##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547036_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1641203
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394497207231524	31.0	31.0	33.0	30.0	34.0
2	31.805778444226583	31.0	31.0	34.0	30.0	34.0
3	31.925875105029665	31.0	31.0	34.0	30.0	34.0
4	35.667970994447366	37.0	35.0	37.0	33.0	37.0
5	35.553547611112094	37.0	35.0	37.0	33.0	37.0
6	35.61079707994684	37.0	35.0	37.0	33.0	37.0
7	35.99238119842579	37.0	35.0	37.0	35.0	37.0
8	35.996216799506215	37.0	35.0	37.0	35.0	37.0
9	37.78969146412723	39.0	38.0	39.0	35.0	39.0
10	37.19426237948627	39.0	37.0	39.0	33.0	39.0
11	36.853972360518476	39.0	37.0	39.0	33.0	39.0
12	35.9653790542669	37.0	35.0	39.0	32.0	39.0
13	35.599466976358194	37.0	35.0	39.0	31.0	39.0
14	36.44921926172448	38.0	35.0	41.0	31.0	41.0
15	36.808424673852045	38.0	35.0	41.0	31.0	41.0
16	36.96745375191247	38.0	35.0	41.0	32.0	41.0
17	36.92926225457789	38.0	35.0	41.0	32.0	41.0
18	36.835591940789776	38.0	35.0	41.0	32.0	41.0
19	36.802839136901405	38.0	35.0	41.0	32.0	41.0
20	36.63415068093344	38.0	35.0	41.0	31.0	41.0
21	36.49369212705558	38.0	35.0	41.0	31.0	41.0
22	36.4114969324331	38.0	35.0	40.0	31.0	41.0
23	36.438250478460006	38.0	35.0	40.0	31.0	41.0
24	36.33324335868262	38.0	35.0	40.0	31.0	41.0
25	36.26852193177809	37.0	35.0	40.0	31.0	41.0
26	36.13888470835113	37.0	35.0	40.0	31.0	41.0
27	36.044857948712014	37.0	35.0	40.0	30.0	41.0
28	36.04176083031776	37.0	35.0	40.0	30.0	41.0
29	36.08362585249966	37.0	35.0	40.0	30.0	41.0
30	36.08289102566837	37.0	35.0	40.0	30.0	41.0
31	35.9622721869263	37.0	35.0	40.0	30.0	41.0
32	35.85836243292268	37.0	35.0	40.0	30.0	41.0
33	35.77143656208281	37.0	35.0	40.0	30.0	41.0
34	35.679822666665856	37.0	35.0	40.0	30.0	41.0
35	35.606020705543436	37.0	34.0	40.0	29.0	41.0
36	35.49618298284856	37.0	34.0	40.0	29.0	41.0
37	35.47578879638899	37.0	34.0	40.0	29.0	41.0
38	35.43199592006595	37.0	34.0	40.0	29.0	41.0
39	35.38925227409406	37.0	34.0	40.0	29.0	41.0
40	35.23113837837245	37.0	34.0	40.0	28.0	41.0
41	35.21887725040717	37.0	34.0	40.0	28.0	41.0
42	35.157769026744404	36.0	34.0	40.0	28.0	41.0
43	35.07692588911914	36.0	34.0	40.0	27.0	41.0
44	34.96051250210973	36.0	34.0	40.0	27.0	41.0
45	34.86171667977697	36.0	34.0	40.0	27.0	41.0
46	34.85467428465583	36.0	34.0	40.0	27.0	41.0
47	34.83388343794156	36.0	34.0	40.0	27.0	41.0
48	34.782019652657226	35.0	34.0	40.0	27.0	41.0
49	34.74364536257855	36.0	34.0	40.0	27.0	41.0
50	34.64188829779132	35.0	34.0	40.0	27.0	41.0
51	34.272246638593764	35.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	7.0
12	9.0
13	8.0
14	21.0
15	52.0
16	161.0
17	319.0
18	660.0
19	1329.0
20	2278.0
21	3692.0
22	5677.0
23	8213.0
24	11788.0
25	17001.0
26	22256.0
27	26779.0
28	29957.0
29	34540.0
30	40858.0
31	50724.0
32	63101.0
33	84059.0
34	153208.0
35	248406.0
36	105332.0
37	135666.0
38	203687.0
39	391101.0
40	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.29405503158354	23.66678588815643	30.135089930983554	13.904069149276477
