FastQCFastQC Report
Thu 26 May 2016
SRR1547054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547054_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences619293
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140282.265163662434421No Hit
GAATCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTC28090.4535817456357492No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCG18830.304056399797834No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGC13820.22315769756803325No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTT9260.14952534583791516No Hit
GAACTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTCT9020.14564995890475108No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCT8580.13854508286061687No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6740.10883378303969203No Hit
GCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTCTGC6260.10108300917336381Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTC453.8380676E-1045.0000049
GTTTGCG351.2107193E-745.01
TCGTTGA207.030764E-445.043
CGTTGAA207.030764E-445.044
ATTACGG351.2107193E-745.02
ATCGTCA253.888714E-545.036
TACGACG253.888714E-545.01
ATGCGAG207.030764E-445.01
CGGAGAC207.030764E-445.031
TGCCGTA207.030764E-445.016
CGGACGG351.2107193E-745.02
TCGTTAG302.1638734E-644.9999961
CGTTTTT69250.044.3501781
CGGGTAC700.041.7857136
CCGATCG650.041.53846440
AGTACGG650.041.5384642
CGATGAA5300.041.17924519
GGCGCGA2650.040.754728
ACACCCG501.0804797E-940.536
GCGTAAG501.0804797E-940.51