FastQCFastQC Report
Thu 26 May 2016
SRR1547039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547039_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1209159
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52380.43319364947041705No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14030.11603105960423733No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13850.11454242163354861No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13220.10933218873613809No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG12230.10114467989735014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCG351.2119926E-745.00000424
TCGTCGA351.2119926E-745.00000432
TCCAATC351.2119926E-745.00000423
CGTCGAC351.2119926E-745.00000433
ATACGAG551.8189894E-1245.0000041
TCACGAG207.0334325E-445.01
GCCCCTA207.0334325E-445.041
CTTAACG253.8909264E-545.01
ATGGGCG2250.045.05
TAATACG406.8157533E-945.01
AACCGAT207.0334325E-445.041
ACGCCGG453.8562575E-1045.027
CCGATTC253.8909264E-545.021
ACGTTAT207.0334325E-445.015
CGTGCAA207.0334325E-445.030
GCGCGAT253.8909264E-545.011
GAATCGT207.0334325E-445.016
CACGATA253.8909264E-545.030
ATATGCG800.045.01
TGACGTA406.8157533E-945.044