2	30.837684308400604	25.150027144722497	30.665798198029126	13.346490348847768
3	28.966861503421576	24.70925290777558	32.578541472322435	13.74534411648041
4	27.201144526301746	27.001534849741315	31.205097724047544	14.592222899909396
5	24.389913983827714	30.791133089569055	30.017615127440056	14.801337799163175
6	22.143513020631815	40.43302382459696	26.77572487985947	10.647738274911758
7	87.08605821461452	4.3850760692004584	6.605825117307243	1.9230405988777743
8	88.65204365334452	2.7754640955445486	6.400548865679627	2.1719433854312964
9	84.99539666939434	4.363933041799217	7.828952299014808	2.8117179897916342
10	51.761360416718716	24.016102822137174	13.25034136544961	10.972195395694499
11	44.60124676837661	21.53542249191599	20.156251237659205	13.707079502048192
12	40.41925343787454	21.184338561408918	23.48052008191552	14.915887918801026
13	24.511227434997377	33.874176442524174	24.76451724740937	16.85007887506908
14	16.354771469464776	38.295567336886414	28.54704750113179	16.802613692517014
15	16.2572210750285	24.844824192985268	43.63945227982157	15.258502452164663
16	20.122129925426655	20.325456387783838	42.25010556280972	17.30230812397979
17	20.530488915752652	21.16526718510751	26.98496164094265	31.31928225819719
18	23.77999552767086	24.05168647632255	33.36540330477095	18.802914691235635
19	29.182435079633656	25.98209971587914	25.200417011180214	19.635048193306982
20	30.827265121986734	25.928846096430487	25.09683445618854	18.147054325394237
21	25.30704611190694	27.05545870925169	28.954492527737273	18.6830026511041
22	23.337332432368207	21.358844701112538	30.00890200663782	25.294920859881444
23	20.97016639623496	27.433535034971296	28.38612895540649	23.21016961338725
24	21.867800631609864	23.71748040918765	36.21867617838866	18.19604278081383
25	21.058394360721984	24.43414982790063	32.94479720059005	21.56265861078733
26	17.955426598659642	32.624666174751084	27.501290212118796	21.91861701447048
27	17.09946910894021	33.144833393553384	31.031261824405632	18.724435673100768
28	16.06090166786193	28.05423826303023	36.31287537251638	19.571984696591464
29	17.603063118943847	26.225335927365474	35.75322492098783	20.418376032702838
30	20.070277717016115	29.199008288432328	31.7718161616814	18.958897832870157
31	29.13838202830485	25.23618345810969	27.23843424609875	18.387000267486716
32	29.13917413019596	25.994529622478147	27.156299373081822	17.709996874244077
33	28.003726534743112	26.859017440255716	25.92232648855748	19.214929536443695
34	20.501607662184384	27.52584537074329	28.423662398862298	23.548884568210028
35	20.259468207162673	26.84122561316303	31.148371042460926	21.75093513721337
36	28.999764197360108	25.32605655729364	27.282974744745164	18.391204500601084
37	20.66904581578269	31.342923453101168	28.553384316260694	19.434646414855443
38	21.196646606178515	32.097491900758165	24.98983976997361	21.71602172308971
