##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547047_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1577175
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.434604910678903	31.0	31.0	33.0	30.0	34.0
2	31.839658249718642	31.0	31.0	34.0	30.0	34.0
3	31.968839222026723	33.0	31.0	34.0	30.0	34.0
4	35.70698051896587	37.0	35.0	37.0	33.0	37.0
5	35.61261052197759	37.0	35.0	37.0	33.0	37.0
6	35.67872430136161	37.0	35.0	37.0	33.0	37.0
7	36.00661879626548	37.0	35.0	37.0	35.0	37.0
8	35.991626801084216	37.0	35.0	37.0	35.0	37.0
9	37.76419103777324	39.0	38.0	39.0	35.0	39.0
10	37.205719086341084	39.0	37.0	39.0	33.0	39.0
11	36.911541839047665	39.0	37.0	39.0	33.0	39.0
12	36.33036093014409	38.0	35.0	39.0	32.0	39.0
13	36.11728311696546	38.0	35.0	39.0	32.0	39.0
14	37.06681186298287	39.0	35.0	41.0	31.0	41.0
15	37.32704424049329	39.0	35.0	41.0	32.0	41.0
16	37.42873999397657	39.0	35.0	41.0	32.0	41.0
17	37.37958057920015	39.0	35.0	41.0	32.0	41.0
18	37.27168640131882	39.0	35.0	41.0	32.0	41.0
19	37.230561605402066	39.0	35.0	41.0	32.0	41.0
20	37.086291629020245	39.0	35.0	41.0	32.0	41.0
21	36.9960207332731	39.0	35.0	41.0	32.0	41.0
22	36.92736126301774	39.0	35.0	41.0	32.0	41.0
23	36.90536053386593	38.0	35.0	41.0	32.0	41.0
24	36.8246301139696	38.0	35.0	41.0	31.0	41.0
25	36.76267630415141	38.0	35.0	41.0	31.0	41.0
26	36.63398164439583	38.0	35.0	41.0	31.0	41.0
27	36.55028769794094	38.0	35.0	41.0	31.0	41.0
28	36.46559766671422	38.0	35.0	40.0	31.0	41.0
29	36.51432466276729	38.0	35.0	41.0	31.0	41.0
30	36.46616006467259	38.0	35.0	40.0	31.0	41.0
31	36.36758444687495	38.0	35.0	41.0	30.0	41.0
32	36.31170795885048	38.0	35.0	41.0	30.0	41.0
33	36.215731925753325	38.0	35.0	41.0	30.0	41.0
34	36.10213704883732	38.0	35.0	41.0	30.0	41.0
35	36.03707958850476	38.0	35.0	41.0	30.0	41.0
36	35.939297795108345	38.0	35.0	41.0	29.0	41.0
37	35.92623836923613	38.0	35.0	41.0	29.0	41.0
38	35.84701887869133	38.0	35.0	41.0	29.0	41.0
39	35.78299808201373	38.0	35.0	41.0	29.0	41.0
40	35.6635928162696	38.0	35.0	40.0	29.0	41.0
41	35.62327325756495	38.0	35.0	40.0	29.0	41.0
42	35.58294165200437	38.0	35.0	40.0	28.0	41.0
43	35.50346156894447	38.0	34.0	40.0	28.0	41.0
44	35.383202244519474	38.0	34.0	40.0	27.0	41.0
45	35.28719767939512	38.0	34.0	40.0	27.0	41.0
46	35.27546721194541	37.0	34.0	40.0	27.0	41.0
47	35.25671723175932	37.0	34.0	40.0	27.0	41.0
48	35.16764404710955	37.0	34.0	40.0	27.0	41.0
49	35.101892941493496	37.0	34.0	40.0	27.0	41.0
50	35.005359582798356	37.0	34.0	40.0	27.0	41.0
51	34.645353242347866	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	6.0
12	16.0
13	10.0
14	20.0
15	47.0
16	104.0
17	250.0
18	610.0
19	1168.0
20	2069.0
21	3297.0
22	4880.0
23	7409.0
24	10634.0
25	15336.0
26	20531.0
27	24242.0
28	26856.0
29	30236.0
30	36143.0
31	43946.0
32	55481.0
33	72501.0
34	126517.0
35	187351.0
36	104891.0
37	139186.0
38	218285.0
39	444844.0
