FastQCFastQC Report
Thu 26 May 2016
SRR1547051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547051_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences919336
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60810.661455659301931No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT351.2115743E-745.00000445
GTCGATC207.0325495E-445.022
CGTATGA207.0325495E-445.042
TTTCGTA253.8901937E-545.032
CGCATAT207.0325495E-445.011
ATGTACG253.8901937E-545.01
TTATACG551.8189894E-1245.01
CGTAGAA207.0325495E-445.022
ATACCGG253.8901937E-545.02
ACGTTAG302.1650267E-644.9999961
TACGTAG302.1650267E-644.9999961
ACGGGAT2100.042.8571435
CGTTTTT35350.042.6449781
CGTTAGG1600.042.18752
AAGTACG650.041.5384641
ATTAGCG556.002665E-1140.9090921
TACGGGA2100.040.7142874
GGGCGAT12000.040.68757
CTAAGCG501.0804797E-940.51
GTAACGA451.927583E-840.010
Produced by FastQC (version 0.11.5)