FastQCFastQC Report
Thu 26 May 2016
SRR1547044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547044_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1143440
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44200.38655285804239836No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27570.24111453158888965No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25340.22161197789127546No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22790.19931085146575248No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA21400.18715455117889876No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16830.1471874344084517No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA14520.1269852375288603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11440.10004897502273839No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCA302.1654923E-645.00000426
GGTGTAT600.045.00000438
TTTCGAT302.1654923E-645.00000437
GACAATC302.1654923E-645.00000433
TAGCGAT302.1654923E-645.00000438
TAACGTT302.1654923E-645.00000428
CCGTATT600.045.00000426
TAACCGT302.1654923E-645.00000442
CCATCGG302.1654923E-645.00000442
GTTCGCG302.1654923E-645.0000041
CTACGTC302.1654923E-645.00000430
AGCGATC302.1654923E-645.00000439
GCGATAT302.1654923E-645.0000049
CATCGGT302.1654923E-645.00000443
ACGTATG302.1654923E-645.0000041
CGGTCTA1250.045.00000431
ATCGGTC302.1654923E-645.00000444
CGACTTG302.1654923E-645.00000419
CGCAATA302.1654923E-645.00000425
ACTCAAT302.1654923E-645.00000410