##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547042_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1299727
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44377473115508	31.0	31.0	33.0	30.0	34.0
2	31.848094253639417	31.0	31.0	34.0	30.0	34.0
3	31.956514714243838	33.0	31.0	34.0	30.0	34.0
4	35.69429426333376	37.0	35.0	37.0	33.0	37.0
5	35.587768816066756	37.0	35.0	37.0	33.0	37.0
6	35.65066740938674	37.0	35.0	37.0	33.0	37.0
7	36.030251737480256	37.0	35.0	37.0	35.0	37.0
8	36.03377632379723	37.0	35.0	37.0	35.0	37.0
9	37.835791670096874	39.0	38.0	39.0	35.0	39.0
10	37.22727157318422	39.0	37.0	39.0	34.0	39.0
11	36.86422302529685	39.0	35.0	39.0	33.0	39.0
12	35.87428052198654	37.0	35.0	39.0	32.0	39.0
13	35.432975540248066	37.0	35.0	39.0	30.0	39.0
14	36.26315834017451	38.0	35.0	41.0	30.0	41.0
15	36.64494466914975	38.0	35.0	41.0	31.0	41.0
16	36.84995310553678	38.0	35.0	41.0	32.0	41.0
17	36.802447744795636	38.0	35.0	41.0	32.0	41.0
18	36.73338554942692	38.0	35.0	40.0	32.0	41.0
19	36.688259919198416	38.0	35.0	40.0	32.0	41.0
20	36.496288836040186	37.0	35.0	40.0	31.0	41.0
21	36.36620305648802	37.0	35.0	40.0	31.0	41.0
22	36.29228291787429	37.0	35.0	40.0	31.0	41.0
23	36.31877617376572	37.0	35.0	40.0	31.0	41.0
24	36.23301508701443	37.0	35.0	40.0	31.0	41.0
25	36.18747167674442	37.0	35.0	40.0	31.0	41.0
26	36.041849557637875	37.0	35.0	40.0	31.0	41.0
27	35.95512903863658	36.0	35.0	40.0	31.0	41.0
28	35.93737915731534	36.0	35.0	40.0	31.0	41.0
29	36.002758271544714	36.0	35.0	40.0	31.0	41.0
30	35.979080991623626	36.0	35.0	40.0	30.0	41.0
31	35.817957925010404	36.0	35.0	40.0	30.0	41.0
32	35.67340218368934	36.0	34.0	40.0	30.0	41.0
33	35.55386554253316	36.0	34.0	40.0	30.0	41.0
34	35.478972891999625	36.0	34.0	40.0	29.0	41.0
35	35.403596293683215	36.0	34.0	40.0	29.0	41.0
36	35.32165216233871	36.0	34.0	40.0	29.0	41.0
37	35.27728823052841	36.0	34.0	40.0	29.0	41.0
38	35.25313315796317	36.0	34.0	40.0	29.0	41.0
39	35.21301088613224	36.0	34.0	40.0	29.0	41.0
40	35.06957922702229	36.0	34.0	40.0	28.0	41.0
41	35.07847109431442	36.0	34.0	40.0	28.0	41.0
42	35.058060654275856	36.0	34.0	40.0	28.0	41.0
43	34.944100568811756	36.0	34.0	40.0	27.0	41.0
44	34.82555105802988	35.0	34.0	40.0	27.0	41.0
45	34.709518998989786	35.0	34.0	40.0	27.0	41.0
46	34.72625482120476	35.0	34.0	40.0	27.0	41.0
47	34.714092267068395	35.0	34.0	40.0	27.0	41.0
48	34.66375477311774	35.0	34.0	40.0	27.0	41.0
49	34.63008077850195	36.0	34.0	40.0	27.0	41.0
50	34.51514356476399	35.0	34.0	40.0	27.0	41.0
51	34.15407851033332	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	9.0
13	13.0
14	15.0
15	42.0
16	89.0
17	232.0
18	527.0
19	1000.0
20	1723.0
21	2754.0
22	4233.0
23	6368.0
24	9402.0
25	13557.0
26	18185.0
27	22006.0
28	24407.0
29	27733.0
30	33216.0
31	40340.0
32	51017.0
33	68145.0
34	129226.0
35	216280.0
36	80340.0
37	99530.0
38	151596.0
39	297479.0
