##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547045_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	967202
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42027621944537	31.0	31.0	33.0	30.0	34.0
2	31.824847343160993	31.0	31.0	34.0	30.0	34.0
3	31.955185162975262	33.0	31.0	34.0	30.0	34.0
4	35.691592862711204	37.0	35.0	37.0	33.0	37.0
5	35.60077522585768	37.0	35.0	37.0	33.0	37.0
6	35.652343564219265	37.0	35.0	37.0	33.0	37.0
7	35.995207826286546	37.0	35.0	37.0	35.0	37.0
8	35.978766586504165	37.0	35.0	37.0	35.0	37.0
9	37.76305053132644	39.0	38.0	39.0	35.0	39.0
10	37.18125479475849	39.0	37.0	39.0	33.0	39.0
11	36.90158208936706	39.0	37.0	39.0	33.0	39.0
12	36.28169916935656	38.0	35.0	39.0	32.0	39.0
13	36.03460394002494	38.0	35.0	39.0	32.0	39.0
14	36.98691379877213	39.0	35.0	41.0	31.0	41.0
15	37.25276209106267	39.0	35.0	41.0	32.0	41.0
16	37.37317954263949	39.0	35.0	41.0	32.0	41.0
17	37.31120283043253	39.0	35.0	41.0	32.0	41.0
18	37.23016081439037	39.0	35.0	41.0	32.0	41.0
19	37.192636078089166	39.0	35.0	41.0	32.0	41.0
20	37.03240377914851	39.0	35.0	41.0	32.0	41.0
21	36.93284960122084	39.0	35.0	41.0	32.0	41.0
22	36.8388816400297	38.0	35.0	41.0	31.0	41.0
23	36.8005111651961	38.0	35.0	41.0	31.0	41.0
24	36.71292036203399	38.0	35.0	41.0	31.0	41.0
25	36.64800114143684	38.0	35.0	41.0	31.0	41.0
26	36.54835701332296	38.0	35.0	41.0	31.0	41.0
27	36.46461545778441	38.0	35.0	40.0	31.0	41.0
28	36.38634328713133	38.0	35.0	40.0	31.0	41.0
29	36.417836191405726	38.0	35.0	40.0	31.0	41.0
30	36.40771317677176	38.0	35.0	40.0	31.0	41.0
31	36.32082646644651	38.0	35.0	40.0	30.0	41.0
32	36.25418992102994	38.0	35.0	41.0	30.0	41.0
33	36.150205437954014	38.0	35.0	41.0	30.0	41.0
34	36.049535670935335	38.0	35.0	41.0	30.0	41.0
35	35.97716402571542	38.0	35.0	41.0	30.0	41.0
36	35.87442437050378	38.0	35.0	41.0	29.0	41.0
37	35.843898172253574	38.0	35.0	41.0	29.0	41.0
38	35.786051931240834	38.0	35.0	41.0	29.0	41.0
39	35.731989801509926	38.0	35.0	40.0	29.0	41.0
40	35.60372186988861	38.0	35.0	40.0	29.0	41.0
41	35.57306333113455	38.0	35.0	40.0	29.0	41.0
42	35.524765250692205	38.0	35.0	40.0	28.0	41.0
43	35.452854729415364	38.0	34.0	40.0	28.0	41.0
44	35.33779603433409	37.0	34.0	40.0	28.0	41.0
45	35.22643977162992	37.0	34.0	40.0	27.0	41.0
46	35.188759948800765	37.0	34.0	40.0	27.0	41.0
47	35.19363586923931	37.0	34.0	40.0	27.0	41.0
48	35.12913951790836	37.0	34.0	40.0	27.0	41.0
49	35.060571628263794	37.0	34.0	40.0	27.0	41.0
50	34.98326202799415	37.0	34.0	40.0	27.0	41.0
51	34.61530476570561	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	5.0
13	6.0
14	13.0
15	27.0
16	68.0
17	158.0
18	365.0
19	711.0
20	1202.0
21	1998.0
22	2968.0
23	4556.0
24	6560.0
25	9257.0
26	12368.0
27	14768.0
28	16786.0
29	18825.0
30	22439.0
31	27737.0
32	34725.0
33	45449.0
34	79044.0
35	120617.0
36	65866.0
37	85389.0
38	131108.0
39	263983.0
