##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547054_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	619293
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.563444120957286	31.0	31.0	33.0	30.0	34.0
2	31.91185270946063	31.0	31.0	34.0	30.0	34.0
3	31.832626882590308	31.0	31.0	34.0	30.0	34.0
4	35.6364418780771	37.0	35.0	37.0	33.0	37.0
5	35.590003439405905	37.0	35.0	37.0	33.0	37.0
6	35.674582144477654	37.0	35.0	37.0	33.0	37.0
7	36.02958373500104	37.0	35.0	37.0	35.0	37.0
8	35.96563339162561	37.0	35.0	37.0	35.0	37.0
9	37.75837446895088	39.0	38.0	39.0	35.0	39.0
10	37.318183799913776	39.0	37.0	39.0	34.0	39.0
11	36.979702660937555	39.0	37.0	39.0	33.0	39.0
12	36.31993741250103	38.0	35.0	39.0	32.0	39.0
13	35.993799380906935	38.0	35.0	39.0	31.0	39.0
14	37.072632824850274	39.0	35.0	41.0	32.0	41.0
15	37.28781691380332	39.0	35.0	41.0	32.0	41.0
16	37.44290182514577	39.0	35.0	41.0	33.0	41.0
17	37.374892013957854	39.0	35.0	41.0	32.0	41.0
18	37.20992002170217	39.0	35.0	41.0	32.0	41.0
19	37.02947231762671	38.0	35.0	41.0	32.0	41.0
20	36.64902719714255	38.0	35.0	41.0	32.0	41.0
21	36.61482044847915	38.0	35.0	40.0	32.0	41.0
22	36.5256930079946	37.0	35.0	40.0	32.0	41.0
23	36.43754894694434	37.0	35.0	40.0	32.0	41.0
24	36.35920961483498	37.0	35.0	40.0	31.0	41.0
25	36.30334268270431	37.0	35.0	40.0	31.0	41.0
26	36.11197607594467	37.0	35.0	40.0	31.0	41.0
27	36.003618642548844	37.0	35.0	40.0	31.0	41.0
28	36.03504318634314	37.0	35.0	40.0	31.0	41.0
29	35.99651538124926	37.0	35.0	40.0	31.0	41.0
30	35.964716216718095	37.0	35.0	40.0	31.0	41.0
31	35.74009394583824	37.0	35.0	40.0	30.0	41.0
32	35.356554974785766	37.0	35.0	40.0	29.0	41.0
33	35.02489451681191	37.0	34.0	40.0	25.0	41.0
34	34.66288493491772	37.0	34.0	40.0	23.0	41.0
35	34.43691273758948	37.0	34.0	40.0	23.0	41.0
36	34.291606719275045	36.0	34.0	40.0	21.0	41.0
37	34.15257882779234	36.0	34.0	40.0	21.0	41.0
38	34.08118289727157	36.0	34.0	40.0	21.0	41.0
39	33.99252050321899	36.0	34.0	40.0	20.0	41.0
40	33.88199446788515	36.0	33.0	40.0	19.0	41.0
41	33.83124950548448	36.0	33.0	40.0	18.0	41.0
42	33.73279045621378	36.0	33.0	40.0	18.0	41.0
43	33.60000193769346	35.0	33.0	40.0	18.0	41.0
44	33.421651786795586	35.0	33.0	40.0	18.0	41.0
45	33.39135433470102	35.0	33.0	40.0	18.0	41.0
46	33.35845875861668	35.0	33.0	40.0	18.0	41.0
47	33.26366356474237	35.0	33.0	40.0	18.0	41.0
48	33.22021563298794	35.0	33.0	40.0	18.0	41.0
49	33.1543938006727	35.0	33.0	40.0	18.0	41.0
50	33.02341218130998	35.0	33.0	40.0	17.0	41.0
51	32.65233096450307	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	2.0
14	4.0
15	17.0
16	46.0
17	120.0
18	336.0
19	649.0
20	1173.0
21	2103.0
22	3273.0
23	4847.0
24	7186.0
25	11024.0
26	15032.0
27	16041.0
28	14738.0
29	13882.0
30	15371.0
31	17565.0
32	21837.0
33	28489.0
34	49962.0
35	91985.0
36	42001.0
37	48834.0
38	70568.0
39	142106.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.396447239029023	22.174964031565025	26.630367209059365	19.79822152034659
2	36.3117296659255	24.995276872175207	26.971562733633352	11.721430728265942
3	25.793122157040365	24.869003847936273	37.34322848796934	11.994645507054011
4	22.909349855399626	24.594820222414914	39.564955521861215	12.93087440032424
5	23.148816472978055	29.091237911618574	35.07903367226822	12.680911943135156
6	19.094677317521754	39.13769411247988	31.86520758348633	9.90242098651204
7	78.88786083485523	3.2147949355151764	15.880528279828773	2.0168159498008214
8	79.3971512676552	3.9254440143841447	14.069753735307843	2.607650982652799
9	75.47784328258192	3.8329191513548513	15.798660730865679	4.890576835197556
10	47.05058833217879	20.84037765645664	21.095991719589918	11.013042291774653
11	38.51843957545136	20.158955454041948	28.20393577837954	13.118669192127152
12	33.15764912569656	19.65031091906416	30.6916112405598	16.500428714679483
