##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547049_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1671999
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4644458519413	31.0	31.0	33.0	30.0	34.0
2	31.871074683657106	31.0	31.0	34.0	30.0	34.0
3	31.995137556900453	33.0	31.0	34.0	30.0	34.0
4	35.737763599140905	37.0	35.0	37.0	35.0	37.0
5	35.65645254572521	37.0	35.0	37.0	33.0	37.0
6	35.72229110184875	37.0	35.0	37.0	33.0	37.0
7	36.04878890477805	37.0	35.0	37.0	35.0	37.0
8	36.030388774155966	37.0	35.0	37.0	35.0	37.0
9	37.818986733843744	39.0	38.0	39.0	35.0	39.0
10	37.2143948650687	39.0	37.0	39.0	34.0	39.0
11	36.969538857379696	39.0	37.0	39.0	33.0	39.0
12	36.462017022737456	39.0	35.0	39.0	32.0	39.0
13	36.28630698941806	39.0	35.0	39.0	32.0	39.0
14	37.275263920612396	39.0	35.0	41.0	32.0	41.0
15	37.49118151386454	39.0	35.0	41.0	32.0	41.0
16	37.56171803930505	40.0	35.0	41.0	33.0	41.0
17	37.49922757130835	39.0	35.0	41.0	33.0	41.0
18	37.42617011134576	39.0	35.0	41.0	32.0	41.0
19	37.39369880005909	39.0	35.0	41.0	32.0	41.0
20	37.2398883013686	39.0	35.0	41.0	32.0	41.0
21	37.14196240547991	39.0	35.0	41.0	32.0	41.0
22	37.06481164163376	39.0	35.0	41.0	32.0	41.0
23	37.026571188140665	39.0	35.0	41.0	32.0	41.0
24	36.93666443580409	39.0	35.0	41.0	32.0	41.0
25	36.86649035077174	38.0	35.0	41.0	32.0	41.0
26	36.745667910088464	38.0	35.0	41.0	31.0	41.0
27	36.656109842170956	38.0	35.0	41.0	31.0	41.0
28	36.58679042272154	38.0	35.0	40.0	31.0	41.0
29	36.62399140190873	38.0	35.0	41.0	31.0	41.0
30	36.60263014511372	38.0	35.0	41.0	31.0	41.0
31	36.530427948820545	38.0	35.0	41.0	31.0	41.0
32	36.46940219461854	38.0	35.0	41.0	31.0	41.0
33	36.385148555710856	38.0	35.0	41.0	30.0	41.0
34	36.27192001909092	38.0	35.0	41.0	30.0	41.0
35	36.19847021439606	38.0	35.0	41.0	30.0	41.0
36	36.11090198020453	38.0	35.0	41.0	30.0	41.0
37	36.07449286751966	38.0	35.0	41.0	30.0	41.0
38	36.01038756602127	38.0	35.0	41.0	30.0	41.0
39	35.93037555644471	38.0	35.0	41.0	30.0	41.0
40	35.78562487178521	38.0	35.0	40.0	29.0	41.0
41	35.73763979523911	38.0	35.0	40.0	29.0	41.0
42	35.69572230605401	38.0	35.0	40.0	29.0	41.0
43	35.61420670706143	38.0	35.0	40.0	29.0	41.0
44	35.49312350067195	38.0	34.0	40.0	28.0	41.0
45	35.3762615886732	38.0	34.0	40.0	28.0	41.0
46	35.35448944646498	37.0	34.0	40.0	28.0	41.0
47	35.32173942687765	37.0	34.0	40.0	28.0	41.0
48	35.23287633545235	37.0	34.0	40.0	28.0	41.0
49	35.15319088109502	37.0	34.0	40.0	27.0	41.0
50	35.078635812581226	37.0	34.0	40.0	27.0	41.0
51	34.74666073364877	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	6.0
12	7.0
13	12.0
14	29.0
15	43.0
16	107.0
17	258.0
18	558.0
19	1075.0
20	1718.0
21	2899.0
22	4456.0
23	6739.0
24	10035.0
25	14716.0
26	19830.0
27	23886.0
28	26754.0
29	30672.0
30	36817.0
31	45625.0
32	57909.0
33	76872.0
34	132028.0
35	191211.0
36	120305.0
37	159253.0
38	241728.0
39	466085.0
40	362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.57765285744788	23.70294479841196	27.467719777344364	13.251682566795795
