##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547051_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	919336
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.692386679081423	31.0	31.0	33.0	30.0	34.0
2	32.09168029969456	33.0	31.0	34.0	30.0	34.0
3	32.16654411444782	33.0	31.0	34.0	30.0	34.0
4	35.83783839640784	37.0	35.0	37.0	35.0	37.0
5	35.77746765056519	37.0	35.0	37.0	35.0	37.0
6	35.8297314583569	37.0	35.0	37.0	35.0	37.0
7	36.13777117397774	37.0	35.0	37.0	35.0	37.0
8	36.09572887388289	37.0	35.0	37.0	35.0	37.0
9	37.903586936658634	39.0	38.0	39.0	35.0	39.0
10	37.4544725758591	39.0	37.0	39.0	35.0	39.0
11	37.107356831452265	39.0	37.0	39.0	33.0	39.0
12	36.323302905575325	38.0	35.0	39.0	33.0	39.0
13	35.97343843817712	38.0	35.0	39.0	32.0	39.0
14	36.959323903338934	39.0	35.0	41.0	31.0	41.0
15	37.216221272744676	39.0	35.0	41.0	32.0	41.0
16	37.378678742048606	39.0	35.0	41.0	33.0	41.0
17	37.3564387775525	39.0	35.0	41.0	33.0	41.0
18	37.267088420338155	39.0	35.0	41.0	33.0	41.0
19	37.184106790118086	38.0	35.0	41.0	32.0	41.0
20	36.99838905470905	38.0	35.0	41.0	32.0	41.0
21	36.91813330490702	38.0	35.0	41.0	32.0	41.0
22	36.84405701506304	38.0	35.0	41.0	32.0	41.0
23	36.82760274807035	38.0	35.0	41.0	32.0	41.0
24	36.7427099558812	38.0	35.0	41.0	32.0	41.0
25	36.64389407137325	38.0	35.0	41.0	32.0	41.0
26	36.50710512804894	38.0	35.0	41.0	31.0	41.0
27	36.418797915016924	38.0	35.0	40.0	31.0	41.0
28	36.44974851414499	38.0	35.0	41.0	31.0	41.0
29	36.46910705117607	38.0	35.0	41.0	31.0	41.0
30	36.43281237762907	38.0	35.0	40.0	31.0	41.0
31	36.27103474681727	38.0	35.0	40.0	31.0	41.0
32	36.15151478893462	38.0	35.0	41.0	31.0	41.0
33	36.009681987869506	38.0	35.0	41.0	30.0	41.0
34	35.867300965044336	38.0	35.0	41.0	30.0	41.0
35	35.773598553738786	38.0	35.0	41.0	30.0	41.0
36	35.64335346380431	38.0	35.0	41.0	29.0	41.0
37	35.54072069406615	37.0	35.0	40.0	29.0	41.0
38	35.48627705213328	37.0	35.0	40.0	29.0	41.0
39	35.41982039210909	37.0	35.0	40.0	29.0	41.0
40	35.28274646048887	37.0	35.0	40.0	28.0	41.0
41	35.25996480068223	37.0	34.0	40.0	28.0	41.0
42	35.16610684232968	36.0	34.0	40.0	28.0	41.0
43	35.04172141632657	36.0	34.0	40.0	27.0	41.0
44	34.8979317681457	36.0	34.0	40.0	27.0	41.0
45	34.81861147610884	36.0	34.0	40.0	27.0	41.0
46	34.80373008345153	36.0	34.0	40.0	27.0	41.0
47	34.750366568914956	35.0	34.0	40.0	27.0	41.0
48	34.677727185707944	35.0	34.0	40.0	27.0	41.0
49	34.611736079083165	36.0	34.0	40.0	27.0	41.0
50	34.51048909212736	35.0	34.0	40.0	26.0	41.0
51	34.150691368552955	35.0	34.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	4.0
13	6.0
14	17.0
15	31.0
16	63.0
17	172.0
18	356.0
19	671.0
20	1146.0
21	1908.0
22	2857.0
23	4188.0
24	6276.0
25	9179.0
26	12702.0
27	15101.0
28	16399.0
29	17504.0
30	20247.0
31	24564.0
32	30391.0
33	40854.0
34	80008.0
35	140329.0
36	59478.0
37	77226.0
38	118793.0
