FastQCFastQC Report
Thu 26 May 2016
SRR1547037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547037_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2470572
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125290.5071295230416276No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT207.0348626E-445.00000438
CAATACG207.0348626E-445.0000041
CGTTTTT79600.041.9472351
CGGTCTA4150.041.2048231
CGACGGT4450.040.4494428
AGGGCGA10200.039.2647066
CTACGCG750.039.0000041
GGCGATA3900.038.6538478
TAGGGAC22200.038.2094575
AGGGATC20650.038.0266386
TTAGGGA27600.037.7445684
GGGCGAT17900.037.5837977
ACGGTCT4550.037.58241730
TAAGGGA29500.037.525424
CGACTCA601.5643309E-1037.50000412
TCGACGA301.1403017E-437.50000425
TATCGAT301.1403017E-437.50000435
CGAGGGA14300.037.447554
TAGGGTA13300.037.3872155
CGTAAGG4100.037.3170742
Produced by FastQC (version 0.11.5)