##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547040_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1311746
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42119434707634	31.0	31.0	33.0	30.0	34.0
2	31.82936940535744	31.0	31.0	34.0	30.0	34.0
3	31.951652225354604	33.0	31.0	34.0	30.0	34.0
4	35.70006540900449	37.0	35.0	37.0	33.0	37.0
5	35.604816023833884	37.0	35.0	37.0	33.0	37.0
6	35.66463858094479	37.0	35.0	37.0	33.0	37.0
7	36.00551097544799	37.0	35.0	37.0	35.0	37.0
8	35.991043235504435	37.0	35.0	37.0	35.0	37.0
9	37.771197320212906	39.0	38.0	39.0	35.0	39.0
10	37.19714258705572	39.0	37.0	39.0	33.0	39.0
11	36.90182169413896	39.0	37.0	39.0	33.0	39.0
12	36.33120360191683	38.0	35.0	39.0	32.0	39.0
13	36.12042346612835	38.0	35.0	39.0	32.0	39.0
14	37.07906103773139	39.0	35.0	41.0	31.0	41.0
15	37.3347279122635	39.0	35.0	41.0	32.0	41.0
16	37.417489361507485	39.0	35.0	41.0	32.0	41.0
17	37.34509196140107	39.0	35.0	41.0	32.0	41.0
18	37.25396532560419	39.0	35.0	41.0	32.0	41.0
19	37.21859186153417	39.0	35.0	41.0	32.0	41.0
20	37.06527940622651	39.0	35.0	41.0	32.0	41.0
21	36.96319409397856	39.0	35.0	41.0	32.0	41.0
22	36.882927030080516	39.0	35.0	41.0	31.0	41.0
23	36.840936431290814	38.0	35.0	41.0	31.0	41.0
24	36.76110314039456	38.0	35.0	41.0	31.0	41.0
25	36.70873400795581	38.0	35.0	41.0	31.0	41.0
26	36.58683769571243	38.0	35.0	41.0	31.0	41.0
27	36.4736275163027	38.0	35.0	40.0	31.0	41.0
28	36.414208238485195	38.0	35.0	40.0	31.0	41.0
29	36.45151500366687	38.0	35.0	40.0	31.0	41.0
30	36.42469731182714	38.0	35.0	40.0	31.0	41.0
31	36.32237338631107	38.0	35.0	40.0	30.0	41.0
32	36.23766110207311	38.0	35.0	41.0	30.0	41.0
33	36.138365201799736	38.0	35.0	41.0	30.0	41.0
34	36.01408046984706	38.0	35.0	41.0	30.0	41.0
35	35.94254375465982	38.0	35.0	41.0	30.0	41.0
36	35.835526085080495	38.0	35.0	41.0	29.0	41.0
37	35.810441960562486	38.0	35.0	41.0	29.0	41.0
38	35.75369774331311	38.0	35.0	41.0	29.0	41.0
39	35.685890408661436	38.0	35.0	40.0	29.0	41.0
40	35.55317264165471	38.0	35.0	40.0	28.0	41.0
41	35.504479525761845	38.0	34.0	40.0	28.0	41.0
42	35.47150439185635	38.0	34.0	40.0	28.0	41.0
43	35.39283748530585	38.0	34.0	40.0	27.0	41.0
44	35.25278293206154	38.0	34.0	40.0	27.0	41.0
45	35.170744946049005	37.0	34.0	40.0	27.0	41.0
46	35.15895302901629	37.0	34.0	40.0	27.0	41.0
47	35.143083340829705	37.0	34.0	40.0	27.0	41.0
48	35.06006269506444	37.0	34.0	40.0	27.0	41.0
49	35.00397866660161	37.0	34.0	40.0	27.0	41.0
50	34.93547531305603	37.0	34.0	40.0	27.0	41.0
51	34.570461811966645	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	2.0
13	6.0
14	19.0
15	45.0
16	109.0
17	246.0
18	566.0
19	1065.0
20	1808.0
21	2768.0
22	4192.0
23	6315.0
24	8998.0
25	13122.0
26	17641.0
27	20724.0
28	22969.0
29	25749.0
30	30273.0
31	37051.0
32	46513.0
33	60835.0
34	105359.0
35	155201.0
36	90345.0
37	118148.0
38	182301.0
39	359096.0
40	270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.235896278700295	23.214021616989875	28.994561447109422	13.555520657200404
2	32.161409297226754	24.9287590737841	29.473846308660367	13.435985320328783
3	30.16208930692375	24.432397735537215	31.998725362989482	13.406787594549554
4	27.178279941391093	27.434427091830276	30.493555917075412	14.89373704970322
5	23.893345205550464	31.321307631202995	29.816443122372778	14.968904040873769
6	22.410817338112714	41.01838313210027	26.474790088934903	10.096009440852116
7	86.45599071771517	4.269576579612211	7.2292196812492655	2.045213021423355
8	87.03377025735165	3.6437694492683796	7.23966377637134	2.082796517008628
9	82.9900758226059	5.423839676278792	8.58565606451249	3.0004284366028178
10	44.93987403049066	29.63195618663979	14.005760261514045	11.422409521355506
