##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547038_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1073789
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42059287252896	31.0	31.0	33.0	30.0	34.0
2	31.84091101696888	31.0	31.0	34.0	30.0	34.0
3	31.942480319690368	33.0	31.0	34.0	30.0	34.0
4	35.68044746221092	37.0	35.0	37.0	33.0	37.0
5	35.58224660524554	37.0	35.0	37.0	33.0	37.0
6	35.63907248072014	37.0	35.0	37.0	33.0	37.0
7	35.992474312923676	37.0	35.0	37.0	35.0	37.0
8	35.98572810859489	37.0	35.0	37.0	35.0	37.0
9	37.77571012554608	39.0	38.0	39.0	35.0	39.0
10	37.20290392246521	39.0	37.0	39.0	33.0	39.0
11	36.83672118079064	39.0	35.0	39.0	33.0	39.0
12	35.98086216193312	37.0	35.0	39.0	32.0	39.0
13	35.599638290204126	37.0	35.0	39.0	31.0	39.0
14	36.44800235428003	38.0	35.0	41.0	31.0	41.0
15	36.81736542281584	38.0	35.0	41.0	31.0	41.0
16	36.9840545954559	38.0	35.0	41.0	32.0	41.0
17	36.94659285949102	38.0	35.0	41.0	32.0	41.0
18	36.82749031699896	38.0	35.0	41.0	32.0	41.0
19	36.772434807955754	38.0	35.0	41.0	32.0	41.0
20	36.58686483098635	38.0	35.0	41.0	31.0	41.0
21	36.469738468172054	38.0	35.0	41.0	31.0	41.0
22	36.4073649478622	38.0	35.0	40.0	31.0	41.0
23	36.416606055752105	38.0	35.0	40.0	31.0	41.0
24	36.30105821534771	38.0	35.0	40.0	31.0	41.0
25	36.22351039170638	37.0	35.0	40.0	31.0	41.0
26	36.07993097340353	37.0	35.0	40.0	31.0	41.0
27	35.972745111004116	37.0	35.0	40.0	30.0	41.0
28	35.92614377685001	37.0	35.0	40.0	30.0	41.0
29	35.970330297665555	37.0	35.0	40.0	30.0	41.0
30	35.95191979057338	37.0	35.0	40.0	30.0	41.0
31	35.80318945342148	37.0	35.0	40.0	30.0	41.0
32	35.682940503208734	37.0	34.0	40.0	30.0	41.0
33	35.54938819451494	37.0	34.0	40.0	29.0	41.0
34	35.42618335632047	37.0	34.0	40.0	29.0	41.0
35	35.347432316777315	37.0	34.0	40.0	29.0	41.0
36	35.22199519644921	37.0	34.0	40.0	27.0	41.0
37	35.17524951363816	37.0	34.0	40.0	27.0	41.0
38	35.12146334149446	37.0	34.0	40.0	27.0	41.0
39	35.06649909805371	37.0	34.0	40.0	27.0	41.0
40	34.898413934208676	36.0	34.0	40.0	26.0	41.0
41	34.8543186789956	36.0	34.0	40.0	26.0	41.0
42	34.80481733375924	36.0	34.0	40.0	26.0	41.0
43	34.71667059357099	36.0	34.0	40.0	26.0	41.0
44	34.62368118876241	36.0	34.0	40.0	26.0	41.0
45	34.53886936819058	35.0	34.0	40.0	25.0	41.0
46	34.538156006440744	35.0	34.0	40.0	25.0	41.0
47	34.495623441849375	35.0	34.0	40.0	25.0	41.0
48	34.42644877159293	35.0	34.0	40.0	25.0	41.0
49	34.380333566464174	36.0	34.0	40.0	25.0	41.0
50	34.29275677065047	35.0	34.0	40.0	24.0	41.0
51	33.942823031340424	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	2.0
13	8.0
14	8.0
15	52.0
16	99.0
17	253.0
18	617.0
19	1064.0
20	1825.0
21	2781.0
22	4373.0
23	6156.0
24	8976.0
25	12936.0
26	16733.0
27	19379.0
28	21400.0
29	23339.0
30	27430.0
31	33102.0
32	41342.0
33	54654.0
34	99739.0
35	157377.0
36	68209.0
37	89051.0
38	133402.0
39	249294.0
40	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.19969472587259	24.676356341888397	29.296910286844064	15.827038645394953
