##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547046_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	913880
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.449644373440716	31.0	31.0	33.0	30.0	34.0
2	31.869882260252986	31.0	31.0	34.0	30.0	34.0
3	31.998013962445835	33.0	31.0	34.0	30.0	34.0
4	35.73843393005646	37.0	35.0	37.0	35.0	37.0
5	35.64350242920296	37.0	35.0	37.0	33.0	37.0
6	35.698279861688626	37.0	35.0	37.0	33.0	37.0
7	36.024051297763386	37.0	35.0	37.0	35.0	37.0
8	36.01539151748588	37.0	35.0	37.0	35.0	37.0
9	37.80976605243577	39.0	38.0	39.0	35.0	39.0
10	37.23796012605594	39.0	37.0	39.0	34.0	39.0
11	36.93152602092179	39.0	37.0	39.0	33.0	39.0
12	36.249394887731434	38.0	35.0	39.0	32.0	39.0
13	35.982620256488815	38.0	35.0	39.0	32.0	39.0
14	36.911573729592504	39.0	35.0	41.0	31.0	41.0
15	37.202097649582	39.0	35.0	41.0	32.0	41.0
16	37.32209371033396	39.0	35.0	41.0	32.0	41.0
17	37.28800608394975	39.0	35.0	41.0	32.0	41.0
18	37.193710333960695	39.0	35.0	41.0	32.0	41.0
19	37.16706788637458	39.0	35.0	41.0	32.0	41.0
20	36.99798113537882	39.0	35.0	41.0	32.0	41.0
21	36.90157569921653	38.0	35.0	41.0	32.0	41.0
22	36.82786580295006	38.0	35.0	41.0	32.0	41.0
23	36.78882128944719	38.0	35.0	41.0	32.0	41.0
24	36.71107475817394	38.0	35.0	41.0	31.0	41.0
25	36.64847572985512	38.0	35.0	41.0	31.0	41.0
26	36.52458637895566	38.0	35.0	40.0	31.0	41.0
27	36.41996761062722	38.0	35.0	40.0	31.0	41.0
28	36.36251696065129	38.0	35.0	40.0	31.0	41.0
29	36.4226101895216	38.0	35.0	40.0	31.0	41.0
30	36.38945594607607	38.0	35.0	40.0	31.0	41.0
31	36.2930330021447	38.0	35.0	40.0	31.0	41.0
32	36.23061889963672	38.0	35.0	41.0	30.0	41.0
33	36.12027618505712	38.0	35.0	41.0	30.0	41.0
34	35.99654440407931	38.0	35.0	41.0	30.0	41.0
35	35.922450431128816	38.0	35.0	41.0	30.0	41.0
36	35.82518492581083	38.0	35.0	40.0	30.0	41.0
37	35.79884995841905	38.0	35.0	40.0	29.0	41.0
38	35.73258305247954	38.0	35.0	40.0	29.0	41.0
39	35.66083730905589	38.0	35.0	40.0	29.0	41.0
40	35.519558366525146	38.0	35.0	40.0	29.0	41.0
41	35.476134722283014	38.0	34.0	40.0	29.0	41.0
42	35.451406092703635	37.0	34.0	40.0	28.0	41.0
43	35.370445791570006	37.0	34.0	40.0	28.0	41.0
44	35.236307830349716	37.0	34.0	40.0	28.0	41.0
45	35.137647174683764	37.0	34.0	40.0	27.0	41.0
46	35.144057206635445	37.0	34.0	40.0	27.0	41.0
47	35.1320665732919	37.0	34.0	40.0	28.0	41.0
48	35.06560817612816	36.0	34.0	40.0	27.0	41.0
49	35.003456690156256	36.0	34.0	40.0	27.0	41.0
50	34.9230052085613	36.0	34.0	40.0	27.0	41.0
51	34.55371164704338	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	4.0
13	6.0
14	9.0
15	21.0
16	66.0
17	142.0
18	307.0
19	645.0
20	1057.0
21	1766.0
22	2777.0
23	4162.0
24	6006.0
25	8624.0
26	11670.0
27	14034.0
28	15565.0
29	17546.0
30	21043.0
31	26112.0
32	32792.0
33	43393.0
34	77594.0
35	118688.0
36	62574.0
37	82986.0
38	124588.0
