##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547056_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	951688
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.613787291633393	31.0	31.0	33.0	30.0	34.0
2	31.982166424290313	31.0	31.0	34.0	30.0	34.0
3	32.028353830246886	33.0	31.0	34.0	30.0	34.0
4	35.733079538672335	37.0	35.0	37.0	33.0	37.0
5	35.671009826749945	37.0	35.0	37.0	33.0	37.0
6	35.724795311068334	37.0	35.0	37.0	33.0	37.0
7	36.09132614890594	37.0	35.0	37.0	35.0	37.0
8	36.04967069039433	37.0	35.0	37.0	35.0	37.0
9	37.86071905918747	39.0	38.0	39.0	35.0	39.0
10	37.39027391329932	39.0	37.0	39.0	35.0	39.0
11	37.02741024369331	39.0	37.0	39.0	33.0	39.0
12	36.199647363421626	38.0	35.0	39.0	32.0	39.0
13	35.79705743899261	38.0	35.0	39.0	31.0	39.0
14	36.79037457654189	39.0	35.0	41.0	31.0	41.0
15	37.07091504778877	39.0	35.0	41.0	32.0	41.0
16	37.25828737989762	39.0	35.0	41.0	33.0	41.0
17	37.23054719613991	39.0	35.0	41.0	33.0	41.0
18	37.15213494338481	38.0	35.0	41.0	32.0	41.0
19	37.0734127150915	38.0	35.0	41.0	32.0	41.0
20	36.85590235455317	38.0	35.0	41.0	32.0	41.0
21	36.77255150847757	38.0	35.0	41.0	32.0	41.0
22	36.66716402854717	38.0	35.0	41.0	32.0	41.0
23	36.651309042459296	38.0	35.0	41.0	32.0	41.0
24	36.53754066458755	38.0	35.0	41.0	32.0	41.0
25	36.43510057918141	37.0	35.0	40.0	32.0	41.0
26	36.27672199292205	37.0	35.0	40.0	31.0	41.0
27	36.1856911088508	37.0	35.0	40.0	31.0	41.0
28	36.229253704995756	37.0	35.0	40.0	31.0	41.0
29	36.275895041232	37.0	35.0	40.0	31.0	41.0
30	36.253692386580475	37.0	35.0	40.0	31.0	41.0
31	36.09728923764931	37.0	35.0	40.0	31.0	41.0
32	35.901675759282455	37.0	35.0	40.0	30.0	41.0
33	35.74341590941569	37.0	35.0	40.0	30.0	41.0
34	35.592550289590704	37.0	35.0	40.0	30.0	41.0
35	35.48810744697842	37.0	35.0	40.0	29.0	41.0
36	35.341577281630116	37.0	35.0	40.0	29.0	41.0
37	35.24721862627248	37.0	35.0	40.0	28.0	41.0
38	35.18636359815402	37.0	34.0	40.0	28.0	41.0
39	35.133018384176324	36.0	34.0	40.0	27.0	41.0
40	34.9966921932398	36.0	34.0	40.0	27.0	41.0
41	34.988058061045216	36.0	34.0	40.0	27.0	41.0
42	34.89839632316473	36.0	34.0	40.0	27.0	41.0
43	34.77054349744874	36.0	34.0	40.0	26.0	41.0
44	34.57458746984306	35.0	34.0	40.0	26.0	41.0
45	34.501499440993264	35.0	34.0	40.0	25.0	41.0
46	34.489752944242234	35.0	34.0	40.0	25.0	41.0
47	34.4509702759728	35.0	34.0	40.0	25.0	41.0
48	34.38868725884954	35.0	34.0	40.0	25.0	41.0
49	34.33954089995881	36.0	34.0	40.0	25.0	41.0
50	34.19931216953455	35.0	34.0	40.0	24.0	41.0
51	33.82549112734426	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	2.0
13	4.0
14	9.0
15	23.0
16	54.0
17	156.0
18	348.0
19	787.0
20	1311.0
21	2052.0
22	3387.0
23	4911.0
24	7360.0
25	10920.0
26	14418.0
27	16662.0
28	17368.0
29	18783.0
30	21996.0
31	26568.0
32	33088.0
33	44536.0
34	83090.0
35	158970.0
36	63645.0
37	77704.0
38	114340.0
39	228971.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.63475004413211	23.053143467186725	29.72066475567623	14.591441733004935
2	31.35397315086457	25.536835601583714	30.025071241835562	13.08412000571616
3	27.78683770311278	24.808025319222267	33.800783450038246	13.6043535276267
4	25.37207572229554	26.852077571641125	33.56236497675709	14.213481729306244
5	23.393065794672204	31.715436151343717	31.074259631307736	13.81723842267634
6	20.85032069333647	40.00092467279192	28.01642975428922	11.132324879582384
7	86.15565185228773	3.648884928674103	8.403069073057557	1.7923941459806154
8	87.35520464690109	2.6678911576062743	7.720177200931398	2.2567269945612427
9	83.8139180067417	3.889299854574188	9.160565227259355	3.1362169114247522
10	52.61377678398803	22.976227503131277	13.34124208774304	11.068753625137651
11	43.51184421785291	21.515980027067695	21.51871201486201	13.453463740217384