2	31.649514010666902	26.193972931367338	28.52050076877301	13.636012289192756
3	28.926260187057235	24.90982865348779	31.956930085892104	14.206981073562869
4	26.969451167780633	27.519000474022366	30.69224959465966	14.819298763537342
5	23.93030660586018	30.891357613087862	30.36797732142907	14.810358459622886
6	23.43952117222285	39.48978803098188	26.971313731096146	10.099377065699128
7	86.27290836467873	4.151839886607146	7.731220938191767	1.8440308105223653
8	87.37172759266485	3.1936441889421476	7.296498660351336	2.1381295580416637
9	83.76515311667376	4.758476758469308	8.379299843824066	3.097070281032866
10	51.59728773529996	25.94830083005134	13.358117842518407	9.096293592130298
11	44.85499478947912	20.003184983269527	21.803911196706245	13.337909030545106
12	40.15015985449655	20.357723910377178	24.945589869145614	14.546526365980652
13	23.918665585138235	34.12448814431885	26.1163040411105	15.840542229432412
14	16.49616451649253	36.02448898247235	30.412399456504026	17.066947044531094
15	15.51189293241037	24.19888823595697	44.0193557579748	16.269863073657863
16	20.580765867409706	20.416487782981573	42.00881178704569	16.993934562563037
17	21.99575521820395	20.146974871227027	28.221652484799154	29.635617425769865
18	24.715004530685263	23.85310335643222	31.824408705993452	19.607483406889063
19	28.97207924461882	25.961897542254576	25.732895382612416	19.333127830514186
20	30.314428626108107	25.226278160793232	26.14042423604637	18.31886897705229
21	25.247418254424286	25.71864677324875	30.279878076605364	18.7540568957216
22	22.905431141499864	22.47564465644554	30.033647206294717	24.585276995759877
23	20.655454656361723	26.659800016576813	29.281730395822642	23.40301493123882
24	21.373007173662607	24.2079216680372	35.836370087605665	18.582701070694522
25	20.56102269626528	24.717984631990085	33.095328784332864	21.625663887411772
26	18.72006511521351	32.01373826701521	27.589871008177585	21.67632560959369
27	18.563051027715876	30.374682549364913	32.26090041898362	18.801366003935595
28	16.525872401374944	27.27025514323578	37.05821162258135	19.145660832807934
29	17.379671425205512	25.86085348238807	36.29027676759587	20.469198324810552
30	21.09874472545351	26.724989732619726	33.03675116806002	19.13951437386675
31	29.112889031271504	25.08621339946675	27.217917114069895	18.58298045519185
32	29.43092171739513	26.456594358854485	26.775837711133192	17.33664621261719
33	27.000462846983904	26.21604430665615	26.44979600275287	20.333696843607076
34	20.526006505933662	26.712510558405793	29.79672915256163	22.964753783098914
35	20.60153344837766	26.413569146266163	30.68526498222649	22.299632423129683
36	28.598355915361395	25.34166395818918	28.145659901526276	17.91432022492315
37	21.147450756154143	30.837250148772245	28.267937183189623	19.747361911883992
38	21.34581374925614	31.677359332233802	25.201506068696922	21.775320849813138
