##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547039_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1209159
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42119109232119	31.0	31.0	33.0	30.0	34.0
2	31.826251138187782	31.0	31.0	34.0	30.0	34.0
3	31.94230452736158	33.0	31.0	34.0	30.0	34.0
4	35.666015801065036	37.0	35.0	37.0	33.0	37.0
5	35.55084649744161	37.0	35.0	37.0	33.0	37.0
6	35.62177678866055	37.0	35.0	37.0	33.0	37.0
7	36.01312730583819	37.0	35.0	37.0	35.0	37.0
8	36.02378181860285	37.0	35.0	37.0	35.0	37.0
9	37.86570996866417	39.0	38.0	39.0	35.0	39.0
10	37.24658543665473	39.0	37.0	39.0	34.0	39.0
11	36.86140118876012	39.0	35.0	39.0	33.0	39.0
12	35.724279437195605	37.0	35.0	39.0	32.0	39.0
13	35.17466602820638	37.0	35.0	39.0	30.0	39.0
14	35.8703412867952	38.0	35.0	41.0	27.0	41.0
15	36.38145934488351	38.0	35.0	41.0	31.0	41.0
16	36.61820240348871	38.0	35.0	41.0	32.0	41.0
17	36.61766483977707	38.0	35.0	41.0	32.0	41.0
18	36.57577043217641	37.0	35.0	40.0	32.0	41.0
19	36.51655324072351	37.0	35.0	40.0	31.0	41.0
20	36.313913223984606	37.0	35.0	40.0	31.0	41.0
21	36.17025717874986	37.0	35.0	40.0	31.0	41.0
22	36.10974983438903	36.0	35.0	40.0	31.0	41.0
23	36.16666377209283	36.0	35.0	40.0	31.0	41.0
24	36.07256283086013	36.0	35.0	40.0	31.0	41.0
25	35.98848621231782	36.0	35.0	40.0	31.0	41.0
26	35.85789958144463	36.0	35.0	40.0	31.0	41.0
27	35.82585664912555	36.0	34.0	40.0	30.0	41.0
28	35.82224670204663	36.0	35.0	40.0	30.0	41.0
29	35.90685344111072	36.0	35.0	40.0	31.0	41.0
30	35.89815235217205	36.0	35.0	40.0	31.0	41.0
31	35.70599482781007	36.0	34.0	40.0	30.0	41.0
32	35.60357570840559	36.0	34.0	40.0	30.0	41.0
33	35.52338857007226	36.0	34.0	40.0	30.0	41.0
34	35.42614825676358	36.0	34.0	40.0	30.0	41.0
35	35.370294560103346	36.0	34.0	40.0	29.0	41.0
36	35.24331870333017	36.0	34.0	40.0	29.0	41.0
37	35.19581874674877	36.0	34.0	40.0	29.0	41.0
38	35.16018819692034	35.0	34.0	40.0	29.0	41.0
39	35.14448637441395	36.0	34.0	40.0	29.0	41.0
40	34.962638495020094	36.0	34.0	40.0	27.0	41.0
41	34.94296614423744	36.0	34.0	40.0	27.0	41.0
42	34.927694372700365	36.0	34.0	40.0	27.0	41.0
43	34.83301782478566	35.0	34.0	40.0	27.0	41.0
44	34.74874024011731	35.0	34.0	40.0	27.0	41.0
45	34.6522401106885	35.0	34.0	40.0	27.0	41.0
46	34.67832187495606	35.0	34.0	40.0	27.0	41.0
47	34.65165540677446	35.0	34.0	40.0	27.0	41.0
48	34.6249508956225	35.0	34.0	40.0	27.0	41.0
49	34.614939805269614	35.0	34.0	40.0	27.0	41.0
50	34.47474649735891	35.0	34.0	40.0	27.0	41.0
51	34.1037903203797	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	8.0
13	7.0
14	12.0
15	31.0
16	104.0
17	244.0
18	525.0
19	1038.0
20	1734.0
21	2871.0
22	4395.0
23	6242.0
24	8922.0
25	12806.0
26	17096.0
27	20392.0
28	22981.0
29	25993.0
30	30933.0
31	38194.0
32	49146.0
33	66007.0
34	125440.0
35	214173.0
36	70036.0
37	87955.0
38	136873.0
39	264778.0
40	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.19444258364698	24.05175828819866	30.49921474347046	13.254584384683902
