FastQCFastQC Report
Thu 26 May 2016
SRR1547056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547056_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences951688
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85260.8958818436294248No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11950.1255663620850531No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG750.045.0000041
GTAAGCG302.1651067E-645.0000041
GCGTATT253.8902974E-545.00000413
CGCCCTT253.8902974E-545.00000445
TGCGTAA253.8902974E-545.00000413
ATTTCGC302.1651067E-645.00000435
AAGTATC207.032675E-445.023
CGTATCA207.032675E-445.043
TTTCGCG207.032675E-445.01
ACGATTG207.032675E-445.016
CCGTACT207.032675E-445.020
ACGGCCA207.032675E-445.030
TCCACCG207.032675E-445.031
ACGTCGG207.032675E-445.02
CCAACGG207.032675E-445.02
TAATGCG551.8189894E-1245.01
TTTAGCG650.044.9999961
CGTTTTT45500.043.6153831
CGACGGT800.042.187528
GACGGTC800.042.187529