FastQCFastQC Report
Thu 26 May 2016
SRR1547052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547052_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1040833
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99360.9546200014795841No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG207.032979E-445.01
GATTCGC207.032979E-445.010
TTACCCG207.032979E-445.014
CGTTTTT57800.043.1704141
TTAACGG800.042.18752
TATAGCG800.042.18751
GCGATAT1050.040.7142879
GCGATAC501.0804797E-940.59
CGGGTAC950.040.263166
GTGTACG451.9279469E-840.01
AACGTAG403.457808E-739.3751
GTTTACG800.039.3751
TATTCCG403.457808E-739.3751
CACGACC2500.038.727
ATTAGCG700.038.5714261
ACGGGTA700.038.5714265
TAACGGG3250.038.076923
TACGGGT950.037.8947374
GTATGCG950.037.8947371
ATAGGGC5850.037.692314