FastQCFastQC Report
Thu 26 May 2016
SRR1547034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547034_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1471058
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65920.44811285482965324No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT15810.1074736686112988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG850.045.01
CGTTGAC207.0339325E-445.027
CAACCCG207.0339325E-445.040
CGATTCG207.0339325E-445.010
TCGATAG850.045.01
GTCGTAC207.0339325E-445.020
TCGTAAT253.89134E-545.021
ACGTCGA207.0339325E-445.029
TATAGCG502.1827873E-1145.01
CCGAATA207.0339325E-445.026
TTCGCGA253.89134E-545.042
ATCGCAA351.2122473E-745.014
TAGTACG253.89134E-545.01
GCGTAAG650.044.9999961
CTACGGG2750.044.1818163
GCTAGCG1150.043.043481
CGTTTTT37550.042.723031
CGGTCTA4250.041.29411731
ACACGAC1150.041.0869626
CCAATCG501.0822987E-940.524
Produced by FastQC (version 0.11.5)