##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547050_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2280910
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39724057503365	31.0	31.0	33.0	30.0	34.0
2	31.79951203686248	31.0	31.0	34.0	30.0	34.0
3	31.76540503570943	31.0	31.0	34.0	30.0	34.0
4	35.61589058752866	37.0	35.0	37.0	33.0	37.0
5	35.54965781201363	37.0	35.0	37.0	33.0	37.0
6	35.62482693311003	37.0	35.0	37.0	33.0	37.0
7	36.037643747451675	37.0	35.0	37.0	35.0	37.0
8	36.04555199459865	37.0	35.0	37.0	35.0	37.0
9	37.848189538385995	39.0	38.0	39.0	35.0	39.0
10	37.176416868705914	39.0	37.0	39.0	33.0	39.0
11	36.93331608875405	39.0	37.0	39.0	33.0	39.0
12	36.56254696590396	39.0	35.0	39.0	32.0	39.0
13	36.42107448342986	39.0	35.0	39.0	32.0	39.0
14	37.43568838752954	40.0	36.0	41.0	32.0	41.0
15	37.64382592912478	40.0	36.0	41.0	32.0	41.0
16	37.718645189858435	40.0	36.0	41.0	33.0	41.0
17	37.66095724951883	40.0	36.0	41.0	33.0	41.0
18	37.459737560885785	39.0	36.0	41.0	32.0	41.0
19	37.31108241885914	39.0	36.0	41.0	32.0	41.0
20	37.06584608774568	39.0	35.0	41.0	32.0	41.0
21	36.972747280690605	38.0	35.0	41.0	32.0	41.0
22	36.90448680570474	38.0	35.0	41.0	32.0	41.0
23	36.840977943013975	38.0	35.0	41.0	32.0	41.0
24	36.73578396341811	38.0	35.0	41.0	31.0	41.0
25	36.67771021215217	38.0	35.0	40.0	31.0	41.0
26	36.57010228373763	38.0	35.0	40.0	31.0	41.0
27	36.4731550126923	38.0	35.0	40.0	31.0	41.0
28	36.369545926844985	38.0	35.0	40.0	31.0	41.0
29	36.337138247453865	38.0	35.0	40.0	31.0	41.0
30	36.2563310257748	38.0	35.0	40.0	30.0	41.0
31	36.07485345761122	38.0	35.0	40.0	30.0	41.0
32	35.82127528048016	38.0	35.0	40.0	29.0	41.0
33	35.5543471684547	38.0	35.0	40.0	27.0	41.0
34	35.29430183567085	38.0	34.0	40.0	25.0	41.0
35	35.11452315084769	38.0	34.0	41.0	24.0	41.0
36	34.98013950572359	38.0	34.0	40.0	24.0	41.0
37	34.92868372710892	38.0	34.0	40.0	23.0	41.0
38	34.849265424764674	38.0	34.0	40.0	23.0	41.0
39	34.7782051900338	38.0	34.0	40.0	23.0	41.0
40	34.665918865715874	38.0	34.0	40.0	23.0	41.0
41	34.593002792745004	38.0	34.0	40.0	22.0	41.0
42	34.53856881683188	38.0	34.0	40.0	22.0	41.0
43	34.45274780679641	38.0	34.0	40.0	22.0	41.0
44	34.32770823925539	37.0	34.0	40.0	22.0	41.0
45	34.24119057744497	37.0	33.0	40.0	22.0	41.0
46	34.211948739757375	37.0	33.0	40.0	22.0	41.0
47	34.17515377634365	37.0	33.0	40.0	22.0	41.0
48	34.048421463363304	37.0	33.0	40.0	21.0	41.0
49	33.97093133880776	37.0	33.0	40.0	20.0	41.0
50	33.89969354336646	36.0	33.0	40.0	20.0	41.0
51	33.58351885870113	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	4.0
13	10.0
14	18.0
15	55.0
16	160.0
17	422.0
18	1062.0
19	2088.0
20	3611.0
21	5898.0
22	9031.0
23	13778.0
24	21801.0
25	34177.0
26	46306.0
27	51081.0
28	50546.0
29	51069.0
30	55888.0
31	65397.0
32	79949.0
33	101632.0
34	161273.0
35	237623.0
36	172174.0
37	208857.0
38	308665.0
39	597945.0
40	386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.13652884155885	23.443713254797427	28.318127414058424	19.101630489585297
