FastQCFastQC Report
Thu 26 May 2016
SRR1547038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547038_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1073789
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52100.4851977436907996No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11580.10784241596812781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG302.1653668E-645.0000041
CGCTAAC302.1653668E-645.00000435
TCGCTAA302.1653668E-645.00000434
ATACGTA302.1653668E-645.00000437
TCACGAC1750.045.025
AACGTAC253.8906335E-545.037
CTATGCG207.033079E-445.01
ATATGCG551.8189894E-1245.01
CGCTATC207.033079E-445.041
ATATACG351.211829E-745.01
CACGTAT253.8906335E-545.042
CCCGGTC207.033079E-445.044
TAATGCG406.8139343E-945.01
CCGTTCG207.033079E-445.032
AAGTCGC207.033079E-445.040
CTCACGA1800.043.7524
CGGGATA1550.043.5483866
GGCGATA2200.041.931828
CATACGA3400.041.69117718
ATAGCGG1350.041.6666642
Produced by FastQC (version 0.11.5)