39	21.120970410119895	29.568371493349694	28.077635734275407	21.233022362255006
40	25.00342736395193	25.26878149747472	27.499827870165973	22.227963268407382
41	18.978152001915667	23.83318821620482	28.965581954212855	28.22307782766666
42	21.63809108318715	25.06222569663838	26.599756398203024	26.69992682197144
43	22.33258164894897	24.204135624904417	26.92555399910919	26.537728727037425
44	20.022142294402336	28.069470991705476	29.728802591757386	22.1795841221348
45	19.655033533328904	34.10754184582894	24.583796154406247	21.6536284664359
46	22.800774797511338	30.49318091668124	25.991909593146	20.71413469266142
47	21.32441873430648	27.322092391983198	27.521275552140718	23.832213321569604
48	22.20188483691536	25.52438668464535	31.489462302957037	20.78426617548225
49	22.456941645853682	24.299309713667352	31.49013254301875	21.753616097460217
50	20.21541515583386	30.707474943684606	28.507868922979057	20.569240977502478
51	18.9615178622023	31.99756520064855	26.917328325624556	22.123588611524596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1509.0
1	2581.5
2	3654.0
3	8772.0
4	13890.0
5	9733.0
6	5576.0
7	5682.0
8	5788.0
9	6122.5
10	6457.0
11	6366.5
12	6276.0
13	6001.0
14	5726.0
15	5452.0
16	5178.0
17	4757.5
18	4337.0
19	4915.0
20	5493.0
21	5621.5
22	5750.0
23	6374.0
24	6998.0
25	8670.5
26	12502.0
27	14661.0
28	16003.0
29	17345.0
30	22110.0
31	26875.0
32	30084.5
33	33294.0
34	35480.5
35	37667.0
36	44528.5
37	51390.0
38	59766.0
39	68142.0
40	86374.5
41	104607.0
42	126471.5
43	148336.0
44	154134.0
45	159932.0
46	155302.5
47	150673.0
48	146489.0
49	142305.0
50	129061.0
51	115817.0
52	108294.5
53	100772.0
54	91015.5
55	81259.0
56	75791.5
57	70324.0
58	64859.0
59	59394.0
60	54532.5
61	49671.0
62	45431.0
63	41191.0
64	35611.5
65	30032.0
66	24881.5
67	19731.0
68	15400.5
69	11070.0
70	9436.5
71	7803.0
72	6889.0
73	5975.0
74	4350.0
75	2284.5
76	1844.0
77	1339.0
78	834.0
79	537.5
80	241.0
81	225.5
82	210.0
83	140.5
84	71.0
85	43.5
86	16.0
87	14.5
88	13.0
89	8.0
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1641203.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.523739500576852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8116505816318	14.969339639857873
2	7.759649911057928	2.8747546713622283
3	2.455406469649258	1.3644992943544179
4	1.1348943692563074	0.8408995062710126
5	0.6975049162758963	0.6460199684733184
6	0.4748268762084386	0.5277341617654662
7	0.3549001251194661	0.4601854226504585
8	0.25938585004121056	0.3843836733039261
9	0.234375164543004	0.39073540440594984
>10	3.013917028373077	13.660304379504563
>50	1.3864864033138418	18.678359034389125
>100	1.407051445649765	43.398652457962676
>500	0.00796068710410041	0.8861833395407972
>1k	0.001658476480020919	0.42905208763872893
>5k	3.3169529600418374E-4	0.48889695851945664
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7957	0.48482728827573435	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1851	0.11278312311152246	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1753	0.10681189347082597	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2186182940196916E-4	0.0	0.0	0.053070826704557574	0.0