40	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.47883482457646	22.794204313059733	28.45072694225198	13.276233920111826
2	32.03157148144855	25.520005128194523	28.810320905043618	13.638102485313306
3	29.946174635701745	24.45693867465121	31.287466320375685	14.309420369271361
4	26.9762676255839	27.232367178727916	30.55876642107853	15.232598774609645
5	24.62453551584881	31.46323105204497	28.94193767175833	14.97029576034789
6	22.815709644934564	40.30667843945732	26.53633884131753	10.341273074290584
7	86.6244073109857	4.564299908395557	6.7893780203101315	2.0219147603086016
8	86.93313289261188	3.748441380394168	6.946015413533058	2.3724103134608905
9	82.90398489664	5.520873612751007	8.087142086141261	3.487999404467733
10	45.486982036844424	28.070351384715913	14.780056286070542	11.662610292369123
11	37.811542986883815	24.301645364670463	23.137565885926623	14.7492457625191
12	36.129164331752825	22.4956110512592	25.63063351812755	15.744591098860425
13	24.823253053653733	30.263895235948645	26.60209552916557	18.310756181232048
14	17.349323099001033	33.622449085092875	29.580687384848254	19.447540431057835
15	16.113386862310044	27.962928116360388	38.527835963945485	17.395849057384083
16	20.67386130301633	24.747364045980053	37.411833308864125	17.16694134213949
17	21.290485338119648	25.072218626512356	27.58327629595472	26.054019739413274
18	22.76670230210442	26.369775910306224	31.32799559967825	19.53552618791111
19	26.58183088951429	27.73360683704128	26.39614062005662	19.288421653387815
20	28.34392402000823	26.650068961809424	27.23815707577114	17.7678499424112
21	24.780035607866814	27.280960957483547	29.76213862254214	18.176864812107503
22	24.244883695443146	23.041929193694802	29.479364186591837	23.233822924270214
23	21.410625701766538	27.136833877514725	29.6757037309683	21.776836689750432
24	20.371339182507896	25.648726946387622	35.120481554008364	18.859452317096117
25	20.62661160750288	27.2351587362309	32.14064900610214	19.997580650164082
26	19.29379798635652	32.09215861836514	27.112743770174173	21.501299625104167
27	18.77694628423018	32.30845262933699	30.08213382519887	18.83246726123395
28	17.18865345605158	29.330894683840604	34.45298913773958	19.02746272236823
29	18.240450288564332	27.01752064201687	33.955574947115494	20.786454122303304
30	20.490032071893978	29.168570784593083	31.40285069716564	18.9385464463473
31	26.535201540112613	27.48029884140025	26.588861478781062	19.39563813970608
32	26.992913579583167	27.014005347383485	27.33151916559312	18.661561907440223
33	25.636113242114885	27.809289062677706	26.488778972748193	20.065818722459216
34	20.496338924030347	27.39686228936665	29.19255750091501	22.914241285687993
35	20.54772426028896	27.79595162127456	29.882485768226285	21.773838350210195
36	26.05691468793489	27.939665137168863	27.036441198425976	18.966978976470273
37	21.48641131842159	30.868319130853738	28.67611936286319	18.96915018786148