2	30.01755765784318	25.634842067916626	31.514713945808616	12.832886328431577
3	29.530359530880556	25.371601253433173	32.26250641975125	12.835532795935025
4	27.34412926670521	26.73924603794869	31.279178338001866	14.637446357344237
5	23.792073664422958	31.73387453593779	30.196111512216344	14.277940287422911
6	21.513713250283875	40.727728942182125	27.006704660015764	10.751853147518235
7	89.31736851811878	3.7561644084855668	5.704295299460203	1.2221717739354379
8	91.11704912257197	2.265541587169264	5.228840872044123	1.3885684182146434
9	88.59868718671407	3.3274366729272162	6.04734364959447	2.026532490764242
10	55.92250481533032	21.99727248442926	11.637840846406469	10.442381853833945
11	50.493855646775984	18.785039850011454	18.392039425749633	12.32906507746293
12	45.04064395170528	19.949320147308995	21.662577047352745	13.347458853632979
13	23.72864114644972	39.03308001677199	23.246157039727613	13.992121797050677
14	14.831796314628598	42.191142769478624	28.676708356800056	14.300352559092724
15	13.623435792976771	24.03190978192281	48.249154991196356	14.09549943390406
16	18.995930229192357	19.00742582241045	45.974516171984	16.022127776413193
17	20.031526044134807	18.690676743091686	26.236665318622283	35.04113189415122
18	25.642616066208003	23.10763100634408	33.069100093536086	18.180652833911832
19	31.57723674057754	25.80611813665531	23.81010272429019	18.806542398476957
20	32.57189501132605	25.733588386638978	23.6895230486644	18.004993553370564
21	24.197810213545118	27.214204252707873	29.083106522798076	19.504879010948933
22	22.01257237468356	21.267426368244376	29.488843071920236	27.231158185151827
23	19.42904117655329	27.935283945287594	28.035932412528048	24.59974246563107
24	20.184028734020917	23.601362599955834	38.28330269220177	17.931305973821473
25	19.65597576497384	23.568364458272235	35.58374043446726	21.191919342286663
26	16.457306276511193	35.398653113444965	26.046119658374128	22.097920951669714
27	16.206884288997557	35.06594252699603	31.52703656012154	17.200136623884866
28	14.143135848966098	29.077234673024805	39.56229081535182	17.21733866265727
29	16.21796637166824	26.613208023097044	37.89220441645805	19.276621188776662
30	20.175923927291613	29.856950161227765	32.7500353551518	17.21709055632882
31	32.06732944137206	23.540493847376563	26.233853446899868	18.158323264351502
32	32.40425783540461	25.384171974074544	26.16752635509474	16.044043835426113
33	30.150708054110336	26.124934768711146	24.364868474700184	19.359488702478334
34	19.553590553434248	27.66757721689207	28.592104098799247	24.186728130874435
35	19.677064802891927	27.624241311523136	31.764308912227424	20.934384973357513
36	31.853875296797195	24.0348043557547	27.37390202611898	16.737418321329123
37	20.443630655687134	33.456476774353085	28.65297285137852	17.44691971858126
38	20.982352196857484	34.22213290394398	22.75622974315206	22.039285156046475
39	20.634589826482706	30.42610607868775	26.836255612371907	22.103048482457645
40	25.467783806761556	25.620038390319223	25.52005153995463	23.392126262964588