2	1.8279274410295374E-4	0.0	0.0	0.18565649709390003	0.0
3	1.8279274410295374E-4	0.0	0.0	0.26961929755185676	0.0
4	1.8279274410295374E-4	0.0	0.0	0.41305067075797447	0.0
5	1.8279274410295374E-4	0.0	0.0	0.7299523581177954	0.0
6	1.8279274410295374E-4	0.0	0.0	0.9902492257204014	0.0
7	1.8279274410295374E-4	0.0	0.0	1.1589669285274278	0.0
8	1.8279274410295374E-4	0.0	0.0	1.4886641079744554	0.0
9	1.8279274410295374E-4	0.0	0.0	1.5838991276520942	0.0
10	1.8279274410295374E-4	0.0	0.0	1.7674839736461607	0.0
11	1.8279274410295374E-4	0.0	0.0	2.0455117374267533	0.0
12	1.8279274410295374E-4	0.0	0.0	2.260293211747724	0.0
13	1.8279274410295374E-4	0.0	0.0	2.3502272418463774	0.0
14	1.8279274410295374E-4	0.0	0.0	2.3898932673167184	0.0
15	1.8279274410295374E-4	0.0	0.0	2.4502758037853942	0.0
16	1.8279274410295374E-4	0.0	0.0	2.5779260700839566	0.0
17	1.8279274410295374E-4	0.0	0.0	2.720199755910756	0.0
18	1.8279274410295374E-4	0.0	0.0	2.9123149299629603	0.0
19	1.8279274410295374E-4	0.0	0.0	3.0216249909365263	0.0
20	1.8279274410295374E-4	0.0	0.0	3.157805585293227	0.0
21	1.8279274410295374E-4	0.0	0.0	3.331580554020435	0.0
22	1.8279274410295374E-4	0.0	0.0	3.5041369044536235	0.0
23	1.8279274410295374E-4	0.0	0.0	3.6829691391010133	0.0
24	1.8279274410295374E-4	0.0	0.0	3.8239632757190916	0.0
25	2.4372365880393832E-4	0.0	0.0	3.9524665748234677	0.0
26	2.4372365880393832E-4	0.0	0.0	4.081822906733658	0.0
27	2.4372365880393832E-4	0.0	0.0	4.222573319692932	0.0
28	2.4372365880393832E-4	0.0	0.0	4.358814844964334	0.0
29	2.4372365880393832E-4	0.0	0.0	4.523693900145198	0.0
30	2.4372365880393832E-4	0.0	0.0	4.704841509551225	0.0
31	2.4372365880393832E-4	0.0	0.0	4.866491226252938	0.0
32	2.4372365880393832E-4	0.0	0.0	5.022839953375664	0.0
33	2.4372365880393832E-4	0.0	0.0	5.181504055257028	0.0
34	2.4372365880393832E-4	0.0	0.0	5.358813017036893	0.0
35	2.4372365880393832E-4	0.0	0.0	5.5633580976880985	0.0
36	2.4372365880393832E-4	0.0	0.0	5.7299432184805905	0.0
37	2.4372365880393832E-4	0.0	0.0	5.90755683483396	0.0
38	2.4372365880393832E-4	0.0	0.0	6.1265425422692985	0.0
39	2.4372365880393832E-4	0.0	0.0	6.4659886680684835	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACTG	45	3.8562575E-10	45.000004	44
CTGTCGA	25	3.891538E-5	45.0	11
TAACGCG	25	3.891538E-5	45.0	1
TATGCGA	20	7.034172E-4	45.0	15
CATTACG	70	0.0	45.0	30
ATATCGC	35	1.2123564E-7	45.0	38
GTATCGA	20	7.034172E-4	45.0	29
TTACGCA	20	7.034172E-4	45.0	1
CGCAATA	20	7.034172E-4	45.0	21
TGTACGA	20	7.034172E-4	45.0	12
ATCGATC	25	3.891538E-5	45.0	27
TACGTAC	35	1.2123564E-7	45.0	42
AATCGAC	20	7.034172E-4	45.0	29
ACGGGTA	120	0.0	44.999996	5
CCGAATT	30	2.1660726E-6	44.999996	36
AATGTCG	65	0.0	44.999996	1
TACGGGT	175	0.0	43.714287	4
TACGTAG	150	0.0	43.5	1
TATCGTG	120	0.0	43.124996	1
CGACGGT	355	0.0	43.09859	28
>>END_MODULE