40	302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.19940716787928	22.901580357284384	27.9303818536307	12.968630621205637
2	32.67145370678587	24.90218270008084	28.991900074500293	13.434463518632999
3	30.46453310507712	24.878596224261734	30.77496156101891	13.88190910964224
4	27.474820485995533	27.132626373103808	29.886347424984542	15.506205715916115
5	24.767194509169876	30.98673260735175	29.2173030893845	15.028769794093872
6	23.549637801765815	40.29102667744544	25.813812671390302	10.345522849398451
7	86.45908031765657	4.457590311791653	6.944695420609634	2.1386339499421436
8	87.50836146908237	3.6068920696815505	6.71301535974131	2.1717311014947613
9	82.43619129139125	5.88146527810801	8.3416868768526	3.3406565536481367
10	45.2192052245312	30.682898219918524	14.234818583860385	9.863077971689888
11	36.62009605782491	24.783172444402172	24.12287793047696	14.473853567295954
12	34.335504937625814	23.153359646202862	26.773249639386876	15.737885776784442
13	25.42875711319289	30.59127871035237	26.646757652131186	17.33320652432355
14	17.043447936975923	33.59405265744131	30.496742593561272	18.865756812021495
15	16.64748680393742	28.424619969248816	38.72239922646504	16.205494000348725
16	22.74795124193574	25.267963288791666	35.76280374720624	16.22128172206635
17	22.7041387290567	24.292421576553014	28.205494000348725	24.797945694041562
18	23.821199296209997	26.585255282387816	31.59750820295782	17.99603721844437
19	26.75657425460079	27.671247642144976	26.90646250416092	18.665715599093318
20	28.51446415267805	27.603404821912598	26.96954998652654	16.912581038882813
21	25.499896967679554	26.57580801115919	29.965603056097134	17.95869196506412
22	23.67657362055574	23.406724047743594	30.60776388162379	22.30893845007688
23	20.443514511706056	27.462710225561526	30.712191101177737	21.381584161554677
24	21.149460269152122	25.720037408657888	34.905511436587574	18.22499088560242
25	21.280834403284356	27.380791605243555	31.687923026931063	19.65045096454103
26	19.431832231680062	33.28584335917067	27.09800751343383	20.18431689571544
27	18.672373072106772	32.425254014297714	30.254854407405645	18.647518506189865
28	16.873967695404758	29.181796566646064	35.03523705359266	18.908998684356522
29	18.885539017547195	27.766037376955634	33.422860494238115	19.925563111259052
30	20.760093204622187	29.24329893638943	31.50962955918018	18.4869782998082
31	25.665509534452426	27.027565108500962	28.80796360581419	18.498961751232425
32	27.048488595114684	27.532391776435716	27.639481985195047	17.779637643254553
33	25.99369125176344	27.69496092697386	27.287523578550253	19.023824242712443
34	20.50634837605212	28.05085041292184	29.784075958596855	21.658725252429186
35	20.803652099481667	28.670946470746745	30.364607605370363	20.160793824401225
36	26.354272671073282	28.179561557848686	27.42916924247468	18.036996528603357
37	21.898521089923438	31.528080270103192	27.64969011048235	18.92370852949102
38	21.227130787642462	32.028405218190755	25.928955252270676	20.81550874189611