40	259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.64152625897592	23.56679518083413	29.869426425703242	12.922252134486703
2	31.70019550259401	24.39743115284979	30.504482864478465	13.39789048007774
3	28.723185715154028	24.30648897807001	33.11079942172472	13.859525885051246
4	26.82270969211227	26.868026901033833	31.427984492127965	14.881278914725938
5	24.095829354933766	30.899411953433297	30.723605803372557	14.281152888260381
6	22.163115792778022	39.27863312834157	27.33589438397448	11.22235669490593
7	87.49091155296458	4.17680020496612	6.643010416802913	1.6892778252663827
8	89.20157848532807	2.5162976532764185	6.31132537832945	1.9707984830660594
9	85.47925833655837	4.598350268941093	7.572513304717067	2.34987808978347
10	54.12859777476346	23.118624141839017	12.665967545492245	10.086810537905269
11	47.40487810132436	20.038900476792435	19.498171539100134	13.058049882783077
12	42.69342715816475	20.20047286853316	22.93720142768443	14.168898545617656
13	24.64656039306716	36.350402815360454	23.61288178209732	15.390155009475068
14	15.645131631488768	38.35674722460948	30.042924398739117	15.95519674516264
15	15.263359151575678	24.55284840585754	46.15738535861762	14.026407083949168
16	19.307208359909428	20.360660354058968	44.03347779956868	16.29865348646293
17	19.325904593810854	20.09268100147185	28.306098126760464	32.27531627795683
18	24.306412038835848	23.214336549136856	33.46664337972513	19.01260803230217
19	30.314673773800187	25.83157847763415	25.157975482543645	18.69577226602202
20	32.82081544816719	25.448728848442787	24.897920871075232	16.832534832314785
21	25.753023519554493	26.90834305973485	28.041119404305675	19.297514016404985
22	22.2813713956854	22.412014215292903	29.353087225240376	25.953527163781313
23	20.62386947412803	27.434222725233838	28.728263704608736	23.213644096029398
24	21.56360528018576	22.758394647491357	37.5966645303206	18.081335542002282
25	19.69252004459398	24.128682407921048	34.82569801196713	21.353099535517845
26	16.392673230609198	34.85747391567614	27.837999826117333	20.911853027597335
27	16.8363817940229	33.53750441438856	31.157850840984295	18.46826295060424
28	15.294750359113877	28.623318589211426	38.55825107888041	17.523679972794287
29	17.790274419166487	25.982379376592164	36.72071134938337	19.50663485485798
30	20.87715343299016	28.9600816171396	32.76241856943805	17.4003463804322
31	31.36397104930497	25.17913377193826	26.21435116759135	17.24254401116542
32	31.915779236716634	25.759255597521637	26.91819128170762	15.406773884054111
33	29.106573918984523	26.453016671962647	26.026619436235453	18.41378997281737
34	20.41713375193406	27.2647255923744	29.383785979671117	22.934354676020426
35	19.490939251088882	27.24564466230216	31.705042674346227	21.55837341226273
36	29.925207370470876	25.650078824245398	26.633362236838963	17.791351568444757
37	21.088582448468024	33.38162552597584	27.443763190269955	18.08602883528618
38	20.757743741570344	33.92012322587743	23.67312520244636	21.64900783010586
