##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547041_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1495123
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.434328814418613	31.0	31.0	33.0	30.0	34.0
2	31.85155669466659	31.0	31.0	34.0	30.0	34.0
3	31.990408147022016	33.0	31.0	34.0	30.0	34.0
4	35.714262304840474	37.0	35.0	37.0	33.0	37.0
5	35.613483305386914	37.0	35.0	37.0	33.0	37.0
6	35.68179674849494	37.0	35.0	37.0	33.0	37.0
7	36.028933405479016	37.0	35.0	37.0	35.0	37.0
8	36.02705396144665	37.0	35.0	37.0	35.0	37.0
9	37.84554648681078	39.0	38.0	39.0	35.0	39.0
10	37.20290638295311	39.0	37.0	39.0	33.0	39.0
11	36.92854634702295	39.0	37.0	39.0	33.0	39.0
12	36.27394602317	38.0	35.0	39.0	32.0	39.0
13	35.998606134746105	38.0	35.0	39.0	32.0	39.0
14	36.91821007368625	39.0	35.0	41.0	31.0	41.0
15	37.200762746610145	39.0	35.0	41.0	32.0	41.0
16	37.31883463768533	39.0	35.0	41.0	32.0	41.0
17	37.28435051831856	39.0	35.0	41.0	32.0	41.0
18	37.20773675476867	39.0	35.0	41.0	32.0	41.0
19	37.18859384813156	39.0	35.0	41.0	32.0	41.0
20	37.05029552752516	39.0	35.0	41.0	32.0	41.0
21	36.93163037422339	39.0	35.0	41.0	32.0	41.0
22	36.86557427047808	38.0	35.0	41.0	32.0	41.0
23	36.83833437115207	38.0	35.0	41.0	32.0	41.0
24	36.77841622394947	38.0	35.0	41.0	31.0	41.0
25	36.71885724452102	38.0	35.0	41.0	31.0	41.0
26	36.60255042561716	38.0	35.0	41.0	31.0	41.0
27	36.512729053061186	38.0	35.0	40.0	31.0	41.0
28	36.49632973340655	38.0	35.0	40.0	31.0	41.0
29	36.54059431899583	38.0	35.0	40.0	31.0	41.0
30	36.53036572910724	38.0	35.0	40.0	31.0	41.0
31	36.414543151299256	38.0	35.0	40.0	31.0	41.0
32	36.35223857836446	38.0	35.0	41.0	30.0	41.0
33	36.264348150620386	38.0	35.0	41.0	30.0	41.0
34	36.17205273412288	38.0	35.0	41.0	30.0	41.0
35	36.11729937938217	38.0	35.0	41.0	30.0	41.0
36	36.036584949867	38.0	35.0	41.0	30.0	41.0
37	36.01295144279099	38.0	35.0	41.0	30.0	41.0
38	35.96242850922633	38.0	35.0	41.0	30.0	41.0
39	35.8961557009022	38.0	35.0	41.0	30.0	41.0
40	35.76219080303092	38.0	35.0	40.0	29.0	41.0
41	35.743447863486814	38.0	35.0	40.0	29.0	41.0
42	35.71182103412227	38.0	35.0	40.0	29.0	41.0
43	35.62944520283615	38.0	35.0	40.0	29.0	41.0
44	35.50678773585852	38.0	34.0	40.0	29.0	41.0
45	35.40916299194113	37.0	34.0	40.0	28.0	41.0
46	35.402555508810984	37.0	34.0	40.0	28.0	41.0
47	35.39192360762292	37.0	34.0	40.0	28.0	41.0
48	35.32203638095327	37.0	34.0	40.0	28.0	41.0
49	35.25205417882007	37.0	34.0	40.0	28.0	41.0
50	35.171137759234526	37.0	34.0	40.0	28.0	41.0
51	34.815076084041245	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	6.0
13	12.0
14	16.0
15	48.0
16	94.0
17	249.0
18	490.0
19	951.0
20	1622.0
21	2751.0
22	4160.0
23	6167.0
24	9243.0
25	12961.0
26	17419.0
27	21564.0
28	24423.0
29	28478.0
30	33909.0
31	41488.0
32	53132.0
33	70175.0
34	124127.0
35	192525.0
36	99063.0
37	131430.0
38	204596.0
39	413724.0
40	290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.71414927066201	23.649224846383877	29.843698478319176	12.792927404634934
2	30.62196220645392	25.476031069015725	31.068413769301923	12.833592955228431
3	29.33190112117866	25.317181262009882	31.71397938497368	13.636938231837782
4	26.86942813400637	27.2119417599756	31.435875175487233	14.4827549305308
5	24.01849212405936	32.45238017206611	29.391896185129916	14.137231518744612
6	21.851446335853307	42.41523941508491	25.4628548955504	10.270459353511384
7	88.76982027565626	3.8502517853046205	5.580544209406183	1.7993837296329467
8	90.300731110417	2.727467907322675	5.345245842649735	1.6265551396105873
9	86.88823595115586	4.334693533575498	6.4492352803080415	2.327835234960602
10	45.25005634987891	28.105848147610597	13.833711340137233	12.810384162373262