39	239536.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.60123867466188	23.79951415940824	29.535825272464656	13.063421893465224
2	31.68665470302447	24.73508556922134	29.88379218278111	13.694467544973083
3	29.310631592769294	24.966297544535387	32.24591850133497	13.477152361360353
4	27.690178141550316	27.0739046701974	30.267978290366347	14.967938897885938
5	24.329124173852147	30.87823346610058	29.61417253906421	15.178469820983063
6	22.395391079791658	40.92506674836959	26.078478574867596	10.601063596971155
7	87.3802906289666	4.042106184619425	6.608963977765134	1.968639208648838
8	88.8350768153368	2.891517485884361	6.356414408893947	1.9169912898848864
9	84.25241826060315	4.937737996235829	7.798398039129864	3.0114457040311637
10	47.96701974000963	26.72167024116952	14.19540858756073	11.115901431260122
11	40.15187989670416	23.808705738171312	21.374359872193285	14.665054492931237
12	36.315489998686914	21.698034752921608	25.879108854554207	16.107366393837268
13	24.99037072701011	30.224974832581957	27.513568521031207	17.271085919376723
14	17.649582002013396	33.91440889394669	30.267759443252945	18.168249660786977
15	17.483148772267693	26.063268700485843	40.397316934389636	16.056265592856832
16	22.10519980741454	22.873243751914913	37.93441152011205	17.087144920558497
17	22.870070468770518	22.664354182168335	27.441239550050334	27.024335799010814
18	24.590427627259594	25.746159233159716	30.930100231977942	18.73331290760275
19	27.68350330459141	26.43673129951416	26.689937409725566	19.18982798616886
20	29.501028581433008	26.4546767628135	25.964787499452886	18.079507156300608
21	26.613559767146672	25.697137479756645	29.69777213638552	17.991530616711167
22	24.685626121591454	21.350724383945376	30.657197881559945	23.306451612903224
23	21.51704819013437	26.103098875125834	30.13918676412658	22.24066617061321
24	21.456536963277454	25.12758786711603	34.60257364205366	18.813301527552852
25	21.13253381187902	26.349848995491747	32.13791745086883	20.379699741760408
26	20.361754278461067	32.354028975357814	26.732722020396547	20.551494725784565
27	19.454304722720707	31.186041931106928	30.207138792839324	19.15251455333304
28	17.420886768503525	28.291022891408062	34.876679651595396	19.41141068849302
29	19.579704118702672	26.69343896354007	33.844706088326696	19.882150829430557
30	21.151354663631984	27.745546461242178	31.76861294699523	19.334485928130608
31	27.67715673830262	25.485840591762592	27.273821508294304	19.56318116164048
32	28.66229701930231	25.81826935702718	27.401628222523744	18.117805401146757
33	26.847179060708186	26.4447192191535	26.799032695758747	19.90906902437957
34	21.487284982711078	26.92946557534906	28.809581126624938	22.77366831531492
35	21.15212062852891	27.677813279642844	29.91640040267869	21.25366568914956
36	27.706810522169214	26.020046395588043	27.42471659298814	18.848426489254607
37	22.182343414890358	30.890051210224538	26.967544973081804	19.9600604018033