39	21.62777260147708	29.858577993686364	27.832383262023484	20.68126614281307
40	25.464424452211887	24.488142509927087	26.987359653219485	23.060073384641544
41	17.59307916200864	24.844063407161656	28.193689905649418	29.36916752518029
42	22.961360347211375	23.79138080535374	25.801956872481686	27.4453019749532
43	21.919833934356983	24.624171075925943	26.72922852260513	26.72676646711194
44	20.07059944126728	28.676714417720028	29.22613748887266	22.026548652140026
45	19.15240662077498	34.84624078748845	24.045511095791657	21.955841495944917
46	22.71030762613995	31.2768758362333	26.46009508150558	19.55272145612117
47	20.82014146047593	27.733054710720022	27.246798750814595	24.200005077989456
48	21.89298214163436	24.704957271796307	32.49574718383168	20.906313402737652
49	21.714252300675447	23.973419033381628	32.254234927796375	22.05809373814655
50	19.58665165838672	31.286647118971906	28.392885582895484	20.733815639745885
51	18.901430838937717	32.36110352404774	26.21527443840129	22.522191198613246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1475.0
1	2191.0
2	2907.0
3	7035.0
4	11163.0
5	7952.5
6	4742.0
7	4841.5
8	4941.0
9	5067.0
10	5193.0
11	5197.0
12	5201.0
13	4976.5
14	4752.0
15	4506.5
16	4261.0
17	3977.5
18	3694.0
19	3935.5
20	4177.0
21	4292.5
22	4408.0
23	4771.5
24	5135.0
25	6080.5
26	8192.0
27	9358.0
28	11094.0
29	12830.0
30	16753.0
31	20676.0
32	22754.5
33	24833.0
34	27817.5
35	30802.0
36	33407.5
37	36013.0
38	43533.5
39	51054.0
40	70773.0
41	90492.0
42	110882.5
43	131273.0
44	130625.0
45	129977.0
46	124337.0
47	118697.0
48	114683.0
49	110669.0
50	102079.5
51	93490.0
52	83731.0
53	73972.0
54	70850.0
55	67728.0
56	61048.5
57	54369.0
58	51727.0
59	49085.0
60	45185.5
61	41286.0
62	35914.5
63	30543.0
64	26269.5
65	21996.0
66	16986.5
67	11977.0
68	10112.5
69	8248.0
70	6510.5
71	4773.0
72	3837.5
73	2902.0
74	2255.5
75	1212.0
76	815.0
77	732.5
78	650.0
79	482.5
80	315.0
81	222.0
82	129.0
83	93.0
84	57.0
85	37.5
86	18.0
87	13.0
88	8.0
89	8.0
90	8.0
91	4.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1299727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.767572329501206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.77487638895697	15.15948335036269
2	7.84072019320749	2.943025666828045
3	2.450428847885272	1.379658019229494
4	1.1881994770692774	0.8919847851109269
5	0.6939099559071739	0.6511502643824438
6	0.4814546623747147	0.5421441119695825
7	0.35783330011630177	0.4700963639285763
8	0.2846750569215028	0.4274127776943341
9	0.22231415321840994	0.37550672553584896
>10	2.9811251146665305	13.76561114450264
>50	1.325570222369596	18.079495793187235
>100	1.391478002073743	43.80274911209709
>500	0.005355007112776393	0.6690239866150516
>1k	0.0016476944962388902	0.37471218663313555
>5k	4.1192362405972254E-4	0.46794571192291506