41	17.964634923943006	23.38476577522063	28.608975329133717	30.04162397170265
42	22.7574702747943	23.29949990034396	25.497804672503783	28.44522515235796
43	22.616215071797836	22.46205833972207	27.495722233387003	27.426004355093088
44	19.889774628481447	28.492944269529485	30.19420936369824	21.423071738290826
45	17.569815053272563	37.95729097662094	22.21750820198171	22.25538576812479
46	23.559432630448104	31.223602520429488	25.71076260442175	19.506202244700653
47	20.41724868276215	27.341648203420725	27.431214587990493	24.80988852582663
48	22.95860180505624	24.753651091378387	32.00712230566865	20.28062479789672
49	22.740929852897757	22.256212789219614	32.73804354927681	22.264813808605815
50	19.661185997871247	31.82550847324463	27.886241594364346	20.627063934519775
51	18.127723483842903	34.965624868193515	25.873272249555267	21.033379398408314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1185.0
1	1746.0
2	2307.0
3	5384.5
4	8462.0
5	6062.5
6	3663.0
7	3833.5
8	4004.0
9	4341.5
10	4679.0
11	4576.0
12	4473.0
13	4361.0
14	4249.0
15	3831.0
16	3413.0
17	3288.5
18	3164.0
19	3224.0
20	3284.0
21	3286.0
22	3288.0
23	4013.0
24	4738.0
25	4928.0
26	6409.0
27	7700.0
28	9249.0
29	10798.0
30	12412.0
31	14026.0
32	16250.0
33	18474.0
34	21866.0
35	25258.0
36	27552.0
37	29846.0
38	37298.5
39	44751.0
40	64272.0
41	83793.0
42	107849.5
43	131906.0
44	134832.5
45	137759.0
46	129610.5
47	121462.0
48	112781.0
49	104100.0
50	99958.0
51	95816.0
52	84542.5
53	73269.0
54	68632.5
55	63996.0
56	58130.5
57	52265.0
58	49181.5
59	46098.0
60	38363.0
61	30628.0
62	26791.0
63	22954.0
64	19170.0
65	15386.0
66	12052.0
67	8718.0
68	7262.0
69	5806.0
70	4704.5
71	3603.0
72	3151.0
73	2699.0
74	1832.0
75	750.5
76	536.0
77	423.5
78	311.0
79	206.0
80	101.0
81	70.5
82	40.0
83	40.0
84	40.0
85	26.0
86	12.0
87	10.5
88	9.0
89	7.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1209159.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.782288431964265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.879980989285	14.382311503232522
2	7.5632396916883895	2.6898341935536685
3	2.3787985311806095	1.269014448089596
4	1.2229121625427875	0.8698470720517205
5	0.7223058855702301	0.6422125796657603
6	0.5288464603343015	0.5642460176332739
7	0.3914171284722237	0.4872204592993005
8	0.31669098734409473	0.4505192384604989
9	0.2516764626661773	0.4027845105599806
>10	2.814771476732659	12.145653392694697
>50	1.3569830450268918	17.65031384253608
>100	1.5611392243324405	46.5586697542643
>500	0.00889671423584774	1.00717623251189
>1k	0.0018729924707047876	0.4440534761893113
>5k	4.682481176761969E-4	0.43614327925738094
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5238	0.43319364947041705	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1403	0.11603105960423733	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1385	0.11454242163354861	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1322	0.10933218873613809	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1223	0.10114467989735014	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6540421896541315E-4	0.0	0.0	0.036388928172390894	0.0