39	21.26942159240414	30.262336138982675	27.549003756716917	20.91923851189627
40	23.78949704376496	26.96695040182605	27.78201531218793	21.46153724222106
41	19.433417344302313	26.2953698860304	29.129678063626418	25.141534706040865
42	21.19961323252017	26.981089606416536	27.20541474471761	24.61388241634568
43	21.533818377795743	26.936579643983706	27.920807773392298	23.608794204828254
44	20.425127205287936	28.56943585841774	29.52158130835196	21.483855627942365
45	19.87712206952304	32.60640068476866	26.575110561605403	20.941366684102906
46	21.868403950100653	30.96530188469891	27.15995371471143	20.006340450489006
47	20.86718341338152	29.12111845546626	28.004882146876536	22.006815984275683
48	20.766877486645427	27.50899551413128	31.16293372644126	20.56119327278203
49	22.23767178658044	27.447873571417247	29.90841219268629	20.406042449316026
50	20.01033493429708	30.769445369093475	28.608302819915355	20.61191687669409
51	19.67505191243838	31.175487818409497	27.596747348899136	21.552712920252983
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1248.0
1	2595.5
2	3943.0
3	8299.0
4	12655.0
5	9346.0
6	6037.0
7	6044.0
8	6051.0
9	6125.0
10	6199.0
11	6111.5
12	6024.0
13	6085.5
14	6147.0
15	5984.5
16	5822.0
17	5828.5
18	5835.0
19	5846.0
20	5857.0
21	6920.5
22	7984.0
23	9443.5
24	10903.0
25	12114.5
26	14953.5
27	16581.0
28	20511.5
29	24442.0
30	28193.5
31	31945.0
32	40287.5
33	48630.0
34	50534.5
35	52439.0
36	56911.0
37	61383.0
38	68606.5
39	75830.0
40	90417.5
41	105005.0
42	120404.0
43	135803.0
44	137121.0
45	138439.0
46	134542.0
47	130645.0
48	125095.5
49	119546.0
50	111572.5
51	103599.0
52	97892.5
53	92186.0
54	85304.0
55	78422.0
56	71673.5
57	64925.0
58	61611.5
59	58298.0
60	51632.5
61	44967.0
62	38418.5
63	31870.0
64	27417.5
65	22965.0
66	19337.5
67	15710.0
68	12894.0
69	10078.0
70	8298.0
71	6518.0
72	5406.0
73	4294.0
74	3448.5
75	1876.5
76	1150.0
77	847.5
78	545.0
79	367.5
80	190.0
81	134.5
82	79.0
83	49.0
84	19.0
85	21.0
86	23.0
87	15.5
88	8.0
89	6.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1577175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.868753410323905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.13541165591305	15.309240753805433
2	6.22311720973206	2.348449281479544
3	2.0186860050053475	1.1427026532395337
4	1.062904227076886	0.8022271103801875
5	0.6522729352808229	0.6153788586021005
6	0.5005943792738308	0.5667355140667243
7	0.3801475566369571	0.5021037354003919
8	0.3345522516078219	0.5050067150765305
9	0.29380899818828515	0.49894285828941504
>10	4.403569758273906	20.88607241551997
>50	1.7910023331082845	24.16978732982348
>100	1.198854233674371	31.60324798427822
>500	0.004401328731365851	0.5647874710262826
>1k	3.3856374856660394E-4	0.06413828988919666
>5k	3.3856374856660394E-4	0.4211790291229816
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6593	0.4180259007402476	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2680900978014487E-4	0.0	0.0	0.02238179022619557	0.0