11	36.3388948775144	24.384980018997577	24.328795361297082	14.947329742190943
12	34.52817847357644	21.918572650497886	27.143745816644383	16.409503059281295
13	24.466703157471034	29.428410683165794	27.92804399632246	18.17684216304071
14	17.49111489571914	32.5047684536488	31.12950220545746	18.8746144451746
15	17.428755262070553	27.56097598163059	38.43419381496113	16.57607494133773
16	22.935842762242082	24.15566733193774	36.403007899395156	16.505482006425023
17	22.447790959530277	24.531959693416255	27.899074973356125	25.121174373697347
18	23.756275986357114	26.851616090310166	31.528359911141337	17.863748012191387
19	27.075744846944456	27.420933625869644	26.881347455986145	18.62197407119976
20	27.992233252474186	27.121028003897095	27.424211699521095	17.462527044107627
21	25.805376955599634	27.209993398112136	30.10575218068132	16.87887746560691
22	23.839828747333705	22.647448515185104	30.63466555262985	22.878057184851336
23	21.509270849691937	26.446049768781453	30.72187755861272	21.32280182291389
24	20.42331365980914	25.88748126542791	34.52398558867342	19.165219486089534
25	21.545253425586967	26.82897451183385	31.443663636100283	20.182108426478905
26	19.57314907001813	31.894894285936452	27.35491474721478	21.177041896830637
27	18.273659687165043	31.399371524670173	31.157937588527044	19.169031199637736
28	17.280098433690668	28.34230102474107	35.1259313922055	19.25166914936276
29	18.629063858399416	28.173442114555712	33.49070628002677	19.706787747018094
30	20.79777639878452	28.880819914831076	31.557252699836706	18.7641509865477
31	26.373398508552725	26.482032344676487	28.193186790735403	18.95138235603539
32	27.008125048599346	27.407440159909008	27.234693301904482	18.34974148958716
33	26.290455621743842	27.164786475430457	26.970846490097934	19.573911412727767
34	21.036694603985833	26.901702006333544	29.711392296984325	22.3502110926963
35	20.976393295653274	28.216209540566545	30.34306946619239	20.464327697587795
36	26.98815166960677	27.31611150329408	27.018111738095634	18.67762508900351
37	21.786001253291413	30.886543583895055	28.243348941029744	19.084106221783788
38	21.58077859585621	31.064093200970312	26.592724506116276	20.762403697057206
39	21.38432287958187	30.045450872348763	27.118893444310103	21.451332803759264
40	24.435599574917706	26.540275327693013	27.95998615585639	21.06413894153289
41	19.58770981577226	25.488623559744035	29.09084533133701	25.83282129314669
42	21.308622248514574	26.155520962137484	27.631950087898115	24.903906701449824
43	21.421372735270396	25.834650915649824	27.835190654288255	24.908785694791522
44	19.95554017317377	28.118248502377746	29.911812195348798	22.014399129099687
45	20.345173532070994	32.17650368287763	25.945800482715402	21.53252230233597
46	22.0722609407614	30.660890141841485	27.317255017358544	19.949593900038575
47	20.918836421075422	28.05954811373543	28.78850021269362	22.233115252495526
48	21.239401530479224	27.61251034880228	30.95545936484655	20.192628755871944
49	21.955851208999302	26.424856641453452	30.958737438498	20.660554711049244
50	20.19011302493013	29.878497818937504	29.004776839418607	20.926612316713754
51	20.141780497138928	30.466492750883173	27.836410402623677	21.55531634935422
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1152.0
1	2098.0
2	3044.0
3	8569.0
4	14094.0
5	9913.0
6	5732.0
7	5641.5
8	5551.0
9	5678.0
10	5805.0
11	5763.5
12	5722.0
13	5534.5
14	5347.0
15	5102.0
16	4857.0
17	5034.5
18	5212.0
19	5097.5
20	4983.0
21	5288.5
22	5594.0
23	6217.5
24	6841.0
25	8846.0
26	12421.5
27	13992.0
28	17225.5
29	20459.0
30	23430.0
31	26401.0
32	31309.0
33	36217.0
34	39985.5
35	43754.0
36	48322.5
37	52891.0