38	22.516413533505496	31.525802074670633	24.759158751696063	21.19862564012781
39	21.694204928436996	28.92666433229746	27.240556747056505	22.138573992209043
40	24.23403510307699	25.524138836608746	27.80277498139799	22.43905107891627
41	19.977021053092308	24.978443559329452	28.97207510832932	26.072460279248915
42	22.20007003107629	25.913796122029147	26.68982798616886	25.196305860725698
43	21.457740622401193	25.926926948833547	27.625399395981965	24.9899330327833
44	21.093688449249353	27.497592681752526	29.734100757211014	21.674618111787105
45	21.23298463693264	31.34054799317197	25.607191316146537	21.81927605374885
46	22.367269225718914	29.293561517923578	27.492012080360663	20.84715717599685
47	21.935265023854335	27.105199807414543	28.294196174552454	22.665338994178665
48	22.06558847988795	26.754935002407322	30.073751477218014	21.105725040486718
49	21.97465750426752	25.38484264892546	31.08296494069243	21.55753490611459
50	20.69308880815862	29.73464787499453	28.171641791044777	21.400621525802073
51	20.20473147459185	31.119074714404515	27.25401584453101	21.42217796647262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	508.0
1	1284.0
2	2060.0
3	4753.0
4	7446.0
5	5375.5
6	3305.0
7	3321.5
8	3338.0
9	3476.0
10	3614.0
11	3642.0
12	3670.0
13	3425.5
14	3181.0
15	3035.5
16	2890.0
17	2894.5
18	2899.0
19	3040.5
20	3182.0
21	3128.0
22	3074.0
23	3945.5
24	4817.0
25	5635.0
26	7749.5
27	9046.0
28	10782.5
29	12519.0
30	13752.0
31	14985.0
32	17553.0
33	20121.0
34	23365.0
35	26609.0
36	28445.0
37	30281.0
38	34026.5
39	37772.0
40	47235.0
41	56698.0
42	66545.0
43	76392.0
44	78329.0
45	80266.0
46	79183.0
47	78100.0
48	74038.5
49	69977.0
50	68771.0
51	67565.0
52	61066.5
53	54568.0
54	50851.5
55	47135.0
56	43755.0
57	40375.0
58	38562.0
59	36749.0
60	32974.5
61	29200.0
62	26842.0
63	24484.0
64	20800.5
65	17117.0
66	14785.0
67	12453.0
68	10778.0
69	9103.0
70	7208.5
71	5314.0
72	4263.5
73	3213.0
74	2515.5
75	1320.0
76	822.0
77	608.5
78	395.0
79	336.5
80	278.0
81	164.5
82	51.0
83	33.0
84	15.0
85	12.5
86	10.0
87	7.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	913880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.24046677448105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.83830774625498	16.74562456240641
2	5.81531272599751	2.4703991347953385
3	2.0369527315894436	1.2979748044954196
4	1.1700306218943575	0.9940798619788999
5	0.8317114745420191	0.8832969970482197
6	0.6551932663474868	0.8349966482830503
7	0.5614349503277349	0.8347598285928071
8	0.5016427303260507	0.8524100596920348
9	0.4143454780379273	0.7920802223478955
>10	6.929916691586955	36.036052682724495
>50	1.907963845338572	28.44657139902725
>100	0.33459398592803397	9.05599488987745
>500	0.0020750014631195908	0.3233929723173456
>1k	5.187503657798977E-4	0.43236593641337795
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3924	0.42937803650369855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0942355670328708E-4	0.0	0.0	0.028450124742854643	0.0