38	21.116064689692536	31.925285514297947	25.625670749233354	21.332979046776167
39	21.312300843050366	30.373696497732634	26.960138626677775	21.35386403253922
40	23.61130353328467	26.594858157861545	27.91154278010178	21.882295528752007
41	19.522602310582485	26.026827901513848	29.357672957665514	25.09289683023815
42	21.397495042400656	26.18129408334557	26.938736685821574	25.482474188432196
43	21.418380028163714	25.983507064708306	27.440183126172197	25.15792978095579
44	19.889537035696783	28.14386239896113	29.889102793418544	22.07749777192355
45	20.41993296126352	32.373278798017374	26.000876755837975	21.20591148488113
46	20.921689574670026	31.515857080527127	27.10798778331724	20.454465561485605
47	20.586289110237573	28.086687165659292	28.907301680517616	22.41972204358552
48	20.935854144222198	26.738261500699956	31.249004861445695	21.07687949363215
49	20.65338988132779	26.757285448127693	31.196999179075313	21.392325491469208
50	19.837531353326398	30.19503681754173	29.36542728406269	20.60200454506918
51	18.720391397040125	31.488355069571817	27.358194048399405	22.433059484988657
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	899.0
1	1614.5
2	2330.0
3	5172.0
4	8014.0
5	5725.0
6	3436.0
7	3563.5
8	3691.0
9	3729.5
10	3768.0
11	3756.0
12	3744.0
13	3850.0
14	3956.0
15	3689.0
16	3422.0
17	3459.5
18	3497.0
19	3573.5
20	3650.0
21	3961.0
22	4272.0
23	5343.5
24	6415.0
25	6792.0
26	8539.5
27	9910.0
28	12266.0
29	14622.0
30	17754.5
31	20887.0
32	24344.5
33	27802.0
34	30383.0
35	32964.0
36	34931.0
37	36898.0
38	40165.0
39	43432.0
40	52111.0
41	60790.0
42	69224.0
43	77658.0
44	79700.0
45	81742.0
46	80974.0
47	80206.0
48	78407.5
49	76609.0
50	71825.0
51	67041.0
52	62274.5
53	57508.0
54	51944.0
55	46380.0
56	43387.5
57	40395.0
58	37975.5
59	35556.0
60	32404.0
61	29252.0
62	26366.0
63	23480.0
64	19683.0
65	15886.0
66	13318.5
67	10751.0
68	9273.5
69	7796.0
70	6275.5
71	4755.0
72	3907.0
73	3059.0
74	2414.5
75	1396.5
76	1023.0
77	744.5
78	466.0
79	326.0
80	186.0
81	126.0
82	66.0
83	48.5
84	31.0
85	21.5
86	12.0
87	8.5
88	5.0
89	2.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	967202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.207330103841453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9962089256279	16.96506009741659
2	5.994378482051084	2.542495264724428
3	2.0354831917698846	1.295014919060543
4	1.1082762506948518	0.9401432117893387
5	0.6996320798938961	0.7418664234773517
6	0.5613275707926255	0.7142555454111986
7	0.4676540268431304	0.69423853251571
8	0.40986646887955597	0.6953738803219682
9	0.36491501563244244	0.6964985876729104
>10	5.933546532855311	31.471045027133254
>50	1.9364912914266257	29.24806553801477
>100	0.48927567601406785	13.181871988444998
>500	0.0024537395988669403	0.3548941518151041
>1k	4.90747919773388E-4	0.4591768322018296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4412	0.45616117419112034	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0339101862899373E-4	0.0	0.0	0.036703811613292774	0.0
