##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547044_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1143440
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46982264045337	31.0	31.0	33.0	30.0	34.0
2	31.865051948506263	31.0	31.0	34.0	30.0	34.0
3	31.972508395718183	33.0	31.0	34.0	30.0	34.0
4	35.68701986986637	37.0	35.0	37.0	33.0	37.0
5	35.581159483663335	37.0	35.0	37.0	33.0	37.0
6	35.66153799062478	37.0	35.0	37.0	33.0	37.0
7	36.02565154271321	37.0	35.0	37.0	35.0	37.0
8	36.030459840481356	37.0	35.0	37.0	35.0	37.0
9	37.8225582452949	39.0	38.0	39.0	35.0	39.0
10	37.268116385643324	39.0	37.0	39.0	34.0	39.0
11	36.863296718673475	39.0	35.0	39.0	33.0	39.0
12	35.65678916252711	37.0	35.0	39.0	32.0	39.0
13	35.09319946827118	37.0	35.0	39.0	30.0	39.0
14	35.96995644721192	37.0	35.0	41.0	30.0	41.0
15	36.41878629399007	38.0	35.0	41.0	31.0	41.0
16	36.66065119289163	37.0	35.0	41.0	32.0	41.0
17	36.63751224375569	37.0	35.0	41.0	32.0	41.0
18	36.59849139438886	37.0	35.0	40.0	32.0	41.0
19	36.54915255719583	37.0	35.0	40.0	32.0	41.0
20	36.336177674386064	36.0	35.0	40.0	32.0	41.0
21	36.160441299937034	36.0	35.0	40.0	31.0	41.0
22	36.030637374938784	36.0	35.0	40.0	31.0	41.0
23	36.08912492129014	36.0	35.0	40.0	31.0	41.0
24	36.00650143426852	35.0	35.0	40.0	31.0	41.0
25	35.95820593997062	35.0	35.0	40.0	31.0	41.0
26	35.81759515147275	35.0	35.0	40.0	31.0	41.0
27	35.74791768697964	35.0	35.0	40.0	31.0	41.0
28	35.78478101168404	36.0	35.0	40.0	31.0	41.0
29	35.884471419576016	36.0	35.0	40.0	31.0	41.0
30	35.89721192191982	36.0	35.0	40.0	31.0	41.0
31	35.72970859861471	36.0	35.0	40.0	31.0	41.0
32	35.543312285734274	35.0	34.0	40.0	30.0	41.0
33	35.45963583572378	35.0	34.0	40.0	30.0	41.0
34	35.41122577485483	35.0	34.0	40.0	30.0	41.0
35	35.34266074302106	35.0	34.0	40.0	30.0	41.0
36	35.192617015322185	35.0	34.0	40.0	29.0	41.0
37	35.12627509969915	35.0	34.0	40.0	29.0	41.0
38	35.14505177359547	35.0	34.0	40.0	29.0	41.0
39	35.1483812005877	36.0	34.0	40.0	29.0	41.0
40	34.98691579794305	35.0	34.0	40.0	29.0	41.0
41	35.03578062688029	35.0	34.0	40.0	29.0	41.0
42	34.967248828097674	35.0	34.0	40.0	29.0	41.0
43	34.861598334849226	35.0	34.0	40.0	28.0	41.0
44	34.72342930105646	35.0	34.0	40.0	27.0	41.0
45	34.65011369201707	35.0	34.0	40.0	27.0	41.0
46	34.64768330651368	35.0	34.0	40.0	27.0	41.0
47	34.62948996012034	35.0	34.0	40.0	27.0	41.0
48	34.603341670747916	35.0	34.0	40.0	27.0	41.0
49	34.60176659903449	35.0	34.0	40.0	27.0	41.0
50	34.459195060519136	35.0	34.0	40.0	27.0	41.0
51	34.07016371650458	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	0.0
13	4.0
14	14.0
15	25.0
16	70.0
17	179.0
18	404.0
19	837.0
20	1494.0
21	2428.0
22	3770.0
23	5566.0
24	8242.0
25	11857.0
26	15927.0
27	19370.0
28	21457.0
29	24308.0
30	29049.0
31	35524.0
32	45326.0
33	60956.0
34	119504.0
35	228699.0
36	61484.0
37	76718.0
38	118529.0
39	251340.0
40	355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.42825159168824	22.943923598964528	30.447071993283426	13.180752816063807
2	30.246711677044708	26.697071993283426	29.92846148464283	13.127754845029035
3	30.37911914923389	24.86007136360456	31.87390680752816	12.886902679633385
4	28.094784160078362	27.65453368781921	30.182869236689285	14.067812915413139
5	24.140925627929754	32.8557685580354	28.80439725739873	14.198908556636116
6	22.744437836703284	41.944658224305606	25.325508990414892	9.985394948576227
7	86.95733925697894	5.106345763660533	6.260232281536417	1.67608269782411
8	88.48964528090674	3.8777723361085847	5.667547051003988	1.96503533198069
9	84.64764220247673	6.06809277268593	7.0299272371090735	2.2543377877282587
10	57.5059469670468	24.194710697544252	10.677079689358427	7.622262646050514
11	54.33306513678025	17.60459665570559	16.928041698733644	11.134296508780523
12	47.61535366962849	21.767123766878893	19.467921360106345	11.149601203386272