2	1.6540421896541315E-4	0.0	0.0	0.12016616507837266	0.0
3	1.6540421896541315E-4	0.0	0.0	0.17632089741713042	0.0
4	1.6540421896541315E-4	0.0	0.0	0.2868936177955091	0.0
5	1.6540421896541315E-4	0.0	0.0	0.5024980172169251	0.0
6	1.6540421896541315E-4	0.0	0.0	0.6961863576254239	0.0
7	1.6540421896541315E-4	0.0	0.0	0.8148638847331079	0.0
8	1.6540421896541315E-4	0.0	0.0	1.0726463599907043	0.0
9	1.6540421896541315E-4	0.0	0.0	1.1545214483785837	0.0
10	1.6540421896541315E-4	0.0	0.0	1.3211661989862375	0.0
11	1.6540421896541315E-4	0.0	0.0	1.5599271890628115	0.0
12	1.6540421896541315E-4	0.0	0.0	1.7533674231428622	0.0
13	1.6540421896541315E-4	0.0	0.0	1.832017129260916	0.0
14	1.6540421896541315E-4	0.0	0.0	1.8629477182074483	0.0
15	1.6540421896541315E-4	0.0	0.0	1.9113284522548317	0.0
16	1.6540421896541315E-4	0.0	0.0	2.0165255355168346	0.0
17	1.6540421896541315E-4	0.0	0.0	2.138180338565896	0.0
18	1.6540421896541315E-4	0.0	0.0	2.3045769828451013	0.0
19	1.6540421896541315E-4	0.0	0.0	2.4018346635967642	0.0
20	1.6540421896541315E-4	0.0	0.0	2.5153019578070377	0.0
21	1.6540421896541315E-4	0.0	0.0	2.662594414795738	0.0
22	2.481063284481197E-4	0.0	0.0	2.8113755097551274	0.0
23	2.481063284481197E-4	0.0	0.0	2.970328964180889	0.0
24	2.481063284481197E-4	0.0	0.0	3.0833827478437494	0.0
25	2.481063284481197E-4	0.0	0.0	3.185519853054892	0.0
26	3.308084379308263E-4	0.0	0.0	3.2936115101487893	0.0
27	3.308084379308263E-4	0.0	0.0	3.4404077544805935	0.0
28	3.308084379308263E-4	0.0	0.0	3.558175558383968	0.0
29	3.308084379308263E-4	0.0	0.0	3.7273013722761026	0.0
30	3.308084379308263E-4	0.0	0.0	3.9291772215233896	0.0
31	3.308084379308263E-4	0.0	0.0	4.098881950181903	0.0
32	3.308084379308263E-4	0.0	0.0	4.23749068567492	0.0
33	3.308084379308263E-4	0.0	0.0	4.379490207656727	0.0
34	3.308084379308263E-4	0.0	0.0	4.557051636716098	0.0
35	4.1351054741353287E-4	0.0	0.0	4.761325847138383	0.0
36	4.1351054741353287E-4	0.0	0.0	4.906716155608981	0.0
37	4.1351054741353287E-4	0.0	0.0	5.081300308726974	0.0
38	4.1351054741353287E-4	0.0	0.0	5.3740657762957555	0.0
39	4.1351054741353287E-4	0.0	0.0	6.007977445480702	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCG	35	1.2119926E-7	45.000004	24
TCGTCGA	35	1.2119926E-7	45.000004	32
TCCAATC	35	1.2119926E-7	45.000004	23
CGTCGAC	35	1.2119926E-7	45.000004	33
ATACGAG	55	1.8189894E-12	45.000004	1
TCACGAG	20	7.0334325E-4	45.0	1
GCCCCTA	20	7.0334325E-4	45.0	41
CTTAACG	25	3.8909264E-5	45.0	1
ATGGGCG	225	0.0	45.0	5
TAATACG	40	6.8157533E-9	45.0	1
AACCGAT	20	7.0334325E-4	45.0	41
ACGCCGG	45	3.8562575E-10	45.0	27
CCGATTC	25	3.8909264E-5	45.0	21
ACGTTAT	20	7.0334325E-4	45.0	15
CGTGCAA	20	7.0334325E-4	45.0	30
GCGCGAT	25	3.8909264E-5	45.0	11
GAATCGT	20	7.0334325E-4	45.0	16
CACGATA	25	3.8909264E-5	45.0	30
ATATGCG	80	0.0	45.0	1
TGACGTA	40	6.8157533E-9	45.0	44
>>END_MODULE