FastQCFastQC Report
Thu 26 May 2016
SRR1547049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547049_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1671999
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77460.4632777890417399No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17320.10358857870130306No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16720.1000000598086482No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGT207.034207E-445.00000422
TAGGTCG502.1827873E-1145.01
CGTAAGA253.8915692E-545.033
CGGTCTA3550.042.4647931
GGCGATA2400.042.1874968
GCGATAT1250.041.49
ACGGGTA2150.040.8139535
CGTTTTT42750.040.4736821
CGACGGT3600.040.028
TAAAGCG800.039.3750041
CTAAGCG800.039.3750041
TCGACGT1800.038.7526
ACGGGTC1350.038.3333325
TAGGGTA8250.038.181825
CGTAAGG1950.038.0769272
TTACGCG659.094947E-1238.076921
CGGGTAC1850.037.70276
CGACGTT1850.037.702727
AGGGTAA8800.037.5852286
GTTAGCG1500.037.51
Produced by FastQC (version 0.11.5)