13	20.56005800162443	32.60879745128719	32.790456213779265	14.040688333309111
14	15.038438994143322	36.18626401396431	33.78287821758037	14.992418774311997
15	13.588721332228848	23.12895511494559	47.986817225449016	15.29550632737654
16	15.240766486945597	20.99103332348339	45.69807829250452	18.070121897066493
17	16.043133056566116	22.195794236330784	34.11115578571048	27.64991692139262
18	19.935797756473914	24.137201615390453	36.46238533295225	19.46461529518338
19	23.745949009596426	26.862406001682565	29.882462743806244	19.509182244914765
20	26.40381854792481	25.145932539201958	29.89150531331696	18.55874359955627
21	19.516610069869998	29.302446499476016	32.312491825355686	18.8684516052983
22	19.587658830311337	24.251041106552147	32.81645360112257	23.34484646201394
23	17.97646671284836	29.33554876286346	31.1666690887835	21.521315435504683
24	18.70826894539418	25.644565657935743	38.697030323288004	16.950135073382068
25	16.408226800561284	27.97884038734492	36.61417132116785	18.998761490925943
26	15.65188045077209	32.24031274372551	32.9214119972291	19.186394808273306
27	18.257755214413855	29.549340942009678	33.24759039743708	18.945313446139387
28	15.489114199579198	27.844978063695216	39.90162976167985	16.764277975045736
29	15.937367288181846	24.994469499897466	38.858181829925414	20.209981381995277
30	18.20123915497188	29.09204528389631	33.624310302231734	19.082405258900067
31	24.754518458952386	26.468569804599763	28.234454450478207	20.542457285969647
32	25.07795179341604	28.389954351171415	28.089288914940102	18.442804940472442
33	23.865117157791225	28.038101512531227	27.13610520383728	20.960676125840273
34	18.212380892404724	29.79720423127663	29.561774475086917	22.42864040123173
35	19.887355419809364	27.90714572908139	30.428084929104642	21.77741392200461
36	25.887261764625148	28.30324256854187	27.056175348340773	18.753320318492218
37	19.560692596234738	31.098203919630933	29.93800995651493	19.403093527619397
38	20.64127965276533	32.33429087685474	26.196323872544987	20.828105597834952
39	19.722005577327696	31.886037788252086	28.10688963059489	20.28506700382533
40	22.55023066625975	27.718866513911834	27.52089883140936	22.210003988419054
41	17.679353714639113	27.02678699743094	28.689489466213892	26.604369821716055
42	21.25116867137203	28.12626656526071	26.81412513947356	23.808439623893698
43	21.095507296223275	29.01437607077748	27.22023339517805	22.669883237821193
44	19.455572725672663	31.483643445025216	28.54061001819817	20.520173811103952
45	17.726988679025922	33.89558738755323	25.965253926655073	22.41217000676578
46	22.367280108123293	31.23481130902497	26.833017650772735	19.564890932079
47	20.173165206130218	28.11076501752805	29.0876854735965	22.62838430274523
48	22.031574715037955	25.52781962657417	30.311015948831976	22.129589709555898
49	20.164607059986146	24.751288969841415	31.56970932983257	23.514394640339873
50	18.384189713108334	29.512040342777972	30.389008110861905	21.71476183325179
51	17.486876163625293	31.755566428168898	27.29984030176346	23.457717106442345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	532.0
1	623.5
2	715.0
3	9542.0
4	18369.0
5	12026.5
6	5684.0
7	5824.0
8	5964.0
9	6356.0
10	6748.0
11	6988.5
12	7229.0
13	7240.0
14	7251.0
15	7072.5
16	6894.0
17	6408.5
18	5923.0
19	5774.5
20	5626.0
21	5062.0
22	4498.0
23	4460.0
24	4422.0
25	4263.5
26	4583.5
27	5062.0
28	5603.0
29	6144.0
30	6869.5
31	7595.0
32	8972.5
33	10350.0
34	11682.5
35	13015.0
36	14160.0
37	15305.0
38	18238.0
39	21171.0
40	27960.5
41	34750.0
42	41512.0
43	48274.0
44	51773.5
45	55273.0
46	60315.0
47	65357.0
48	60032.0
49	54707.0
50	51092.0
51	47477.0
52	42242.0
53	37007.0
54	33192.5
55	29378.0
56	26015.0
57	22652.0
58	19957.5
59	17263.0
60	16029.5
61	14796.0
62	12377.0
63	9958.0
64	8455.5
65	6953.0
66	5725.0
67	4497.0
68	3796.5
69	3096.0
70	2658.5
71	2221.0
72	1982.0
73	1743.0
74	1210.5
