FastQCFastQC Report
Thu 26 May 2016
SRR1547048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547048_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences940187
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40050.4259790871390478No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG38910.4138538397148652No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG32950.3504621952866824No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22020.23420872656184352No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA11750.12497513792468944No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA10600.11274352868099645No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9810.10434094493967688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG207.03263E-445.00000443
ACTATCG207.03263E-445.0000041
GATACCA351.2116107E-745.00000420
GGTACGA207.03263E-445.0000049
GTCGATA207.03263E-445.00000425
CAACGCG207.03263E-445.00000414
TAACGTA207.03263E-445.00000434
GTTACAT207.03263E-445.0000048
CTAACGT207.03263E-445.00000433
CATAGGT302.1650776E-645.00000435
CTACGGC207.03263E-445.00000441
ACTACGG207.03263E-445.00000440
CGAATAT302.1650776E-645.00000414
GATGCGA406.8121153E-945.0000049
CATACGC351.2116107E-745.00000432
ACAGACG207.03263E-445.00000411
CCAACGG302.1650776E-645.0000042
CACGTGC207.03263E-445.00000442
CACAACG207.03263E-445.00000412
CGATCAA207.03263E-445.00000410