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6053	0.46571318438410525	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1432	0.11017698332034342	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.6939234162251E-5	0.0	0.0	0.05708891174839024	0.0
2	7.6939234162251E-5	0.0	0.0	0.19196338923481623	0.0
3	7.6939234162251E-5	0.0	0.0	0.27728899992075257	0.0
4	7.6939234162251E-5	0.0	0.0	0.43455279454839363	0.0
5	7.6939234162251E-5	0.0	0.0	0.7386935871917718	0.0
6	7.6939234162251E-5	0.0	0.0	0.9998253479384517	0.0
7	7.6939234162251E-5	0.0	0.0	1.16793757458297	0.0
8	7.6939234162251E-5	0.0	0.0	1.5154720952938578	0.0
9	7.6939234162251E-5	0.0	0.0	1.6164933097488934	0.0
10	7.6939234162251E-5	0.0	0.0	1.8123805999259845	0.0
11	7.6939234162251E-5	0.0	0.0	2.128523913098674	0.0
12	7.6939234162251E-5	0.0	0.0	2.3611112179711586	0.0
13	7.6939234162251E-5	0.0	0.0	2.461439979318734	0.0
14	7.6939234162251E-5	0.0	0.0	2.5029871657663496	0.0
15	7.6939234162251E-5	0.0	0.0	2.5642307961595012	0.0
16	7.6939234162251E-5	0.0	0.0	2.710107584131129	0.0
17	7.6939234162251E-5	0.0	0.0	2.8757577552824554	0.0
18	7.6939234162251E-5	0.0	0.0	3.0922647602150297	0.0
19	7.6939234162251E-5	0.0	0.0	3.2129824186156015	0.0
20	7.6939234162251E-5	0.0	0.0	3.3438560559255905	0.0
21	7.6939234162251E-5	0.0	0.0	3.5284332786808306	0.0
22	7.6939234162251E-5	0.0	0.0	3.722243209535541	0.0
23	7.6939234162251E-5	0.0	0.0	3.9146682341753305	0.0
24	7.6939234162251E-5	0.0	0.0	4.05477457958479	0.0
25	7.6939234162251E-5	0.0	0.0	4.1792622604593115	0.0
26	7.6939234162251E-5	0.0	0.0	4.305596482953728	0.0
27	7.6939234162251E-5	0.0	0.0	4.440086264269342	0.0
28	7.6939234162251E-5	0.0	0.0	4.581962212064534	0.0
29	7.6939234162251E-5	0.0	0.0	4.748535654025807	0.0
30	7.6939234162251E-5	0.0	0.0	4.9426533418171665	0.0
31	7.6939234162251E-5	0.0	0.0	5.1250762660158635	0.0
32	7.6939234162251E-5	0.0	0.0	5.28834132090816	0.0
33	7.6939234162251E-5	0.0	0.0	5.45960805615333	0.0
34	7.6939234162251E-5	0.0	0.0	5.636106659321534	0.0
35	7.6939234162251E-5	0.0	0.0	5.863692913973473	0.0
36	7.6939234162251E-5	0.0	0.0	6.053117308480934	0.0
37	7.6939234162251E-5	0.0	0.0	6.232770420249791	0.0
38	7.6939234162251E-5	0.0	0.0	6.471743681557743	0.0
39	1.53878468324502E-4	0.0	0.0	6.855978216964024	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	25	3.8910875E-5	45.000004	1
AGCCGAA	25	3.8910875E-5	45.000004	42
GCCTTCG	25	3.8910875E-5	45.000004	9
ACACGGC	30	2.1657215E-6	45.000004	27
ACGCCGT	25	3.8910875E-5	45.000004	43
GTCGATA	30	2.1657215E-6	45.000004	16
CGTATCC	35	1.2120836E-7	45.000004	12
CGAGTAC	25	3.8910875E-5	45.000004	20
TTTCGCG	50	2.1827873E-11	45.000004	1
ATCAGTC	30	2.1657215E-6	45.000004	13
CGTGAAT	25	3.8910875E-5	45.000004	35
TTATCGG	35	1.2120836E-7	45.000004	1
TCGATAC	30	2.1657215E-6	45.000004	17
GACCCGT	30	2.1657215E-6	45.000004	22
CGTTAGA	30	2.1657215E-6	45.000004	20
ACGATTT	35	1.2120836E-7	45.000004	17
AGACGAT	30	2.1657215E-6	45.000004	39
CTACGTA	25	3.8910875E-5	45.000004	42
TATTCGA	25	3.8910875E-5	45.000004	36
TATTACG	70	0.0	45.000004	1
>>END_MODULE