13	21.28734345483803	45.30075911285245	20.87026866298188	12.541628769327643
14	13.183901210382704	48.03409011404184	25.94084516896383	12.841163506611627
15	12.394616245714687	24.970352620163716	51.2238508360736	11.411180298047995
16	14.464073322605472	18.84593857132862	50.40264465122788	16.287343454838034
17	14.511998880570909	20.147449800601695	27.761666550059473	37.57888476876793
18	21.77700622682432	25.086318477576437	34.91027076191143	18.22640453368782
19	31.515514587560347	24.869429091163507	25.126810326733366	18.488245994542783
20	34.29554677114672	23.814017351150913	24.3941964598055	17.496239417896874
21	22.35849716644511	30.315451619673965	26.75960260267264	20.566448611208283
22	21.279559924438537	25.01101938011614	25.590498845588748	28.11892184985657
23	18.766441614776465	31.30807038410411	25.154883509410205	24.77060449170923
24	19.003183376478	23.860018890365915	41.07762541104037	16.05917232211572
25	15.218900860561114	26.810501644161477	37.993160987896175	19.977436507381235
26	14.951374798852585	39.90703491219478	26.477034212551597	18.664556076401034
27	16.29818792415868	38.62546351360806	29.911407682082135	15.164940880151123
28	13.024644931085147	30.906650108444694	41.593349891555306	14.475355068914853
29	13.79538935143077	26.185108094871616	39.62551598684671	20.393986566850906
30	17.942174491009585	33.603162387182536	31.71106485692297	16.74359826488491
31	32.73822850346323	27.39540334429441	23.890803190372907	15.975564961869445
32	31.868572028265586	28.794864619044287	25.563212761491638	13.773350591198488
33	28.766179248583224	30.42022318617505	22.84116350661163	17.9724340586301
34	18.901822570489053	30.071013782970685	27.005702091933117	24.02146155460715
35	19.883159588609807	25.91320926327573	31.681942209473167	22.521688938641294
36	31.550059469670465	25.568022808367736	26.737826208633596	16.144091513328203
37	19.358164835933675	35.60982648849087	28.98254390260967	16.049464772965788
38	18.826436017631007	36.62684530889246	21.908101868047297	22.638616805429233
39	20.886622822360597	33.463758483173585	27.09753025956762	18.552088434898202
40	25.09690058070384	27.59270272161198	25.052473238648286	22.25792345903589
41	16.66410130833275	25.013992863639544	27.69143986566851	30.6304659623592
42	21.82292031064157	24.806985937172044	23.972661442664243	29.39743230952214
43	22.79542433358987	24.284439935632825	25.70795144476317	27.212184286014136
44	17.44297908066886	32.08030154621143	30.0230007696075	20.453718603512208
45	16.13490869656475	42.10041628769328	22.26107185335479	19.503603162387183
46	22.460120338627302	35.68661232771287	23.933831246064507	17.919436087595326
47	21.882389981109636	29.09990904638634	24.97752396277898	24.04017700972504
48	21.886675295599247	25.036643811656056	32.122455047925556	20.954225844819142
49	20.223186175050724	25.111243265934373	33.2545651717624	21.4110053872525
50	17.867312670538027	36.02235359966417	27.276289092562795	18.83404463723501
51	17.888739242986077	36.96897082487931	23.682746799132442	21.459543133002168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1491.0
1	1861.0
2	2231.0
3	4993.0
4	7755.0
5	5860.5
6	3966.0
7	4145.5
8	4325.0
9	4628.0
10	4931.0
11	4832.5
12	4734.0
13	4562.5
14	4391.0
15	4039.5
16	3688.0
17	3746.0
18	3804.0
19	3383.0
20	2962.0
21	3836.0
22	4710.0
23	4672.5
24	4635.0
25	6479.0
26	10264.5
27	12206.0
28	16176.0
29	20146.0
30	19847.0
31	19548.0
32	23851.0
33	28154.0
34	27666.5
35	27179.0
36	31057.0
37	34935.0
38	39568.0
39	44201.0
40	66936.0
41	89671.0
42	112672.5
43	135674.0
44	138800.0
45	141926.0
46	131503.0
47	121080.0
48	105792.0
49	90504.0
50	83699.0
51	76894.0
52	66283.0
53	55672.0
54	51086.0
55	46500.0
56	40545.5
57	34591.0
58	32311.5
59	30032.0
60	27555.0
61	25078.0
62	21682.5
63	18287.0
64	15026.5
65	11766.0
66	9072.0
67	6378.0