2	35.146630073084864	24.02067595827981	28.695082225953676	12.13761174268165
3	26.14083852497468	23.717288275293633	37.60455256893082	12.537320630800863
4	24.437483285180036	25.479260470601645	36.82955487064373	13.253701373574584
5	22.059221977193314	29.68429267266135	34.968630941159454	13.287854408985888
6	19.75553616758224	38.53650516679746	31.573801684415432	10.13415698120487
7	82.68261351828875	3.3046459527118563	12.424821672051944	1.5879188569474463
8	83.9606998960941	2.3713342481728783	12.004638499546234	1.6633273561867852
9	80.44337567023689	4.09621598397131	13.188639621905294	2.271768723886519
10	37.99067039032667	28.30190581829182	21.286065649236487	12.42135814214502
11	29.655269168884345	25.5835171050151	29.821518604416656	14.939695121683888
12	28.29611865439671	22.487340578979442	31.85930176990762	17.357238996716223
13	22.681648990972903	26.846653309424745	32.901166639630674	17.570531059971678
14	16.619814021596643	30.22188512479668	34.43362517591663	18.724675677690044
15	16.51086627705609	28.362714881341216	39.32693530213818	15.799483539464513
16	20.581873024363084	26.82034801899242	37.51134415649894	15.086434800145557
17	21.150198824153517	26.50617516692899	31.751055499778598	20.592570509138895
18	21.38571885782429	27.68636202217536	34.09143718954277	16.83648193045758
19	23.038524097838142	28.820953040672364	30.960186942930672	17.18033591855882
20	24.469312686603153	29.53461557010141	30.426584126510907	15.56948761678453
21	22.79804990113595	27.815608682499526	33.86144126686278	15.524900149501732
22	21.964303720883333	22.84561863466774	34.86218219920996	20.327895445238962
23	19.008816656509904	27.27687633444546	34.31069178529622	19.403615223748417
24	18.37262320740406	27.153679890920728	37.26688032408118	17.20681657759403
25	19.630892933083725	28.143986391396414	34.15965557606394	18.065465099455917
26	19.250036169774347	32.329947257892684	30.256739634619517	18.16327693771346
27	18.30405408367713	31.508389195540374	33.096044999583505	17.091511721198994
28	17.044995199284497	29.136791894463176	36.14136463078333	17.676848275469002
29	18.086903911158267	28.418306728454873	35.402142127484204	18.092647232902657
30	20.282913398599682	28.09782060668768	34.33888228820953	17.280383706503105
31	23.56129790302993	26.896194939738965	31.83935359132978	17.703153565901328
32	23.563621537018122	28.146266183233887	31.362920939449605	16.92719134029839
33	23.023924661648202	28.31212103940971	30.787229658338124	17.876724640603967
34	19.12583135678304	29.332678623882575	31.416671416232994	20.124818603101392
35	19.339211104339935	29.73067766812369	31.84847275867965	19.081638468856728
36	23.152995953369487	29.73343972361908	29.60603443362518	17.50752988938625
37	19.334432309911396	31.513167989968917	30.036520511550215	19.115879188569473
38	19.96089280155727	32.33911026739328	28.35530555786945	19.344691373180005
39	19.545707634233704	30.478668601566916	29.387481312283253	20.588142451916124