2	1.0942355670328708E-4	0.0	0.0	0.09607388278548606	0.0
3	1.0942355670328708E-4	0.0	0.0	0.14093754103383377	0.0
4	1.0942355670328708E-4	0.0	0.0	0.21633037160239857	0.0
5	1.0942355670328708E-4	0.0	0.0	0.38484264892546066	0.0
6	1.0942355670328708E-4	0.0	0.0	0.5404429465575349	0.0
7	1.0942355670328708E-4	0.0	0.0	0.6416597365080755	0.0
8	1.0942355670328708E-4	0.0	0.0	0.8375279030069593	0.0
9	1.0942355670328708E-4	0.0	0.0	0.9079966735238763	0.0
10	1.0942355670328708E-4	0.0	0.0	1.0315358690418874	0.0
11	1.0942355670328708E-4	0.0	0.0	1.2209480456952773	0.0
12	1.0942355670328708E-4	0.0	0.0	1.362651551626034	0.0
13	1.0942355670328708E-4	0.0	0.0	1.4324637808027312	0.0
14	1.0942355670328708E-4	0.0	0.0	1.4601479406486628	0.0
15	1.0942355670328708E-4	0.0	0.0	1.5056681402372303	0.0
16	1.0942355670328708E-4	0.0	0.0	1.591346785135904	0.0
17	1.0942355670328708E-4	0.0	0.0	1.6970499409112794	0.0
18	1.0942355670328708E-4	0.0	0.0	1.8347047752440144	0.0
19	1.0942355670328708E-4	0.0	0.0	1.9242132446273035	0.0
20	1.0942355670328708E-4	0.0	0.0	2.0271808114850964	0.0
21	1.0942355670328708E-4	0.0	0.0	2.156081761281569	0.0
22	1.0942355670328708E-4	0.0	0.0	2.2908915831400183	0.0
23	1.0942355670328708E-4	0.0	0.0	2.4228563925241824	0.0
24	1.0942355670328708E-4	0.0	0.0	2.5302009016501072	0.0
25	1.0942355670328708E-4	0.0	0.0	2.629995185363505	0.0
26	1.0942355670328708E-4	0.0	0.0	2.7306648575305292	0.0
27	1.0942355670328708E-4	0.0	0.0	2.8444653565019475	0.0
28	1.0942355670328708E-4	0.0	0.0	2.9651595395456734	0.0
29	1.0942355670328708E-4	0.0	0.0	3.0986562787236838	0.0
30	1.0942355670328708E-4	0.0	0.0	3.2616973782115815	0.0
31	1.0942355670328708E-4	0.0	0.0	3.3981485534205804	0.0
32	1.0942355670328708E-4	0.0	0.0	3.5250798791963933	0.0
33	1.0942355670328708E-4	0.0	0.0	3.6551844881166016	0.0
34	1.0942355670328708E-4	0.0	0.0	3.797435111830875	0.0
35	1.0942355670328708E-4	0.0	0.0	3.9657285420405306	0.0
36	1.0942355670328708E-4	0.0	0.0	4.10217971724953	0.0
37	1.0942355670328708E-4	0.0	0.0	4.2516522957062195	0.0
38	1.0942355670328708E-4	0.0	0.0	4.4603230183393885	0.0
39	1.0942355670328708E-4	0.0	0.0	4.797785267212325	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGC	25	3.8901755E-5	45.000004	12
TACGCGC	25	3.8901755E-5	45.000004	15
ATATACG	25	3.8901755E-5	45.000004	1
GACGTAA	20	7.032527E-4	45.0	35
CGTTAGA	20	7.032527E-4	45.0	13
ATATCGG	20	7.032527E-4	45.0	2
GCCGAAT	20	7.032527E-4	45.0	45
ACTACGG	20	7.032527E-4	45.0	2
ATGACGC	20	7.032527E-4	45.0	36
GCGATAT	40	6.8121153E-9	45.0	9
TATAGCG	35	1.2115561E-7	45.0	1
CGCAATA	20	7.032527E-4	45.0	25
TCGACCC	20	7.032527E-4	45.0	40
TATCGTG	35	1.2115561E-7	45.0	1
CCCGCTT	20	7.032527E-4	45.0	44
AATACGG	115	0.0	43.04348	2
CGTTTTT	2415	0.0	41.92547	1
CGACGGT	175	0.0	41.142857	28
CGGTCTA	165	0.0	40.909092	31
ACGGTCT	165	0.0	40.909092	30
>>END_MODULE