11	38.77072321140134	25.80356265002946	21.033119014288456	14.392595124280744
12	36.135020329431086	22.85651414632776	24.88123050745658	16.12723501678457
13	24.954736165519492	31.277226020869186	26.121329148170418	17.6467086654409
14	17.31135164130309	33.99546391835321	29.530613869226812	19.16257057111689
15	17.389940493190192	27.405504430070305	38.62565153502421	16.5789035417153
16	21.580900032973876	24.394916003566262	37.01695445792754	17.00722950553232
17	22.97208992169875	24.143832982303127	26.78756195978525	26.096515136212872
18	24.07387218309129	26.77070715921031	30.928759707395308	18.226660950303085
19	27.780122438087034	27.902319742255322	25.303804436156756	19.01375338350089
20	29.230571665341248	27.546763711079294	25.909774647303262	17.3128899762762
21	25.887836652904145	27.286316911718966	29.585927044129477	17.239919391247412
22	23.791353621073316	22.42083092829152	29.66839517551399	24.11942027512118
23	20.767923441750277	27.294944964394237	30.155311636567696	21.781819957287794
24	20.46908515219149	25.623711226434214	34.71694302074144	19.190260600632858
25	20.852063676366424	27.261636668019957	31.90386342795877	19.982436227654848
26	19.33098480860772	32.8420471091676	26.70281976800571	21.124148314218964
27	18.560880944243383	31.785010330253765	30.979257224990853	18.674851500512
28	16.311099488135756	29.17846892864333	34.465926883607565	20.044504699613345
29	17.79485701176425	27.557598940020316	34.52291216174188	20.124631886473555
30	20.872864640568032	28.887589850467155	31.04079062391522	19.198754885049592
31	27.227458877965223	26.457488781859418	26.935108348945203	19.379943991230153
32	28.52889026521564	26.432340349255547	27.167129393367635	17.87163999216118
33	27.320093396998107	26.64971376936881	26.29623114620001	19.733961687433073
34	19.90886368546267	27.463693622531387	29.517638348149283	23.10980434385666
35	20.126103337317396	27.897972273853057	30.897257282511205	21.078667106318346
36	27.177095128628213	26.983599342662778	27.200303921483382	18.639001607225627
37	21.00195101005068	32.13688773432018	27.677990372698435	19.183170882930703
38	21.973978060667918	31.856175043792383	25.52525778815522	20.644589107384476
39	21.471477597495323	29.055469014923858	27.884729216258464	21.58832417132236
40	24.293987852504443	27.0226596741539	27.236755771933147	21.446596701408513
41	19.751685981688464	25.036201034965018	28.70238769653065	26.509725286815865
42	21.738412157394407	25.534688450381676	27.63177343937589	25.095125952848026
43	21.770115234666314	24.891463779234215	27.879779790692805	25.458641195406667
44	20.633887646701975	27.668359058084185	29.79935430061607	21.898398994597766
45	19.873348212822624	32.59718431192618	25.544587301512983	21.984880173738215
46	21.82161601420084	30.59152992763806	27.16672808859204	20.420125969569057
47	20.993322957375412	28.296401031888347	28.450970254621193	22.25930575611505
48	21.640360023891013	27.190003765576478	30.844017515615775	20.325618694916738
49	22.112628860635546	25.82884485089187	30.863213260715007	21.19531302775758
50	20.02410504018733	30.623768077944092	28.57544161918451	20.776685262684076
51	19.01589367563739	30.855053396944598	28.30904213231955	21.82001079509846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	796.0
1	1787.5
2	2779.0
3	6239.0
4	9699.0
5	7068.0
6	4437.0
7	4489.5
8	4542.0
9	4722.0
10	4902.0
11	4775.0
12	4648.0
13	4783.0
14	4918.0
15	4636.0
16	4354.0
17	4443.0
18	4532.0
19	4650.5
20	4769.0
21	5113.5
22	5458.0
23	6770.5
24	8083.0
25	9193.5
26	12569.5
27	14835.0
28	17218.0
29	19601.0
30	22614.5
31	25628.0
32	30817.5
33	36007.0
34	41244.5
35	46482.0