40	21.640924017168587	28.90491075930221	29.55118790307377	19.90297732045543
41	19.41277823324901	27.97374732014854	30.012714223708958	22.600760222893495
42	20.39352714486762	28.585608375604473	29.448684954689135	21.57217952483877
43	20.41062558364863	28.371570995786772	29.63308504061975	21.58471837994485
44	19.987592671346086	29.44894800759346	30.69507345752353	19.868385863536922
45	19.686397095895934	31.5374565414681	28.351798185811806	20.42434817682416
46	21.45319192778321	30.353981524917685	28.534751480768637	19.658075066530465
47	20.483008974488254	29.437899785611883	29.35271448676186	20.726376753138002
48	20.427197916620997	29.407561017313267	30.918054636088225	19.247186429977507
49	21.19995966522134	27.547689299446276	31.021565953939433	20.230785081392952
50	19.405851173435167	30.31724180261387	30.056687900881666	20.220219123069302
51	19.05515780982152	31.40799067039033	29.13758105317614	20.39927046661201
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2159.0
1	3172.5
2	4186.0
3	35249.5
4	66313.0
5	43416.0
6	20519.0
7	20508.0
8	20497.0
9	22150.0
10	23803.0
11	25514.0
12	27225.0
13	27262.0
14	27299.0
15	26140.0
16	24981.0
17	23417.0
18	21853.0
19	20025.0
20	18197.0
21	17287.5
22	16378.0
23	16341.0
24	16304.0
25	17684.5
26	22622.5
27	26180.0
28	29725.5
29	33271.0
30	38403.0
31	43535.0
32	50677.5
33	57820.0
34	63216.5
35	68613.0
36	77487.0
37	86361.0
38	95572.5
39	104784.0
40	120739.5
41	136695.0
42	150280.5
43	163866.0
44	173048.0
45	182230.0
46	186414.0
47	190598.0
48	188043.5
49	185489.0
50	172466.0
51	159443.0
52	145854.5
53	132266.0
54	118120.0
55	103974.0
56	95862.0
57	87750.0
58	77292.0
59	66834.0
60	57329.0
61	47824.0
62	41001.5
63	34179.0
64	28413.0
65	22647.0
66	18117.0
67	13587.0
68	11788.0
69	9989.0
70	7951.5
71	5914.0
72	5043.5
73	4173.0
74	3138.5
75	1607.0
76	1110.0
77	807.0
78	504.0
79	366.5
80	229.0
81	175.0
82	121.0
83	71.5
84	22.0
85	12.5
86	3.0
87	3.0
88	3.0
89	2.5
90	2.0
91	3.5
92	5.0
93	5.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2280910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.47078628401257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51359787965221	19.212894741018715
2	7.330040344373415	3.587437014407034
3	2.871856088572913	2.108297297457241
4	1.6836776598356387	1.648036647400173
5	1.1435139776091456	1.399134307942727
6	0.7974019168188908	1.1707831133362223
7	0.6159014599408905	1.0550115098757402
8	0.48712912259219554	0.953634612133774
9	0.4000331967811996	0.8810214178448772
>10	4.414758093249216	25.146114958092507
>50	1.2298430793323005	21.195925453581278
>100	0.5069887317168792	18.075941871104266
>500	0.0034451910682680308	0.6267081531613267
>1k	0.0016319326112848565	0.7338661954924373
>5k	0.0	0.0
>10k+	1.813258456983174E-4	2.205192707151771