2	1.2680900978014487E-4	0.0	0.0	0.06422876345364338	0.0
3	1.2680900978014487E-4	0.0	0.0	0.09434590327642779	0.0
4	1.2680900978014487E-4	0.0	0.0	0.1518537892117235	0.0
5	1.2680900978014487E-4	0.0	0.0	0.27517555122291437	0.0
6	1.2680900978014487E-4	0.0	0.0	0.3952002789798215	0.0
7	1.2680900978014487E-4	0.0	0.0	0.47312441548972056	0.0
8	1.2680900978014487E-4	0.0	0.0	0.624027137128093	0.0
9	1.2680900978014487E-4	0.0	0.0	0.6772235167308637	0.0
10	1.2680900978014487E-4	0.0	0.0	0.7751200722811356	0.0
11	1.2680900978014487E-4	0.0	0.0	0.9281151425808803	0.0
12	1.2680900978014487E-4	0.0	0.0	1.0554313884001458	0.0
13	1.2680900978014487E-4	0.0	0.0	1.1053307337486329	0.0
14	1.2680900978014487E-4	0.0	0.0	1.1280295464992787	0.0
15	1.2680900978014487E-4	0.0	0.0	1.1665794854724427	0.0
16	1.2680900978014487E-4	0.0	0.0	1.2391776435715758	0.0
17	1.2680900978014487E-4	0.0	0.0	1.3223009494824607	0.0
18	1.2680900978014487E-4	0.0	0.0	1.4288839222026726	0.0
19	1.2680900978014487E-4	0.0	0.0	1.5038914514876283	0.0
20	1.2680900978014487E-4	0.0	0.0	1.5893607240794458	0.0
21	1.2680900978014487E-4	0.0	0.0	1.6937879436333951	0.0
22	1.2680900978014487E-4	0.0	0.0	1.8065845578328341	0.0
23	1.2680900978014487E-4	0.0	0.0	1.9157671152535387	0.0
24	1.2680900978014487E-4	0.0	0.0	2.0013631968551366	0.0
25	1.2680900978014487E-4	0.0	0.0	2.081379682026408	0.0
26	1.2680900978014487E-4	0.0	0.0	2.165137032986194	0.0
27	1.2680900978014487E-4	0.0	0.0	2.2543471713665255	0.0
28	1.2680900978014487E-4	0.0	0.0	2.352053513402127	0.0
29	1.2680900978014487E-4	0.0	0.0	2.4670692852727187	0.0
30	1.2680900978014487E-4	0.0	0.0	2.610997511373183	0.0
31	1.2680900978014487E-4	0.0	0.0	2.7303247895762994	0.0
32	1.2680900978014487E-4	0.0	0.0	2.832342637944426	0.0
33	1.902135146702173E-4	0.0	0.0	2.948436286398149	0.0
34	1.902135146702173E-4	0.0	0.0	3.078288712413017	0.0
35	2.5361801956028975E-4	0.0	0.0	3.2200611853472187	0.0
36	2.5361801956028975E-4	0.0	0.0	3.3468701951273636	0.0
37	2.5361801956028975E-4	0.0	0.0	3.4873111734588744	0.0
38	2.5361801956028975E-4	6.340450489007244E-5	0.0	3.6874157908919427	0.0
39	2.5361801956028975E-4	6.340450489007244E-5	0.0	4.071710495030672	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGT	20	7.034087E-4	45.0	14
CTATCGA	20	7.034087E-4	45.0	15
TCGTACA	55	1.8189894E-12	45.0	34
GTAAGCG	45	3.8562575E-10	45.0	1
CGCTTAT	20	7.034087E-4	45.0	43
CACGACG	345	0.0	43.695652	26
CGGTCTA	345	0.0	43.04348	31
TAGTACG	110	0.0	42.954544	1
TCACGAC	355	0.0	42.46479	25
GCGTAAG	60	3.6379788E-12	41.250004	1
CGTAAAC	55	6.184564E-11	40.909092	39
CGTTTTT	3870	0.0	40.87209	1
GCGGGTC	100	0.0	40.500004	5
TAGACGG	95	0.0	40.263157	2
CTCCACG	175	0.0	39.857143	26
TTCGCGG	125	0.0	39.600002	2
ACGACGG	375	0.0	39.600002	27
TTACACG	290	0.0	39.56897	34
TAATGCG	40	3.4593177E-7	39.375	1
TACCGCG	40	3.4593177E-7	39.375	1
>>END_MODULE