FastQCFastQC Report
Thu 26 May 2016
SRR1547035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547035_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1460801
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63770.43654132219241365No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15910.1089128498679834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA700.045.0000049
TGCGATA207.033914E-445.00000420
ACAATCG207.033914E-445.00000432
ACGCTAG800.045.0000041
TACCGGA351.2122291E-745.00000429
TTGCGAT253.891327E-545.013
TACGTAG453.8562575E-1045.01
TCGTTAG800.042.1875041
AACCCGC1350.041.66666421
TCACGAC3250.041.5384625
CGTTAGG2300.041.0869562
CGGTCTA3300.040.9090931
GGTTACG501.0822987E-940.51
CGGGATT3900.040.3846176
CGTTTTT36250.040.1586231
ATAGGGA15950.039.921634
GGGCGAT10150.039.901487
ATTAGCG1300.039.807691
TATGCGA403.4590812E-739.37500410
ACTACGG1150.039.1304322
Produced by FastQC (version 0.11.5)