39	238658.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.800055692369277	23.395254836099095	30.1716673773245	15.633022094207124
2	31.494469921769625	25.13716421417197	30.402486142172176	12.965879721886232
3	28.900641332439935	24.64235056606072	32.76941183636885	13.687596265130486
4	26.347820600955473	26.718305385626145	32.151030743928224	14.782843269490154
5	24.150582594394216	31.433121296239896	30.284357405779822	14.131938703586066
6	22.339275303044808	39.47011756311077	26.83034276912902	11.360264364715404
7	87.68709155303392	3.549953444660059	7.169631125072878	1.5933238772331333
8	89.4070285510412	2.4150038723600513	6.3872186012513374	1.7907489753474246
9	85.745146497037	3.737588868487691	7.907228695493269	2.610035938982048
10	53.05394328080266	22.015672180791356	13.297858454362713	11.632526084043265
11	44.03797958526589	20.222312625634153	21.324521176153226	14.415186612946734
12	38.7526432120574	20.411362113525414	24.026362505112388	16.809632169304802
13	24.146557950520812	32.10425785566974	25.66036791771452	18.08881627609492
14	17.81013688140136	34.52230740447453	29.74712183575972	17.920433878364385
15	16.7927721747	22.36984084165093	43.64215042160864	17.195236562040428
16	21.079888093145488	19.177428056771408	40.30158723252434	19.44109661755876
17	21.890364349922116	19.383446313426212	28.195458461324257	30.53073087532741
18	24.913524543801177	22.60772992681675	31.218183558568356	21.26056197081372
19	29.052163735565667	23.9092127361487	25.933608604471054	21.10501492381458
20	30.21528581497951	24.315157896568827	24.99347355047556	20.476082737976103
21	25.467620108426082	25.651122114221568	28.499373460845657	20.381884316506696
22	24.58350374618203	22.77078239076899	28.219715098723423	24.425998764325556
23	22.02818120904653	26.671423723208925	27.54292228303906	23.757472784705485
24	22.763494522133364	22.63144269342221	34.61922517991246	19.985837604531966
25	20.623145400593472	24.203881932177136	32.42666446217705	22.746308205052344
26	19.708463499743292	30.65843173768894	26.664244628732042	22.968860133835726
27	20.240369135985105	28.040672833436307	30.285989018160937	21.432969012417658
28	18.947153162717438	25.615226641837154	35.39978854303541	20.037831652410002
29	19.796135471688263	23.53481208176336	34.64217652740674	22.02687591914164
30	22.93633666037227	25.06667855930802	30.96289060800404	21.03409417231567
31	28.207532392944472	24.27882732754945	26.47367230261841	21.039967976887667
32	29.952813769938302	24.624402829868515	25.867582690115476	19.55520071007771
33	28.222216904374463	24.455367787185534	26.447566504520655	20.87484880391935
34	21.68043022355265	25.472623719728148	29.634758129780625	23.212187926938572
35	22.559325426177153	24.962472915234475	30.204517173264183	22.273684485324193
36	29.200640462246664	23.95587685024844	26.659676114064933	20.18380657343996
37	23.8661381692874	27.437085026584406	28.159998085574806	20.536778718553393