2	32.05689716321601	24.667957337295057	29.615388525950078	13.659756973538858
3	29.829862338434417	25.27878306147312	31.219815322856054	13.67153927723641
4	27.14553058943217	26.526929740986688	30.991824755876053	15.335714913705093
5	23.90503822071664	30.795831815688885	29.43835492724577	15.860775036348706
6	23.042238661625994	40.70169898426973	26.073998848085434	10.182063506018842
7	86.9653032089134	4.137263239989976	6.743185851187709	2.1542476999089115
8	86.99855681731867	3.752813249290221	6.94611659456734	2.302513338823767
9	83.0810903595038	5.43511090616681	8.338581542213841	3.145217192115546
10	42.53740582380731	28.77537606182779	16.450847159597583	12.236370954767317
11	36.05295218478001	25.804979548432744	22.477405787922123	15.664662478865118
12	32.7396726911918	22.95288454119889	26.998999401315434	17.30844336629388
13	24.82962011340916	28.562576891493357	28.02956221863769	18.578240776459793
14	18.465680900526856	33.06359632990211	29.54559183348794	18.925130936083097
15	18.32477172534194	27.78817451445844	36.447031367841724	17.44002239235789
16	22.451987112432484	25.058507810112328	35.179147834418565	17.310357243036627
17	22.29612577519484	24.53512232961862	28.06975363023542	25.098998264951117
18	24.02549283821342	27.143676521337635	30.646370003809807	18.184460636639134
19	26.41389139586806	27.60940646495602	26.903903650660077	19.072798488515843
20	28.41042369044479	27.057312833321074	26.23865205661008	18.293611419624053
21	26.201750120663945	26.6212479792153	29.74726659525514	17.429735304865613
22	24.002825360541483	22.331592303583914	29.62627369992446	24.039308635950142
23	21.28302708315017	27.384645564979404	29.548103796712798	21.784223555157627
24	21.085299692164888	26.009106464776593	33.39236446911751	19.513229373941012
25	21.89570687542277	26.737456182689108	30.519635478250883	20.847201463637237
26	20.583804176916374	31.08865495732952	27.130877470620497	21.196663395133612
27	19.863528626512338	30.873104589177387	30.251991777507044	19.011375006803235
28	18.052702184630494	28.79307942169822	33.73668285686774	19.417535536803552
29	19.48212887687134	27.479741315634758	32.89302206520458	20.14510774228932
30	21.31131657375393	27.241403852514267	31.655640942368983	19.79163863136282
31	26.568795794734328	26.399896172186704	27.60880837847391	19.42249965460506
32	25.82800587799395	26.854441898589656	28.517481170742325	18.800071052674074
33	25.489548737768388	26.317719089544912	27.42465755063251	20.7680746220542
34	21.380933840271435	26.85432228129323	28.88931153666958	22.875432341765755
35	20.466040948589086	27.12310234635308	30.122506054130415	22.28835065092742
36	26.273580307165258	26.364070791908368	28.202588637911862	19.159760263014512
37	21.260299796830022	30.043738064436642	28.081715359877606	20.61424677885573
38	22.11951083702801	30.85767395793897	25.735182855970606	21.28763234906241
