FastQCFastQC Report
Thu 26 May 2016
SRR1547050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547050_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2280910
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT496982.1788672065096826No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT34690.1520884208495732No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT31600.13854119627692457No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTC23200.10171378967166614No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG207.034748E-445.0000041
GCGTATA207.034748E-445.00000418
ACGTTAT351.2126293E-745.041
CCTCGCG253.892018E-545.034
CGTTTTT207500.044.132531
TATTGCG2100.041.7857131
TATAGCG2000.041.6251
ATAACGG1550.040.645162
GATTACG1000.040.51
CACGACC4200.040.1785727
TAGGGCG4600.040.1086925
TACGGGT3200.040.078134
TGCGTTG800.039.3750041
AAACACG4300.038.7209340
TGCGACA356.2509953E-638.57142630
CGGGATC6350.038.2677156
ATTGCGG5900.038.1355932
AGGGCGC3250.038.0769236
ATAGGGC10950.038.01374
CTAGCGG1600.037.9687542