FastQCFastQC Report
Thu 26 May 2016
SRR1547045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547045_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences967202
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44120.45616117419112034No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.032733E-445.019
GCGGTAT253.890346E-545.016
ATAGCGC207.032733E-445.028
TTCGTAG207.032733E-445.01
AACGGAT207.032733E-445.029
ACGCTAG453.8380676E-1045.01
ATGCGCG453.8380676E-1045.036
CGTAAAT207.032733E-445.016
ATACGAG207.032733E-445.01
TCGACGG453.8380676E-1045.02
CGATATC207.032733E-445.031
CGTCAAG207.032733E-445.022
ATACCGG207.032733E-445.02
ACCTTCG207.032733E-445.034
CGGTCTA1550.043.5483931
CGTTTTT26750.040.87851
CGTTAGG950.040.2631572
TTCGAAT451.927765E-840.023
TAGTACG451.927765E-840.01
TCACGAC1700.039.70588325