38	23.6711061026654	28.900641332439935	24.41762315410253	23.010629410792138
39	23.288547386374514	27.777221820966435	26.023129737114616	22.911101055544435
40	24.34648485428614	24.93832505199405	25.600650904565903	25.114539189153913
41	20.71897543444399	23.75214285092719	26.812068710460597	28.716813004168223
42	23.933143051071642	25.213088576972947	25.238650504277	25.61511786767841
43	23.674695649903843	25.125634153345462	26.416130772644603	24.783539424106095
44	22.821797471218357	27.35017447375062	27.647780572065056	22.180247482965967
45	20.68569054186935	30.417061879443423	24.677702167651436	24.219545411035792
46	23.51196950842782	27.866090308657554	26.61235935501275	22.009580827901875
47	23.36251381431816	25.190898648589855	28.250389411488293	23.196198125603694
48	24.562945430180044	22.590217287259502	29.21793555348643	23.628901729074027
49	22.979084904757347	22.09344570429092	30.442188710112518	24.485280680839214
50	21.012991985519985	28.427908838553044	27.002858584891705	23.55624059103527
51	20.956429422974843	29.845127352785056	25.218092188275016	23.98035103596509
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	488.0
1	927.0
2	1366.0
3	5677.0
4	9988.0
5	6930.5
6	3873.0
7	3880.5
8	3888.0
9	4026.0
10	4164.0
11	4131.5
12	4099.0
13	3882.5
14	3666.0
15	3525.0
16	3384.0
17	3181.5
18	2979.0
19	2789.5
20	2600.0
21	2764.5
22	2929.0
23	3062.0
24	3195.0
25	3429.0
26	4469.5
27	5276.0
28	5795.0
29	6314.0
30	7960.5
31	9607.0
32	11616.0
33	13625.0
34	15345.0
35	17065.0
36	18464.5
37	19864.0
38	24886.0
39	29908.0
40	39421.0
41	48934.0
42	59833.5
43	70733.0
44	72793.0
45	74853.0
46	74046.5
47	73240.0
48	71351.5
49	69463.0
50	70280.5
51	71098.0
52	66369.5
53	61641.0
54	59411.0
55	57181.0
56	52680.5
57	48180.0
58	45700.5
59	43221.0
60	41040.0
61	38859.0
62	34774.0
63	30689.0
64	27980.5
65	25272.0
66	21622.0
67	17972.0
68	15354.5
69	12737.0
70	10708.5
71	8680.0
72	7541.5
73	6403.0
74	5167.0
75	3071.5
76	2212.0
77	1668.0
78	1124.0
79	866.0
80	608.0
81	400.0
82	192.0
83	136.0
84	80.0
85	69.0
86	58.0
87	38.5
88	19.0
89	13.5
90	8.0
91	4.5
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	919336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.978527174747242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0264092800235	17.929319659889707
2	5.745824863915326	2.6406118555363336
3	2.0343533179697517	1.4023932900001548
4	1.2496697430657386	1.1486228060198191
5	0.8003200745997625	0.9195088291343192
6	0.6244967769524754	0.8610009695846713
7	0.5453986695393683	0.8772720704356956
8	0.46002450918911614	0.8456548548361494
9	0.39861141127493765	0.8243552831530955
>10	8.947515101418116	54.726803720594084
>50	1.1273845696819595	15.809549474487087
>100	0.039039499456399354	1.2580862549018454
>500	4.76091456785358E-4	0.09156679959589954
>1k	0.0	0.0
>5k	4.76091456785358E-4	0.6652541318311411
