FastQCFastQC Report
Thu 26 May 2016
SRR1547046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547046_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences913880
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.42937803650369855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC253.8901755E-545.00000412
TACGCGC253.8901755E-545.00000415
ATATACG253.8901755E-545.0000041
GACGTAA207.032527E-445.035
CGTTAGA207.032527E-445.013
ATATCGG207.032527E-445.02
GCCGAAT207.032527E-445.045
ACTACGG207.032527E-445.02
ATGACGC207.032527E-445.036
GCGATAT406.8121153E-945.09
TATAGCG351.2115561E-745.01
CGCAATA207.032527E-445.025
TCGACCC207.032527E-445.040
TATCGTG351.2115561E-745.01
CCCGCTT207.032527E-445.044
AATACGG1150.043.043482
CGTTTTT24150.041.925471
CGACGGT1750.041.14285728
CGGTCTA1650.040.90909231
ACGGTCT1650.040.90909230