FastQCFastQC Report
Thu 26 May 2016
SRR1547053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547053_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences897893
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123441.3747740543695073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGA351.2115379E-745.029
ATTCGCG351.2115379E-745.01
GTATGCG207.032461E-445.01
CGTAGCG253.890122E-545.01
CGTTTTT74350.043.3355751
CGTTAGG800.042.18752
ATAGCGG1350.040.02
GGGCGAT11150.039.551577
GTATACG403.4570985E-739.3751
CTATGCG356.2467625E-638.5714261
TTGTACG356.2467625E-638.5714261
TACGTAG356.2467625E-638.5714261
ACGGGAT2400.038.4375045
CACGGGC1350.038.3333324
CGAGGGA4050.038.3333324
ATAGGGA9050.038.2872964
TAGGGAC6450.038.023265
ACGGGCT2200.037.8409085
CTCGATC3100.037.74193616
TATAGCG301.1397229E-437.5000041
Produced by FastQC (version 0.11.5)