36	52498.0
37	58514.0
38	63293.0
39	68072.0
40	85820.0
41	103568.0
42	116701.5
43	129835.0
44	132831.0
45	135827.0
46	134522.0
47	133217.0
48	131079.5
49	128942.0
50	119021.0
51	109100.0
52	100016.0
53	90932.0
54	83233.0
55	75534.0
56	71478.0
57	67422.0
58	59449.5
59	51477.0
60	46072.5
61	40668.0
62	35322.0
63	29976.0
64	25203.5
65	20431.0
66	16765.0
67	13099.0
68	10425.0
69	7751.0
70	6695.5
71	5640.0
72	4807.0
73	3974.0
74	3196.0
75	1645.0
76	872.0
77	766.0
78	660.0
79	494.5
80	329.0
81	185.5
82	42.0
83	31.5
84	21.0
85	16.0
86	11.0
87	9.0
88	7.0
89	6.5
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1495123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.544851684600925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68099796895642	14.96217141099885
2	6.703828203075317	2.48642999490153
3	2.049844093161848	1.1404216405272523
4	1.0560651491071789	0.7833828623787435
5	0.7042876796265937	0.6530455280983456
6	0.4936933954719481	0.5493282478016585
7	0.41717190562526013	0.5415473781761948
8	0.3340686172643071	0.4956202367717029
9	0.25981372042927975	0.4336386219886826
>10	4.20818144909878	19.496148330677983
>50	1.8495578837322266	24.547058444698337
>100	1.2388547971353767	33.114654321699035
>500	0.002544596120876652	0.2914941681670668
>1k	7.270274631076149E-4	0.16516205180603924
>5k	3.6351373155380743E-4	0.33989676130859175
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5042	0.3372297797572507	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023075024596638537	0.0
2	0.0	0.0	0.0	0.08955784908666377	0.0
3	0.0	0.0	0.0	0.13457086808242533	0.0
4	0.0	0.0	0.0	0.2174403042425272	0.0
5	0.0	0.0	0.0	0.3936799848574331	0.0
6	0.0	0.0	0.0	0.5607565397629493	0.0
7	0.0	0.0	0.0	0.661550922566237	0.0
8	0.0	0.0	0.0	0.8590597562876098	0.0
9	0.0	0.0	0.0	0.9228003314777447	0.0
10	0.0	0.0	0.0	1.0546958343895452	0.0
11	0.0	0.0	0.0	1.26270547640562	0.0
12	0.0	0.0	0.0	1.4211539786358713	0.0
13	0.0	0.0	0.0	1.4818178838797877	0.0
14	0.0	0.0	0.0	1.509374145137223	0.0
15	0.0	0.0	0.0	1.5549222371671094	0.0
16	0.0	0.0	0.0	1.6552484310655378	0.0
17	0.0	0.0	0.0	1.7710917429535897	0.0
18	0.0	0.0	0.0	1.9187050162428108	0.0
19	0.0	0.0	0.0	2.013479827412193	0.0
20	0.0	0.0	0.0	2.125310091544308	0.0
21	0.0	0.0	0.0	2.280481271440544	0.0
22	0.0	0.0	0.0	2.427626355824905	0.0
23	0.0	0.0	0.0	2.580590359455376	0.0
24	0.0	0.0	0.0	2.6997778778067087	0.0
25	0.0	0.0	0.0	2.801909943195309	0.0
26	0.0	0.0	0.0	2.9081888245983776	0.0
27	0.0	0.0	0.0	3.0238314840986327	0.0
28	0.0	0.0	0.0	3.1464300930425124	0.0
29	0.0	0.0	0.0	3.2909666963855146	0.0
30	0.0	0.0	0.0	3.443261858723329	0.0
31	0.0	0.0	0.0	3.5825814999836134	0.0
32	0.0	0.0	0.0	3.704043078729977	0.0
33	0.0	0.0	0.0	3.8349353197027938	0.0
34	0.0	0.0	0.0	3.981679099311562	0.0
35	0.0	0.0	0.0	4.153236890877874	0.0
36	0.0	0.0	0.0	4.291352617811377	0.0
37	0.0	0.0	0.0	4.447995248551457	0.0
38	0.0	0.0	0.0	4.663094608269687	0.0
39	0.0	0.0	0.0	5.053764807310168	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	25	3.8913702E-5	45.000004	11
TCGATCA	50	2.1827873E-11	45.000004	17
CTAACCG	25	3.8913702E-5	45.000004	18
TTAAGCG	25	3.8913702E-5	45.000004	1
TACAGCG	65	0.0	45.000004	1
CGAACTA	40	6.8175723E-9	45.0	33
CCGATTA	20	7.033968E-4	45.0	10
ACCGCTA	20	7.033968E-4	45.0	17
CTACGCG	35	1.2122655E-7	45.0	1
CATACGT	20	7.033968E-4	45.0	11
CGCAATA	20	7.033968E-4	45.0	14
TATCGCG	75	0.0	45.0	1
TACGTTC	20	7.033968E-4	45.0	31
ATCGATA	20	7.033968E-4	45.0	36
GTTTACG	55	1.8189894E-12	44.999996	1
CGGTCTA	380	0.0	44.407894	31
CGACGGT	390	0.0	43.846153	28
GCGATAT	135	0.0	43.333336	9
AATAGCG	135	0.0	43.333336	1
TCACGAC	415	0.0	42.831326	25
>>END_MODULE