12	38.14926740696531	21.101978799774717	25.248400736375785	15.500353056884189
13	21.736010120964014	36.91545968846933	26.681748640310687	14.666781550255967
14	15.570859357268349	39.8006489521776	29.53089668042468	15.09759501012937
15	14.45967586015585	23.901740906683703	46.73191213927254	14.906671093887914
16	16.816330562116995	19.98165365119661	44.29939223779222	18.902623548894177
17	17.118635519203774	20.567244727263557	29.29100713679273	33.023112616739944
18	21.591845226586866	23.78909894839485	33.86120241087415	20.75785341414413
19	27.606736661594976	25.65945982296719	26.29800943166248	20.435794083775356
20	29.911798824824942	24.23462311177613	24.988021284286447	20.86555677911248
21	22.257504560318086	28.44871428451341	28.463635140928538	20.83014601423996
22	22.164406822403983	24.120825312497374	27.350034885382605	26.364732979716045
23	19.976925210783367	29.702591605652273	25.850173586301395	24.47030959726297
24	20.35709182000824	24.43353283849329	36.440724270979565	18.76865107051891
25	18.266595775085953	26.166979093988786	33.98508754970116	21.5813375812241
26	17.489765553416667	33.904598986222375	27.5266684039307	21.07896705643026
27	18.97922428358874	31.663843612612535	29.410058758752868	19.946873345045855
28	16.485865115458008	27.52803439782786	37.88930826069048	18.096792226023656
29	16.55122266961441	24.611532350938543	36.2820588260018	22.55518615344525
30	19.361177192525282	28.15912357831558	31.645875539042205	20.83382369011693
31	27.940039172501912	25.70422239221257	24.89366262892881	21.462075806356708
32	28.160699725119997	27.573952808063147	25.064411866073755	19.2009356007431
33	25.655151688368456	27.315779961499985	25.014080244786108	22.01498810534545
34	18.847038104925144	28.385143030068676	28.391027311471827	24.376791553534353
35	19.64772068156791	26.341406007010704	29.86398903842436	24.14688427299703
36	28.030509999075328	24.67352745857886	26.59768747740856	20.69827506493725
37	20.786434209530853	29.589214112188028	28.75911012852952	20.865241549751598
38	20.51365573591345	31.193416329721508	24.553214919175193	23.739713015189853
39	21.423933053689865	29.21240994947924	27.07420919461	22.289447802220895
40	22.848454535520045	25.53872697774901	26.68931414497188	24.923504341759063
41	18.363476265330654	24.537768680491926	27.51405922949538	29.58469582468204
42	22.67665453383882	26.690469985961784	24.75580232176932	25.877073158430075
43	22.69294138415111	26.735757937475306	25.736585939929896	24.83471473844369
44	20.962752498718068	30.267062314540063	27.1445053420869	21.62567984465497
45	18.30526391002093	33.81150124830827	23.521889526819713	24.361345314851086
46	22.959730499911736	29.96843503333025	26.062322946175637	21.009511520582375
47	21.687359722934406	26.44858398971091	27.812371281344305	24.051685006010374
48	23.591345062667596	24.14835534334782	29.32999050108859	22.930309092895993
49	21.508204369499246	22.899101386168578	30.66120409209741	24.931490152234765
50	19.17771370449139	30.29890047998924	27.725788283555115	22.797597531964257
51	18.71338085591076	31.52945082842276	24.67352745857886	25.08364085708762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	652.0
1	845.5
2	1039.0
3	6545.0
4	12051.0
5	8151.5
6	4252.0
7	4354.0
8	4456.0
9	4905.0
10	5354.0
11	5569.0
12	5784.0
13	5797.0
14	5810.0
15	5602.0
16	5394.0
17	5135.0
18	4876.0
19	4577.0
20	4278.0
21	4162.5
22	4047.0
23	4292.0
24	4537.0
25	4981.0
26	5553.5
27	5682.0
28	7007.0
29	8332.0
30	9414.5
31	10497.0
32	12195.0
33	13893.0
34	15772.5
35	17652.0
36	19874.5
37	22097.0
38	26389.5
39	30682.0
40	43496.0
41	56310.0
42	69406.0
43	82502.0
44	86135.5
45	89769.0
46	91448.5
47	93128.0
48	91274.0
49	89420.0
50	83479.0
51	77538.0