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49698	2.1788672065096826	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT	3469	0.1520884208495732	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT	3160	0.13854119627692457	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTC	2320	0.10171378967166614	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.384215072054575E-5	0.0	0.0	0.04857710299836469	0.0
2	4.384215072054575E-5	0.0	0.0	0.15445589698848267	0.0
3	4.384215072054575E-5	0.0	0.0	0.2543721584806064	0.0
4	4.384215072054575E-5	0.0	0.0	0.4036546816840647	0.0
5	4.384215072054575E-5	0.0	0.0	0.7938059809462013	0.0
6	4.384215072054575E-5	0.0	0.0	1.3668228908637343	0.0
7	8.76843014410915E-5	0.0	0.0	1.8016931838608274	0.0
8	8.76843014410915E-5	0.0	0.0	2.3367866334050884	0.0
9	8.76843014410915E-5	0.0	0.0	2.6428048454344975	0.0
10	8.76843014410915E-5	0.0	0.0	2.9836337251360203	0.0
11	8.76843014410915E-5	0.0	0.0	3.385929300147748	0.0
12	8.76843014410915E-5	0.0	0.0	3.6351719269940506	0.0
13	8.76843014410915E-5	0.0	0.0	3.7520551008150256	0.0
14	8.76843014410915E-5	0.0	0.0	3.812031163000732	0.0
15	8.76843014410915E-5	0.0	0.0	3.8801618652204604	0.0
16	8.76843014410915E-5	0.0	0.0	4.000727779701961	0.0
17	8.76843014410915E-5	0.0	0.0	4.1514570938791975	0.0
18	8.76843014410915E-5	0.0	0.0	4.3262119066512925	0.0
19	8.76843014410915E-5	0.0	0.0	4.455283198372579	0.0
20	8.76843014410915E-5	0.0	0.0	4.583346120627294	0.0
21	8.76843014410915E-5	0.0	0.0	4.751875347997071	0.0
22	8.76843014410915E-5	0.0	0.0	4.9318035345541915	0.0
23	8.76843014410915E-5	0.0	0.0	5.1265942102055755	0.0
24	8.76843014410915E-5	0.0	0.0	5.27635899706696	0.0
25	8.76843014410915E-5	0.0	0.0	5.407490869872112	0.0
26	8.76843014410915E-5	0.0	0.0	5.528100626504334	0.0
27	8.76843014410915E-5	0.0	0.0	5.668702403865124	0.0
28	8.76843014410915E-5	0.0	0.0	5.811101709405457	0.0
29	8.76843014410915E-5	0.0	0.0	5.977438829239207	0.0
30	8.76843014410915E-5	0.0	0.0	6.158682280317943	0.0
31	8.76843014410915E-5	0.0	0.0	6.32072286938108	0.0
32	8.76843014410915E-5	0.0	0.0	6.479913718647382	0.0
33	8.76843014410915E-5	0.0	0.0	6.6357725644589225	0.0
34	8.76843014410915E-5	0.0	0.0	6.802898843005642	0.0
35	8.76843014410915E-5	0.0	0.0	6.997514150054145	0.0
36	8.76843014410915E-5	0.0	0.0	7.171085224756785	0.0
37	8.76843014410915E-5	0.0	0.0	7.353863151110741	0.0
38	8.76843014410915E-5	0.0	0.0	7.572942378261308	0.0
39	8.76843014410915E-5	0.0	0.0	7.911447623974642	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	20	7.034748E-4	45.000004	1
GCGTATA	20	7.034748E-4	45.000004	18
ACGTTAT	35	1.2126293E-7	45.0	41
CCTCGCG	25	3.892018E-5	45.0	34
CGTTTTT	20750	0.0	44.13253	1
TATTGCG	210	0.0	41.785713	1
TATAGCG	200	0.0	41.625	1
ATAACGG	155	0.0	40.64516	2
GATTACG	100	0.0	40.5	1
CACGACC	420	0.0	40.17857	27
TAGGGCG	460	0.0	40.108692	5
TACGGGT	320	0.0	40.07813	4
TGCGTTG	80	0.0	39.375004	1
AAACACG	430	0.0	38.72093	40
TGCGACA	35	6.2509953E-6	38.571426	30
CGGGATC	635	0.0	38.267715	6
ATTGCGG	590	0.0	38.135593	2
AGGGCGC	325	0.0	38.076923	6
ATAGGGC	1095	0.0	38.0137	4
CTAGCGG	160	0.0	37.968754	2
>>END_MODULE