39	21.856771445437467	28.044992849876106	27.249717254615586	22.84851845007084
40	23.620707907121954	25.91018296063574	28.248940340275325	22.220168791966984
41	20.003540671974086	25.6359603085887	29.531716227103004	24.828782792334206
42	21.443673112244685	26.62459726351511	27.558329879383898	24.373399744856307
43	21.805156582031447	25.325433807077637	28.430399778947233	24.43900983194368
44	21.251986394728707	27.475913562149263	29.587099035346316	21.68500100777572
45	20.682428637816173	30.12334337520537	27.17376027138772	22.020467715590737
46	22.391640186387672	28.60097404364476	27.690327565985385	21.317058203982178
47	21.631412458978744	27.6597055381014	28.782612908261306	21.926269094658547
48	21.951687770148187	27.420889605795217	29.87154896623742	20.755873657819173
49	22.45049189622721	25.87256332091108	30.534647448951823	21.142297333909887
50	20.404557658228264	29.01981400706579	29.256297402091747	21.319330932614193
51	19.909282242393687	29.710005807419744	27.782492692878403	22.59821925730817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1909.0
1	2936.5
2	3964.0
3	8848.5
4	13733.0
5	10059.5
6	6386.0
7	6746.0
8	7106.0
9	7373.0
10	7640.0
11	7393.0
12	7146.0
13	7068.5
14	6991.0
15	6636.5
16	6282.0
17	6186.0
18	6090.0
19	5933.5
20	5777.0
21	6688.5
22	7600.0
23	8462.5
24	9325.0
25	11684.5
26	16481.5
27	18919.0
28	21497.0
29	24075.0
30	27959.5
31	31844.0
32	33987.0
33	36130.0
34	41494.5
35	46859.0
36	51931.0
37	57003.0
38	65431.0
39	73859.0
40	85862.5
41	97866.0
42	112781.0
43	127696.0
44	130854.5
45	134013.0
46	138180.5
47	142348.0
48	141437.0
49	140526.0
50	132900.0
51	125274.0
52	114311.0
53	103348.0
54	94448.5
55	85549.0
56	79771.0
57	73993.0
58	69902.0
59	65811.0
60	59328.5
61	52846.0
62	47007.0
63	41168.0
64	36045.5
65	30923.0
66	25031.0
67	19139.0
68	17158.5
69	15178.0
70	12405.5
71	9633.0
72	8063.5
73	6494.0
74	5312.0
75	2997.5
76	1865.0
77	1278.5
78	692.0
79	579.0
80	466.0
81	342.5
82	219.0
83	138.5
84	58.0
85	41.5
86	25.0
87	20.0
88	15.0
89	9.0
90	3.0
91	8.0
92	13.0
93	11.5
94	10.0
95	12.5
96	15.0
97	8.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1671999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.2335471566069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40930828431961	16.86141292128635
2	7.050085367415206	2.9939664021423007
3	2.211940818268902	1.4090204901700916
4	1.2155576347649795	1.0324240143742296
5	0.7880638371813407	0.8366695324603289
6	0.5609053718554613	0.7146006398212238
7	0.4442614516147237	0.6603272537906709
8	0.36154775181472115	0.6141552990018472
9	0.31287376713003884	0.5979077899578833
>10	5.220068621827096	28.247333600912906
>50	1.7714731451829377	26.636837682182247
>100	0.6468246820535977	17.728492521136072
>500	0.005671413257159832	0.8517605140049188
>1k	0.0011342826514319664	0.34868833144369327
>5k	2.835706628579916E-4	0.4664030073152907