52	70150.5
53	62763.0
54	55856.0
55	48949.0
56	45282.5
57	41616.0
58	39316.5
59	37017.0
60	32153.5
61	27290.0
62	24278.0
63	21266.0
64	18667.5
65	16069.0
66	13689.5
67	11310.0
68	9560.0
69	7810.0
70	6307.0
71	4804.0
72	4121.5
73	3439.0
74	2628.5
75	1424.5
76	1031.0
77	798.0
78	565.0
79	451.5
80	338.0
81	205.0
82	72.0
83	57.5
84	43.0
85	29.0
86	15.0
87	11.5
88	8.0
89	7.5
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	951688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.98392514913043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62565896168734	21.216288962232277
2	7.42137495922615	4.005156528067787
3	2.6132490505651247	2.115471503294565
4	1.4425484179683559	1.5570247413781857
5	0.9809445800241965	1.3234867561409054
6	0.7406369878979567	1.1991175826469533
7	0.5704233919259993	1.0774583477730981
8	0.49685309949666384	1.0725637477545147
9	0.4096197996827123	0.9947835012816101
>10	5.173656363098347	35.02430133406515
>50	1.4198596032906856	24.831478932465128
>100	0.10203523931151162	4.136956064970479
>500	0.002354659368727191	0.4164912763919749
>1k	3.924432281211985E-4	0.12654642138022124
>5k	3.924432281211985E-4	0.9028743001571264
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8526	0.8958818436294248	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1195	0.1255663620850531	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07250275300308505	0.0
2	0.0	0.0	0.0	0.26826018611141467	0.0
3	0.0	0.0	0.0	0.409482939786989	0.0
4	0.0	0.0	0.0	0.64737603080001	0.0
5	0.0	0.0	0.0	1.2464168929312969	0.0
6	0.0	0.0	0.0	1.925841242087743	0.0
7	0.0	0.0	0.0	2.4164432040752852	0.0
8	0.0	0.0	0.0	3.4432503089247737	0.0
9	0.0	0.0	0.0	3.946356368894007	0.0
10	0.0	0.0	0.0	4.701120535301485	0.0
11	0.0	0.0	0.0	5.313401030589858	0.0
12	0.0	0.0	0.0	5.838678222274527	0.0
13	0.0	0.0	0.0	6.066694126646548	0.0
14	0.0	0.0	0.0	6.164520304973899	0.0
15	0.0	0.0	0.0	6.285988685367474	0.0
16	0.0	0.0	0.0	6.526298534813931	0.0
17	0.0	0.0	0.0	6.824715663116484	0.0
18	0.0	0.0	0.0	7.217596523230302	0.0
19	0.0	0.0	0.0	7.424702213330419	0.0
20	0.0	0.0	0.0	7.643681542690461	0.0
21	0.0	0.0	0.0	7.915514328225217	0.0
22	0.0	0.0	0.0	8.196068459411068	0.0
23	0.0	0.0	0.0	8.510457208665025	0.0
24	0.0	0.0	0.0	8.756441186607375	0.0
25	0.0	0.0	0.0	8.948310790931481	0.0
26	0.0	0.0	0.0	9.140915930430982	0.0
27	0.0	0.0	0.0	9.323223577474971	0.0
28	0.0	0.0	0.0	9.523919603903801	0.0
29	1.0507645362765948E-4	0.0	0.0	9.753406578626608	0.0
30	1.0507645362765948E-4	0.0	0.0	10.054030312455343	0.0
31	1.0507645362765948E-4	0.0	0.0	10.31136254738948	0.0
32	1.0507645362765948E-4	0.0	0.0	10.525298206975394	0.0
33	1.0507645362765948E-4	0.0	0.0	10.75310395844016	0.0
34	1.0507645362765948E-4	0.0	0.0	10.988580291019746	0.0
35	1.0507645362765948E-4	0.0	0.0	11.26713796958667	0.0
36	1.0507645362765948E-4	0.0	0.0	11.519636687653938	0.0
37	1.0507645362765948E-4	0.0	0.0	11.773816628979246	0.0
38	1.0507645362765948E-4	0.0	0.0	12.025369658963863	0.0
39	1.0507645362765948E-4	0.0	0.0	12.291002933734585	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	75	0.0	45.000004	1
GTAAGCG	30	2.1651067E-6	45.000004	1
GCGTATT	25	3.8902974E-5	45.000004	13
CGCCCTT	25	3.8902974E-5	45.000004	45
TGCGTAA	25	3.8902974E-5	45.000004	13
ATTTCGC	30	2.1651067E-6	45.000004	35
AAGTATC	20	7.032675E-4	45.0	23
CGTATCA	20	7.032675E-4	45.0	43
TTTCGCG	20	7.032675E-4	45.0	1
ACGATTG	20	7.032675E-4	45.0	16
CCGTACT	20	7.032675E-4	45.0	20
ACGGCCA	20	7.032675E-4	45.0	30
TCCACCG	20	7.032675E-4	45.0	31
ACGTCGG	20	7.032675E-4	45.0	2
CCAACGG	20	7.032675E-4	45.0	2
TAATGCG	55	1.8189894E-12	45.0	1
TTTAGCG	65	0.0	44.999996	1
CGTTTTT	4550	0.0	43.615383	1
CGACGGT	80	0.0	42.1875	28
GACGGTC	80	0.0	42.1875	29
>>END_MODULE