2	1.0339101862899373E-4	0.0	0.0	0.12510313254108243	0.0
3	1.0339101862899373E-4	0.0	0.0	0.17938341732130414	0.0
4	1.0339101862899373E-4	0.0	0.0	0.2735726352923174	0.0
5	1.0339101862899373E-4	0.0	0.0	0.4873852618170765	0.0
6	1.0339101862899373E-4	0.0	0.0	0.6660449420079777	0.0
7	1.0339101862899373E-4	0.0	0.0	0.7809123637047897	0.0
8	1.0339101862899373E-4	0.0	0.0	1.0206761359054262	0.0
9	1.0339101862899373E-4	0.0	0.0	1.092532893852577	0.0
10	1.0339101862899373E-4	0.0	0.0	1.237797275026313	0.0
11	1.0339101862899373E-4	0.0	0.0	1.4456132224705904	0.0
12	1.0339101862899373E-4	0.0	0.0	1.6128998906123022	0.0
13	1.0339101862899373E-4	0.0	0.0	1.681137962907438	0.0
14	1.0339101862899373E-4	0.0	0.0	1.7126722235892813	0.0
15	1.0339101862899373E-4	0.0	0.0	1.76726268142539	0.0
16	1.0339101862899373E-4	0.0	0.0	1.8698265719053517	0.0
17	1.0339101862899373E-4	0.0	0.0	1.9908974547199034	0.0
18	1.0339101862899373E-4	0.0	0.0	2.1411246047878314	0.0
19	1.0339101862899373E-4	0.0	0.0	2.233866348498039	0.0
20	1.0339101862899373E-4	0.0	0.0	2.340048924630015	0.0
21	1.0339101862899373E-4	0.0	0.0	2.491930330996007	0.0
22	1.0339101862899373E-4	0.0	0.0	2.637401494207001	0.0
23	1.0339101862899373E-4	0.0	0.0	2.7970372269701675	0.0
24	1.0339101862899373E-4	0.0	0.0	2.920486103213186	0.0
25	1.0339101862899373E-4	0.0	0.0	3.0332857045374184	0.0
26	1.0339101862899373E-4	0.0	0.0	3.153426068184309	0.0
27	1.0339101862899373E-4	0.0	0.0	3.2728426947007967	0.0
28	1.0339101862899373E-4	0.0	0.0	3.400530602707604	0.0
29	1.0339101862899373E-4	0.0	0.0	3.5405220419312613	0.0
30	1.0339101862899373E-4	0.0	0.0	3.69788317228459	0.0
31	1.0339101862899373E-4	0.0	0.0	3.847386585222115	0.0
32	1.0339101862899373E-4	0.0	0.0	3.9893424537997233	0.0
33	1.0339101862899373E-4	0.0	0.0	4.124784688203705	0.0
34	1.0339101862899373E-4	0.0	0.0	4.277803395774616	0.0
35	1.0339101862899373E-4	0.0	0.0	4.461012280785193	0.0
36	1.0339101862899373E-4	0.0	0.0	4.6137208153002165	0.0
37	1.0339101862899373E-4	0.0	0.0	4.7799735732556385	0.0
38	1.0339101862899373E-4	0.0	0.0	4.971246957719277	0.0
39	1.0339101862899373E-4	0.0	0.0	5.2538146116323166	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.032733E-4	45.0	19
GCGGTAT	25	3.890346E-5	45.0	16
ATAGCGC	20	7.032733E-4	45.0	28
TTCGTAG	20	7.032733E-4	45.0	1
AACGGAT	20	7.032733E-4	45.0	29
ACGCTAG	45	3.8380676E-10	45.0	1
ATGCGCG	45	3.8380676E-10	45.0	36
CGTAAAT	20	7.032733E-4	45.0	16
ATACGAG	20	7.032733E-4	45.0	1
TCGACGG	45	3.8380676E-10	45.0	2
CGATATC	20	7.032733E-4	45.0	31
CGTCAAG	20	7.032733E-4	45.0	22
ATACCGG	20	7.032733E-4	45.0	2
ACCTTCG	20	7.032733E-4	45.0	34
CGGTCTA	155	0.0	43.54839	31
CGTTTTT	2675	0.0	40.8785	1
CGTTAGG	95	0.0	40.263157	2
TTCGAAT	45	1.927765E-8	40.0	23
TAGTACG	45	1.927765E-8	40.0	1
TCACGAC	170	0.0	39.705883	25
>>END_MODULE