39	20.995279333276837	29.59398913566818	26.959765838539973	22.45096569251501
40	24.401721381016195	25.854334510783776	27.091169680449322	22.652774427750703
41	18.91414421268983	24.327684489224605	29.210766733501647	27.547404564583918
42	22.124923984134686	24.533218351091325	27.69305701585693	25.64880064891706
43	22.434668263504282	24.767715072514246	27.783484464825026	25.014132199156446
44	20.208905101467792	28.65125271352193	29.36116872122922	21.778673463781058
45	19.359203716931354	33.067110950102865	24.657451324235954	22.916234008729834
46	22.84042768178851	30.08486769747129	26.751251875368435	20.32345274537176
47	22.20901871783004	27.186905434866627	28.183283680499617	22.42079216680372
48	23.146446834527083	25.164720443215565	30.693553388980515	20.995279333276837
49	21.836133542064594	24.48954124134257	31.21106660619544	22.46325861039739
50	19.623501451402465	30.741141881691842	28.629553850896215	21.005802816009478
51	18.65021899088182	31.85057772057639	26.601315528469744	22.897887760072045
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	812.0
1	1831.0
2	2850.0
3	6391.0
4	9932.0
5	7205.0
6	4478.0
7	4602.0
8	4726.0
9	4835.0
10	4944.0
11	5002.5
12	5061.0
13	4957.0
14	4853.0
15	4593.0
16	4333.0
17	4234.0
18	4135.0
19	4144.5
20	4154.0
21	4375.5
22	4597.0
23	5058.0
24	5519.0
25	6470.0
26	9252.0
27	11083.0
28	11628.5
29	12174.0
30	15712.5
31	19251.0
32	19717.0
33	20183.0
34	23089.5
35	25996.0
36	29731.0
37	33466.0
38	38472.0
39	43478.0
40	55026.0
41	66574.0
42	79925.5
43	93277.0
44	94427.5
45	95578.0
46	93314.5
47	91051.0
48	87842.5
49	84634.0
50	78392.5
51	72151.0
52	68771.5
53	65392.0
54	60228.0
55	55064.0
56	51418.0
57	47772.0
58	45566.5
59	43361.0
60	39618.0
61	35875.0
62	31696.0
63	27517.0
64	24744.0
65	21971.0
66	18083.0
67	14195.0
68	12120.0
69	10045.0
70	8485.0
71	6925.0
72	5394.0
73	3863.0
74	3146.0
75	2001.0
76	1573.0
77	1079.5
78	586.0
79	463.5
80	341.0
81	220.5
82	100.0
83	61.5
84	23.0
85	17.0
86	11.0
87	21.5
88	32.0
89	16.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1073789.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.53324997020328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.33524716668079	17.514122086284054
2	6.219120694738817	2.6783576102935047
3	1.9578258194020677	1.2647505830190833
4	1.0590171094973566	0.9121632056611487
5	0.6985283195191536	0.7520792457735981
6	0.5188676125537991	0.6703743601538125
7	0.4146658614250793	0.6250372553723132
8	0.34120364735653835	0.5877778743418745
9	0.3008581300434137	0.5830607987813479
>10	4.642633349628525	25.335558658219643
>50	1.794556953471119	27.575435266112784
>100	0.7131322646440704	20.43842331986714
>500	0.0034744568313962018	0.4673216103291135
>1k	4.343071039245252E-4	0.10829666294999268
>5k	4.343071039245252E-4	0.4872414628406406