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7746	0.4632777890417399	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1732	0.10358857870130306	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1672	0.1000000598086482	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.19617296421828E-4	0.0	0.0	0.0666268341069582	0.0
2	1.19617296421828E-4	0.0	0.0	0.19324174236946315	0.0
3	1.19617296421828E-4	0.0	0.0	0.27272743584176784	0.0
4	1.19617296421828E-4	0.0	0.0	0.3965911462865708	0.0
5	1.19617296421828E-4	0.0	0.0	0.7092109504850183	0.0
6	1.19617296421828E-4	0.0	0.0	0.9974288262134128	0.0
7	1.19617296421828E-4	0.0	0.0	1.1779911351621621	0.0
8	1.19617296421828E-4	0.0	0.0	1.5495822664965708	0.0
9	1.19617296421828E-4	0.0	0.0	1.6880392871048369	0.0
10	1.19617296421828E-4	0.0	0.0	1.9474293943955707	0.0
11	1.19617296421828E-4	0.0	0.0	2.3311616813167952	0.0
12	1.19617296421828E-4	0.0	0.0	2.6233867364753207	0.0
13	1.19617296421828E-4	0.0	0.0	2.7480877679950764	0.0
14	1.19617296421828E-4	0.0	0.0	2.805504070277554	0.0
15	1.19617296421828E-4	0.0	0.0	2.8948581907046593	0.0
16	1.19617296421828E-4	0.0	0.0	3.068363079164521	0.0
17	1.19617296421828E-4	0.0	0.0	3.2571789815663768	0.0
18	1.19617296421828E-4	0.0	0.0	3.495456636038658	0.0
19	1.19617296421828E-4	0.0	0.0	3.6369040890574693	0.0
20	1.19617296421828E-4	0.0	0.0	3.792466383054057	0.0
21	1.19617296421828E-4	0.0	0.0	3.9909712864660802	0.0
22	1.79425944632742E-4	0.0	0.0	4.18792116502462	0.0
23	1.79425944632742E-4	0.0	0.0	4.393064828388055	0.0
24	1.79425944632742E-4	0.0	0.0	4.558794592580498	0.0
25	1.79425944632742E-4	0.0	0.0	4.699703767765412	0.0
26	4.1866053747639803E-4	0.0	0.0	4.838758874855786	0.0
27	4.1866053747639803E-4	0.0	0.0	4.9966537061326	0.0
28	4.1866053747639803E-4	0.0	0.0	5.155445667132576	0.0
29	4.1866053747639803E-4	0.0	0.0	5.342586927384526	0.0
30	4.1866053747639803E-4	0.0	0.0	5.542168386464346	0.0
31	4.1866053747639803E-4	0.0	0.0	5.720099114891815	0.0
32	4.1866053747639803E-4	0.0	0.0	5.892706873628513	0.0
33	4.1866053747639803E-4	0.0	0.0	6.069680663684608	0.0
34	4.1866053747639803E-4	0.0	0.0	6.245757324017538	0.0
35	4.1866053747639803E-4	0.0	0.0	6.461666544058938	0.0
36	4.1866053747639803E-4	0.0	0.0	6.651917854017856	0.0
37	4.1866053747639803E-4	0.0	0.0	6.852934720654737	0.0
38	4.1866053747639803E-4	0.0	0.0	7.077217151445665	0.0
39	4.1866053747639803E-4	0.0	0.0	7.426140805108137	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTACGT	20	7.034207E-4	45.000004	22
TAGGTCG	50	2.1827873E-11	45.0	1
CGTAAGA	25	3.8915692E-5	45.0	33
CGGTCTA	355	0.0	42.46479	31
GGCGATA	240	0.0	42.187496	8
GCGATAT	125	0.0	41.4	9
ACGGGTA	215	0.0	40.813953	5
CGTTTTT	4275	0.0	40.473682	1
CGACGGT	360	0.0	40.0	28
TAAAGCG	80	0.0	39.375004	1
CTAAGCG	80	0.0	39.375004	1
TCGACGT	180	0.0	38.75	26
ACGGGTC	135	0.0	38.333332	5
TAGGGTA	825	0.0	38.18182	5
CGTAAGG	195	0.0	38.076927	2
TTACGCG	65	9.094947E-12	38.07692	1
CGGGTAC	185	0.0	37.7027	6
CGACGTT	185	0.0	37.7027	27
AGGGTAA	880	0.0	37.585228	6
GTTAGCG	150	0.0	37.5	1
>>END_MODULE