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6081	0.661455659301931	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.263224762219689E-4	0.0	0.0	0.024256637399166354	0.0
2	3.263224762219689E-4	0.0	0.0	0.10115996762881035	0.0
3	3.263224762219689E-4	0.0	0.0	0.17403865398505008	0.0
4	3.263224762219689E-4	0.0	0.0	0.2815075228208185	0.0
5	3.263224762219689E-4	0.0	0.0	0.531361765448106	0.0
6	3.263224762219689E-4	0.0	0.0	0.874761784592358	0.0
7	3.263224762219689E-4	0.0	0.0	1.1052542269638086	0.0
8	3.263224762219689E-4	0.0	0.0	1.5987625851701663	0.0
9	3.263224762219689E-4	0.0	0.0	1.871676949450473	0.0
10	3.263224762219689E-4	0.0	0.0	2.2621761793294293	0.0
11	3.263224762219689E-4	0.0	0.0	2.6183027750463377	0.0
12	3.263224762219689E-4	0.0	0.0	2.9074244889790024	0.0
13	3.263224762219689E-4	0.0	0.0	3.0322972232132757	0.0
14	3.263224762219689E-4	0.0	0.0	3.08440004525005	0.0
15	3.263224762219689E-4	0.0	0.0	3.149882088811925	0.0
16	3.263224762219689E-4	0.0	0.0	3.26507392291828	0.0
17	3.263224762219689E-4	0.0	0.0	3.402455685407729	0.0
18	3.263224762219689E-4	0.0	0.0	3.5908525283465456	0.0
19	3.263224762219689E-4	0.0	0.0	3.700279332039646	0.0
20	3.263224762219689E-4	0.0	0.0	3.8250432921151787	0.0
21	3.263224762219689E-4	0.0	0.0	3.973411244637434	0.0
22	3.263224762219689E-4	0.0	0.0	4.112750941984214	0.0
23	3.263224762219689E-4	0.0	0.0	4.26449089342743	0.0
24	3.263224762219689E-4	0.0	0.0	4.385121435470818	0.0
25	3.263224762219689E-4	0.0	0.0	4.494439465005177	0.0
26	3.263224762219689E-4	0.0	0.0	4.5935327236179155	0.0
27	3.263224762219689E-4	0.0	0.0	4.708180686930567	0.0
28	3.263224762219689E-4	0.0	0.0	4.820435618750925	0.0
29	3.263224762219689E-4	0.0	0.0	4.952161124985859	0.0
30	3.263224762219689E-4	0.0	0.0	5.125112037383503	0.0
31	3.263224762219689E-4	0.0	0.0	5.270216765143538	0.0
32	3.263224762219689E-4	0.0	0.0	5.39139117798063	0.0
33	3.263224762219689E-4	0.0	0.0	5.51909204034216	0.0
34	3.263224762219689E-4	0.0	0.0	5.66158618829242	0.0
35	3.263224762219689E-4	0.0	0.0	5.827575554530661	0.0
36	3.263224762219689E-4	0.0	0.0	5.96669770355996	0.0
37	3.263224762219689E-4	0.0	0.0	6.119525396590583	0.0
38	4.3509663496262517E-4	0.0	0.0	6.316515398069911	0.0
39	5.438707937032815E-4	0.0	0.0	6.627065621274485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTT	35	1.2115743E-7	45.000004	45
GTCGATC	20	7.0325495E-4	45.0	22
CGTATGA	20	7.0325495E-4	45.0	42
TTTCGTA	25	3.8901937E-5	45.0	32
CGCATAT	20	7.0325495E-4	45.0	11
ATGTACG	25	3.8901937E-5	45.0	1
TTATACG	55	1.8189894E-12	45.0	1
CGTAGAA	20	7.0325495E-4	45.0	22
ATACCGG	25	3.8901937E-5	45.0	2
ACGTTAG	30	2.1650267E-6	44.999996	1
TACGTAG	30	2.1650267E-6	44.999996	1
ACGGGAT	210	0.0	42.857143	5
CGTTTTT	3535	0.0	42.644978	1
CGTTAGG	160	0.0	42.1875	2
AAGTACG	65	0.0	41.538464	1
ATTAGCG	55	6.002665E-11	40.909092	1
TACGGGA	210	0.0	40.714287	4
GGGCGAT	1200	0.0	40.6875	7
CTAAGCG	50	1.0804797E-9	40.5	1
GTAACGA	45	1.927583E-8	40.0	10
>>END_MODULE