FastQCFastQC Report
Thu 26 May 2016
SRR1547033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547033_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1269774
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50990.401567523039533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT253.8910366E-545.00000445
ACGTATA253.8910366E-545.00000422
CTATCGA207.033566E-445.019
TAACGCG551.8189894E-1245.01
CTACGTA207.033566E-445.021
TCGTATA207.033566E-445.023
GCGATAT351.2120654E-745.09
GCTCGAT406.8157533E-945.032
TATCGAT207.033566E-445.011
CGATACA351.2120654E-745.010
TCACGAC2050.043.9024425
CACGACG2150.042.90697526
TATCGCG556.184564E-1140.9090921
CTAGACG501.0822987E-940.5000041
AGGGCGA5850.040.3846176
CGTTTTT34150.040.2562261
ACGGTCT2350.040.21276530
ATCGACA2100.039.64285718
CGGTCTA2450.039.48979631
GGGCGAT9500.039.0789457
Produced by FastQC (version 0.11.5)