FastQCFastQC Report
Thu 26 May 2016
SRR1547047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547047_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1577175
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65930.4180259007402476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT207.034087E-445.014
CTATCGA207.034087E-445.015
TCGTACA551.8189894E-1245.034
GTAAGCG453.8562575E-1045.01
CGCTTAT207.034087E-445.043
CACGACG3450.043.69565226
CGGTCTA3450.043.0434831
TAGTACG1100.042.9545441
TCACGAC3550.042.4647925
GCGTAAG603.6379788E-1241.2500041
CGTAAAC556.184564E-1140.90909239
CGTTTTT38700.040.872091
GCGGGTC1000.040.5000045
TAGACGG950.040.2631572
CTCCACG1750.039.85714326
TTCGCGG1250.039.6000022
ACGACGG3750.039.60000227
TTACACG2900.039.5689734
TAATGCG403.4593177E-739.3751
TACCGCG403.4593177E-739.3751