FastQCFastQC Report
Thu 26 May 2016
SRR1547041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547041_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1495123
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50420.3372297797572507No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA253.8913702E-545.00000411
TCGATCA502.1827873E-1145.00000417
CTAACCG253.8913702E-545.00000418
TTAAGCG253.8913702E-545.0000041
TACAGCG650.045.0000041
CGAACTA406.8175723E-945.033
CCGATTA207.033968E-445.010
ACCGCTA207.033968E-445.017
CTACGCG351.2122655E-745.01
CATACGT207.033968E-445.011
CGCAATA207.033968E-445.014
TATCGCG750.045.01
TACGTTC207.033968E-445.031
ATCGATA207.033968E-445.036
GTTTACG551.8189894E-1244.9999961
CGGTCTA3800.044.40789431
CGACGGT3900.043.84615328
GCGATAT1350.043.3333369
AATAGCG1350.043.3333361
TCACGAC4150.042.83132625