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5210	0.4851977436907996	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1158	0.10784241596812781	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.312816577558534E-5	0.0	0.0	0.01694932617115653	0.0
2	9.312816577558534E-5	0.0	0.0	0.06910109900548432	0.0
3	9.312816577558534E-5	0.0	0.0	0.11519954106439906	0.0
4	9.312816577558534E-5	0.0	0.0	0.22192441904321986	0.0
5	9.312816577558534E-5	0.0	0.0	0.43081089487785773	0.0
6	9.312816577558534E-5	0.0	0.0	0.61492527861619	0.0
7	9.312816577558534E-5	0.0	0.0	0.734408715306266	0.0
8	9.312816577558534E-5	0.0	0.0	0.9619208242960209	0.0
9	9.312816577558534E-5	0.0	0.0	1.0489025311304176	0.0
10	9.312816577558534E-5	0.0	0.0	1.1953931358954133	0.0
11	9.312816577558534E-5	0.0	0.0	1.425233449029558	0.0
12	9.312816577558534E-5	0.0	0.0	1.6174499831903661	0.0
13	9.312816577558534E-5	0.0	0.0	1.6917662594792833	0.0
14	9.312816577558534E-5	0.0	0.0	1.722126041522124	0.0
15	9.312816577558534E-5	0.0	0.0	1.7676657145863852	0.0
16	9.312816577558534E-5	0.0	0.0	1.8577206508913762	0.0
17	9.312816577558534E-5	0.0	0.0	1.9589509670894374	0.0
18	9.312816577558534E-5	0.0	0.0	2.091099834324993	0.0
19	9.312816577558534E-5	0.0	0.0	2.182737949448169	0.0
20	9.312816577558534E-5	0.0	0.0	2.2890903147638877	0.0
21	9.312816577558534E-5	0.0	0.0	2.426640615614427	0.0
22	9.312816577558534E-5	0.0	0.0	2.5590688673473094	0.0
23	9.312816577558534E-5	0.0	0.0	2.693918451390357	0.0
24	9.312816577558534E-5	0.0	0.0	2.798967022385217	0.0
25	9.312816577558534E-5	0.0	0.0	2.890325753011066	0.0
26	1.8625633155117067E-4	0.0	0.0	2.983640175118203	0.0
27	1.8625633155117067E-4	0.0	0.0	3.102937355476728	0.0
28	1.8625633155117067E-4	0.0	0.0	3.2118973094341627	0.0
29	1.8625633155117067E-4	0.0	0.0	3.346840021642986	0.0
30	1.8625633155117067E-4	0.0	0.0	3.4910955504293675	0.0
31	1.8625633155117067E-4	0.0	0.0	3.6246413401515567	0.0
32	1.8625633155117067E-4	0.0	0.0	3.742262213526121	0.0
33	1.8625633155117067E-4	0.0	0.0	3.8811163086975187	0.0
34	1.8625633155117067E-4	0.0	0.0	4.02853819512027	0.0
35	1.8625633155117067E-4	0.0	0.0	4.204364172104576	0.0
36	1.8625633155117067E-4	0.0	0.0	4.350296007874918	0.0
37	1.8625633155117067E-4	0.0	0.0	4.508707017859188	0.0
38	1.8625633155117067E-4	0.0	0.0	4.736871024009372	0.0
39	1.8625633155117067E-4	0.0	0.0	5.178112273454095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	30	2.1653668E-6	45.000004	1
CGCTAAC	30	2.1653668E-6	45.000004	35
TCGCTAA	30	2.1653668E-6	45.000004	34
ATACGTA	30	2.1653668E-6	45.000004	37
TCACGAC	175	0.0	45.0	25
AACGTAC	25	3.8906335E-5	45.0	37
CTATGCG	20	7.033079E-4	45.0	1
ATATGCG	55	1.8189894E-12	45.0	1
CGCTATC	20	7.033079E-4	45.0	41
ATATACG	35	1.211829E-7	45.0	1
CACGTAT	25	3.8906335E-5	45.0	42
CCCGGTC	20	7.033079E-4	45.0	44
TAATGCG	40	6.8139343E-9	45.0	1
CCGTTCG	20	7.033079E-4	45.0	32
AAGTCGC	20	7.033079E-4	45.0	40
CTCACGA	180	0.0	43.75	24
CGGGATA	155	0.0	43.548386	6
GGCGATA	220	0.0	41.93182	8
CATACGA	340	0.0	41.691177	18
ATAGCGG	135	0.0	41.666664	2
>>END_MODULE