FastQCFastQC Report
Thu 26 May 2016
SRR1547055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547055_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1053438
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92910.8819693233014188No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA207.0330186E-445.014
AAATGCG502.1827873E-1145.01
CGTTTTT50750.042.738921
ACGGGTA700.041.7857135
CGTTCAT451.9281288E-840.017
GTATGCG451.9281288E-840.01
AACGGGT800.039.3754
CGTAAGG1500.039.0000042
ACCCGCT3550.038.66197234
TAAACGG700.038.5714262
TAACGGG3000.038.2500043
GTAGGGC4500.037.54
ACCGGAC301.1398573E-437.49999613
TACGACG301.1398573E-437.4999961
TGTTACG301.1398573E-437.4999961
TGCGGGA5850.036.9230774
ATAGGGA10750.036.837214
TAAGGGA8950.036.703914
TTTAGCG1050.036.4285741
TCGATCA2100.036.42857417
Produced by FastQC (version 0.11.5)