75	489.5
76	301.0
77	228.0
78	155.0
79	134.5
80	114.0
81	64.5
82	15.0
83	15.5
84	16.0
85	10.0
86	4.0
87	3.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	619293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.25678827101649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4725076545906	28.491200262407695
2	8.118245058873978	6.049194745813871
3	3.64716547344592	4.076450155006087
4	2.069591098944495	3.084252695238215
5	1.4192111415044881	2.6437624505450166
6	0.992486463113959	2.2186114810852073
7	0.806254825142514	2.1026925718974043
8	0.6354172679496513	1.8938885292598326
9	0.5670371761313038	1.9013385611627158
>10	5.103670121219535	38.69699955086262
>50	0.14404377524154383	3.367019860570412
>100	0.020018168155917594	1.4736285215446427
>500	0.0026110654116414255	0.7417593229251557
>1k	0.0013055327058207127	0.9847969677480646
>5k	0.0	0.0
>10k+	4.351775686069042E-4	2.2744043239331333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14028	2.265163662434421	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTC	2809	0.4535817456357492	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCG	1883	0.304056399797834	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGC	1382	0.22315769756803325	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTT	926	0.14952534583791516	No Hit
GAACTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTCT	902	0.14564995890475108	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCT	858	0.13854508286061687	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	674	0.10883378303969203	No Hit
GCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTCTGC	626	0.10108300917336381	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06006849746404368	0.0
2	0.0	0.0	0.0	0.2475403403558574	0.0
3	0.0	0.0	0.0	0.41014511709320145	0.0
4	0.0	0.0	0.0	0.7579610943446802	0.0
5	0.0	0.0	0.0	1.6958047321703944	0.0
6	0.0	0.0	0.0	2.7011446924153835	0.0
7	0.0	0.0	0.0	3.546301992756256	0.0
8	0.0	0.0	0.0	4.969537843960775	0.0
9	0.0	0.0	0.0	5.82955079421211	0.0
10	0.0	0.0	0.0	6.916435354509093	0.0
11	0.0	0.0	0.0	7.457859203963229	0.0
12	0.0	0.0	0.0	8.1465477568776	0.0
13	0.0	0.0	0.0	8.431550170920712	0.0
14	0.0	0.0	0.0	8.592217254191473	0.0
15	0.0	0.0	0.0	8.75772857112869	0.0
16	0.0	0.0	0.0	8.97168222473046	0.0
17	0.0	0.0	0.0	9.212763586864376	0.0
18	0.0	0.0	0.0	9.556542702727143	0.0
19	0.0	0.0	0.0	9.775340589995366	0.0
20	0.0	0.0	0.0	9.975407440419962	0.0
21	0.0	0.0	0.0	10.192590583132702	0.0
22	0.0	0.0	0.0	10.417847448622865	0.0
23	0.0	0.0	0.0	10.688963059488804	0.0
24	0.0	0.0	0.0	10.89678068377973	0.0
25	0.0	0.0	0.0	11.080054190827282	0.0
26	0.0	0.0	0.0	11.250409741430955	0.0
27	0.0	0.0	0.0	11.434813569667346	0.0
28	0.0	0.0	0.0	11.619378872359286	0.0
29	0.0	0.0	0.0	11.820414569517176	0.0
30	0.0	0.0	0.0	12.109938268315643	0.0
31	0.0	0.0	0.0	12.334064812616967	0.0
32	0.0	0.0	0.0	12.557868408007195	0.0
33	0.0	0.0	0.0	12.790230149541493	0.0
34	0.0	0.0	0.0	13.021138620975854	0.0
35	0.0	0.0	0.0	13.266256844498484	0.0
36	0.0	0.0	0.0	13.5020095495993	0.0
37	0.0	0.0	0.0	13.747289247577479	0.0
38	0.0	0.0	0.0	14.012914726954769	0.0
39	0.0	0.0	0.0	14.282254118809675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATTC	45	3.8380676E-10	45.000004	9
GTTTGCG	35	1.2107193E-7	45.0	1
TCGTTGA	20	7.030764E-4	45.0	43
CGTTGAA	20	7.030764E-4	45.0	44
ATTACGG	35	1.2107193E-7	45.0	2
ATCGTCA	25	3.888714E-5	45.0	36
TACGACG	25	3.888714E-5	45.0	1
ATGCGAG	20	7.030764E-4	45.0	1
CGGAGAC	20	7.030764E-4	45.0	31
TGCCGTA	20	7.030764E-4	45.0	16
CGGACGG	35	1.2107193E-7	45.0	2
TCGTTAG	30	2.1638734E-6	44.999996	1
CGTTTTT	6925	0.0	44.350178	1
CGGGTAC	70	0.0	41.785713	6
CCGATCG	65	0.0	41.538464	40
AGTACGG	65	0.0	41.538464	2
CGATGAA	530	0.0	41.179245	19
GGCGCGA	265	0.0	40.75472	8
ACACCCG	50	1.0804797E-9	40.5	36
GCGTAAG	50	1.0804797E-9	40.5	1
>>END_MODULE