FastQCFastQC Report
Thu 26 May 2016
SRR1547042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547042_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1299727
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60530.46571318438410525No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14320.11017698332034342No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG253.8910875E-545.0000041
AGCCGAA253.8910875E-545.00000442
GCCTTCG253.8910875E-545.0000049
ACACGGC302.1657215E-645.00000427
ACGCCGT253.8910875E-545.00000443
GTCGATA302.1657215E-645.00000416
CGTATCC351.2120836E-745.00000412
CGAGTAC253.8910875E-545.00000420
TTTCGCG502.1827873E-1145.0000041
ATCAGTC302.1657215E-645.00000413
CGTGAAT253.8910875E-545.00000435
TTATCGG351.2120836E-745.0000041
TCGATAC302.1657215E-645.00000417
GACCCGT302.1657215E-645.00000422
CGTTAGA302.1657215E-645.00000420
ACGATTT351.2120836E-745.00000417
AGACGAT302.1657215E-645.00000439
CTACGTA253.8910875E-545.00000442
TATTCGA253.8910875E-545.00000436
TATTACG700.045.0000041