	5011.0
74	4210.0
75	2337.5
76	1266.0
77	1023.0
78	780.0
79	552.5
80	325.0
81	275.5
82	226.0
83	137.5
84	49.0
85	37.0
86	25.0
87	16.5
88	8.0
89	6.5
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1014147.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.294986590807476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10893203430358	17.96251537770587
2	5.739664369079005	2.674108090268618
3	2.1257219571958763	1.485559934659889
4	1.2629374238480797	1.1768044141427982
5	0.8883884202392115	1.0347498168450535
6	0.7301785503939552	1.0205699724193456
7	0.5998397782388847	0.9781281713495423
8	0.5163023404163832	0.9621804877441732
9	0.5027859881801047	1.0541153567431283
>10	9.601269555246063	57.10127976428957
>50	0.8826772697759042	12.433188399708026
>100	0.040450718998755866	1.4742515150426363
>500	4.2579704209216705E-4	0.07548310589702256
>1k	0.0	0.0
>5k	4.2579704209216705E-4	0.5670655931843337
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5717	0.5637249826701652	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.860503457585537E-5	0.0	0.0	0.028792670096149768	0.0
2	9.860503457585537E-5	0.0	0.0	0.10166179064770689	0.0
3	9.860503457585537E-5	0.0	0.0	0.16141644160067525	0.0
4	9.860503457585537E-5	0.0	0.0	0.27678433205442604	0.0
5	9.860503457585537E-5	0.0	0.0	0.5375946485075634	0.0
6	9.860503457585537E-5	0.0	0.0	0.8823178493847539	0.0
7	9.860503457585537E-5	0.0	0.0	1.1018126563506079	0.0
8	9.860503457585537E-5	0.0	0.0	1.6414780105842643	0.0
9	9.860503457585537E-5	0.0	0.0	1.9139237211173528	0.0
10	9.860503457585537E-5	0.0	0.0	2.301638717069616	0.0
11	9.860503457585537E-5	0.0	0.0	2.594101249621603	0.0
12	9.860503457585537E-5	0.0	0.0	2.8644762544285984	0.0
13	9.860503457585537E-5	0.0	0.0	2.9850702117148695	0.0
14	9.860503457585537E-5	0.0	0.0	3.0329922585187354	0.0
15	9.860503457585537E-5	0.0	0.0	3.093338539679159	0.0
16	9.860503457585537E-5	0.0	0.0	3.2196515889708297	0.0
17	9.860503457585537E-5	0.0	0.0	3.3625302840712443	0.0
18	9.860503457585537E-5	0.0	0.0	3.557768252531438	0.0
19	9.860503457585537E-5	0.0	0.0	3.6657407653919996	0.0
20	9.860503457585537E-5	0.0	0.0	3.788109613300636	0.0
21	9.860503457585537E-5	0.0	0.0	3.936510190337298	0.0
22	9.860503457585537E-5	0.0	0.0	4.089841019102753	0.0
23	9.860503457585537E-5	0.0	0.0	4.256877947674252	0.0
24	9.860503457585537E-5	0.0	0.0	4.38752961848726	0.0
25	9.860503457585537E-5	0.0	0.0	4.501221223353222	0.0
26	9.860503457585537E-5	0.0	0.0	4.612644912423939	0.0
27	9.860503457585537E-5	0.0	0.0	4.727716987773961	0.0
28	9.860503457585537E-5	0.0	0.0	4.846930474576171	0.0
29	9.860503457585537E-5	0.0	0.0	4.981329136703062	0.0
30	9.860503457585537E-5	0.0	0.0	5.167692652051429	0.0
31	9.860503457585537E-5	0.0	0.0	5.311261582393874	0.0
32	9.860503457585537E-5	0.0	0.0	5.449900261007526	0.0
33	9.860503457585537E-5	0.0	0.0	5.598103627975037	0.0
34	9.860503457585537E-5	0.0	0.0	5.7579423890224986	0.0
35	9.860503457585537E-5	0.0	0.0	5.931783064979732	0.0
36	9.860503457585537E-5	0.0	0.0	6.086987389402128	0.0
37	9.860503457585537E-5	0.0	0.0	6.250868956867199	0.0
38	9.860503457585537E-5	0.0	0.0	6.4449236649124835	0.0
39	9.860503457585537E-5	0.0	0.0	6.721412181863181	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	25	3.8904796E-5	45.0	14
TCTAGCG	45	3.8380676E-10	45.0	1
GCGATCG	25	3.8904796E-5	45.0	9
TATTACG	30	2.1652486E-6	44.999996	1
AACGTAG	80	0.0	42.187504	1
CGTTTTT	3395	0.0	41.81885	1
CGTTAGG	140	0.0	40.17857	2
GGACGTA	40	3.4576806E-7	39.375004	8
TGCGAAG	115	0.0	39.130436	1
CGGGATA	185	0.0	38.91892	6
CTCGTAG	35	6.247563E-6	38.571426	1
ATAATCG	35	6.247563E-6	38.571426	29
TACGGGT	205	0.0	38.414635	4
GCGTTAG	95	0.0	37.894737	1
TACGGGA	340	0.0	37.72059	4
GGGCGAT	1260	0.0	37.67857	7
CATACGG	90	0.0	37.5	2
GGCGATA	210	0.0	37.5	8
GTTAGCG	60	1.546141E-10	37.499996	1
TAACGAC	30	1.1398272E-4	37.499996	29
>>END_MODULE