38	56507.5
39	60124.0
40	72179.0
41	84234.0
42	91589.0
43	98944.0
44	102803.0
45	106662.0
46	108540.5
47	110419.0
48	106355.0
49	102291.0
50	95583.0
51	88875.0
52	81654.0
53	74433.0
54	68186.0
55	61939.0
56	59129.0
57	56319.0
58	53730.0
59	51141.0
60	46435.0
61	41729.0
62	36960.0
63	32191.0
64	28029.0
65	23867.0
66	19908.0
67	15949.0
68	12846.0
69	9743.0
70	8008.0
71	6273.0
72	4934.5
73	3596.0
74	2958.0
75	1776.0
76	1232.0
77	912.5
78	593.0
79	417.5
80	242.0
81	149.5
82	57.0
83	54.5
84	52.0
85	31.0
86	10.0
87	8.0
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1311746.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.51980775094073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.79458231666995	17.171640710213516
2	6.1609420196271065	2.6516457565413565
3	2.061935684393219	1.331173785688394
4	1.1593783375876612	0.997983957419669
5	0.8006157636872031	0.86145486584606
6	0.6109288968894281	0.788824344633287
7	0.5159943795910312	0.7772869894555448
8	0.42974379215499225	0.739840303146853
9	0.35477427792898486	0.6871206830409511
>10	5.808309494459087	30.955502685684483
>50	1.751537174338972	26.783807423103124
>100	0.547333049410692	15.07671290758989
>500	0.002854409644905793	0.38998051925160215
>1k	7.136024112264482E-4	0.1586335406130754
>5k	3.568012056132241E-4	0.6283915277722213
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8184	0.6239012735697307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029350194321156688	0.0
2	0.0	0.0	0.0	0.09414932464059353	0.0
3	0.0	0.0	0.0	0.13813649898684655	0.0
4	0.0	0.0	0.0	0.2113976333832922	0.0
5	0.0	0.0	0.0	0.3892521875424053	0.0
6	0.0	0.0	0.0	0.5570438179342647	0.0
7	0.0	0.0	0.0	0.6740634238640713	0.0
8	0.0	0.0	0.0	0.8732635738931165	0.0
9	0.0	0.0	0.0	0.9538431983021103	0.0
10	0.0	0.0	0.0	1.0981546732370444	0.0
11	0.0	0.0	0.0	1.3022338166077885	0.0
12	0.0	0.0	0.0	1.4624782541742074	0.0
13	0.0	0.0	0.0	1.5262863389711119	0.0
14	0.0	0.0	0.0	1.5587621384017942	0.0
15	0.0	0.0	0.0	1.6017582672255146	0.0
16	0.0	0.0	0.0	1.7003291795820228	0.0
17	0.0	0.0	0.0	1.8156716315506203	0.0
18	0.0	0.0	0.0	1.9535031934536107	0.0
19	0.0	0.0	0.0	2.0433071646492538	0.0
20	0.0	0.0	0.0	2.1397435174187684	0.0
21	0.0	0.0	0.0	2.275592988276694	0.0
22	0.0	0.0	0.0	2.416702623831138	0.0
23	0.0	0.0	0.0	2.560556693140288	0.0
24	0.0	0.0	0.0	2.675136802399245	0.0
25	0.0	0.0	0.0	2.777138256949135	0.0
26	0.0	0.0	0.0	2.8754042322217868	0.0
27	0.0	0.0	0.0	2.990213044293636	0.0
28	0.0	0.0	0.0	3.109290975539472	0.0
29	0.0	0.0	0.0	3.252077765055125	0.0
30	0.0	0.0	0.0	3.401573170415614	0.0
31	0.0	0.0	0.0	3.5326198822028045	0.0
32	0.0	0.0	0.0	3.6574916180419077	0.0
33	7.623427096404335E-5	0.0	0.0	3.7875472843065654	0.0
34	7.623427096404335E-5	0.0	0.0	3.9312488850737872	0.0
35	7.623427096404335E-5	0.0	0.0	4.1043769144331295	0.0
36	7.623427096404335E-5	0.0	0.0	4.243961864568293	0.0
37	7.623427096404335E-5	0.0	0.0	4.399098605980122	0.0
38	7.623427096404335E-5	0.0	0.0	4.595249385170605	0.0
39	7.623427096404335E-5	0.0	0.0	4.952025773282328	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAT	50	2.1827873E-11	45.0	27
CGTGCTA	20	7.0336513E-4	45.0	20
ATAGCGC	20	7.0336513E-4	45.0	35
CACGACG	195	0.0	45.0	26
TCGTCGA	25	3.8911086E-5	45.0	44
ATACGCC	20	7.0336513E-4	45.0	4
GCGTACC	20	7.0336513E-4	45.0	30
TCACGAC	205	0.0	42.80488	25
CGGTCTA	205	0.0	42.80488	31
CGTTTTT	4540	0.0	42.12555	1
TATAGCG	120	0.0	41.250004	1
GGCGATA	265	0.0	40.75472	8
ACGGGTC	105	0.0	40.714287	5
TGATTCG	45	1.9286745E-8	40.0	15
CCCGATC	180	0.0	40.0	41
CTCACGA	220	0.0	39.886364	24
TACGGGA	450	0.0	39.0	4
GCGATAT	110	0.0	38.863636	9
ATAGGGA	1480	0.0	38.766895	4
ATTAGCG	175	0.0	38.571426	1
>>END_MODULE