FastQCFastQC Report
Thu 26 May 2016
SRR1547040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547040_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1311746
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81840.6239012735697307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT502.1827873E-1145.027
CGTGCTA207.0336513E-445.020
ATAGCGC207.0336513E-445.035
CACGACG1950.045.026
TCGTCGA253.8911086E-545.044
ATACGCC207.0336513E-445.04
GCGTACC207.0336513E-445.030
TCACGAC2050.042.8048825
CGGTCTA2050.042.8048831
CGTTTTT45400.042.125551
TATAGCG1200.041.2500041
GGCGATA2650.040.754728
ACGGGTC1050.040.7142875
TGATTCG451.9286745E-840.015
CCCGATC1800.040.041
CTCACGA2200.039.88636424
TACGGGA4500.039.04
GCGATAT1100.038.8636369
ATAGGGA14800.038.7668954
ATTAGCG1750.038.5714261
Produced by FastQC (version 0.11.5)