68	5215.0
69	4052.0
70	3401.5
71	2751.0
72	2550.5
73	2350.0
74	1720.5
75	767.5
76	444.0
77	318.0
78	192.0
79	136.5
80	81.0
81	75.0
82	69.0
83	51.5
84	34.0
85	19.0
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1143440.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.630922561982292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.5339298673733	13.559844737983804
2	8.148776507157875	2.7104334213088626
3	2.482916471593768	1.2387957470083877
4	1.1050241527350513	0.7351028445302692
5	0.6551809269856044	0.544813163039268
6	0.41058313558531956	0.40970257999051957
7	0.30379929640118264	0.3536723800982942
8	0.21814897156221458	0.29024149224218143
9	0.17913630464618985	0.2681281809549406
>10	2.0346901136204143	8.43114018376267
>50	1.128770341046449	13.884600090728295
>100	1.7783956733466313	54.28420065404288
>500	0.01641270195705435	1.6683920305864717
>1k	0.004235535988917251	1.6209324937231557
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4420	0.38655285804239836	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2757	0.24111453158888965	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2534	0.22161197789127546	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2279	0.19931085146575248	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2140	0.18715455117889876	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1683	0.1471874344084517	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1452	0.1269852375288603	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1144	0.10004897502273839	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05938221507031414	0.0
2	0.0	0.0	0.0	0.2011474148184426	0.0
3	0.0	0.0	0.0	0.2795949065976352	0.0
4	0.0	0.0	0.0	0.45817882879731336	0.0
5	0.0	0.0	0.0	0.7686454907996921	0.0
6	0.0	0.0	0.0	1.0564612047855593	0.0
7	0.0	0.0	0.0	1.2195655215839922	0.0
8	0.0	0.0	0.0	1.5672007276289093	0.0
9	0.0	0.0	0.0	1.6602532708318758	0.0
10	0.0	0.0	0.0	1.8305289302455747	0.0
11	0.0	0.0	0.0	2.0834499405303295	0.0
12	0.0	0.0	0.0	2.3057615616035823	0.0
13	0.0	0.0	0.0	2.386045616735465	0.0
14	0.0	0.0	0.0	2.41962848947037	0.0
15	0.0	0.0	0.0	2.474812845448821	0.0
16	0.0	0.0	0.0	2.6042468341146017	0.0
17	0.0	0.0	0.0	2.7528335548870078	0.0
18	0.0	0.0	0.0	2.9712971384593856	0.0
19	0.0	0.0	0.0	3.075369061778493	0.0
20	0.0	0.0	0.0	3.182851745609739	0.0
21	0.0	0.0	0.0	3.33712306723571	0.0
22	0.0	0.0	0.0	3.4918316658504165	0.0
23	0.0	0.0	0.0	3.6593962079339537	0.0
24	0.0	0.0	0.0	3.788305464213251	0.0
25	0.0	0.0	0.0	3.899286363954383	0.0
26	0.0	0.0	0.0	4.007206324774365	0.0
27	0.0	0.0	0.0	4.099821590988596	0.0
28	0.0	0.0	0.0	4.218586021129224	0.0
29	0.0	0.0	0.0	4.330179108654586	0.0
30	0.0	0.0	0.0	4.486461904428741	0.0
31	0.0	0.0	0.0	4.643706709578115	0.0
32	0.0	0.0	0.0	4.7730532428461485	0.0
33	0.0	0.0	0.0	4.908871475547471	0.0
34	0.0	0.0	0.0	5.041803680123137	0.0
35	0.0	0.0	0.0	5.238753235849717	0.0
36	0.0	0.0	0.0	5.404656125376058	0.0
37	0.0	0.0	0.0	5.55245574756874	0.0
38	0.0	0.0	0.0	5.694570768907857	0.0
39	0.0	0.0	0.0	5.840708738543343	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGCA	30	2.1654923E-6	45.000004	26
GGTGTAT	60	0.0	45.000004	38
TTTCGAT	30	2.1654923E-6	45.000004	37
GACAATC	30	2.1654923E-6	45.000004	33
TAGCGAT	30	2.1654923E-6	45.000004	38
TAACGTT	30	2.1654923E-6	45.000004	28
CCGTATT	60	0.0	45.000004	26
TAACCGT	30	2.1654923E-6	45.000004	42
CCATCGG	30	2.1654923E-6	45.000004	42
GTTCGCG	30	2.1654923E-6	45.000004	1
CTACGTC	30	2.1654923E-6	45.000004	30
AGCGATC	30	2.1654923E-6	45.000004	39
GCGATAT	30	2.1654923E-6	45.000004	9
CATCGGT	30	2.1654923E-6	45.000004	43
ACGTATG	30	2.1654923E-6	45.000004	1
CGGTCTA	125	0.0	45.000004	31
ATCGGTC	30	2.1654923E-6	45.000004	44
CGACTTG	30	2.1654923E-6	45.000004	19
CGCAATA	30	2.1654923E-6	45.000004	25
ACTCAAT	30	2.1654923E-6	45.000004	10
>>END_MODULE