##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547033_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1269774
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45325782383322	31.0	31.0	33.0	30.0	34.0
2	31.860568101095154	31.0	31.0	34.0	30.0	34.0
3	31.98327418895016	33.0	31.0	34.0	30.0	34.0
4	35.720624300072295	37.0	35.0	37.0	33.0	37.0
5	35.62774871748831	37.0	35.0	37.0	33.0	37.0
6	35.69477324311256	37.0	35.0	37.0	33.0	37.0
7	36.01539407800128	37.0	35.0	37.0	35.0	37.0
8	35.99857769965364	37.0	35.0	37.0	35.0	37.0
9	37.77186727716901	39.0	38.0	39.0	35.0	39.0
10	37.229202992028505	39.0	37.0	39.0	34.0	39.0
11	36.962212173189876	39.0	37.0	39.0	33.0	39.0
12	36.32631791169137	38.0	35.0	39.0	32.0	39.0
13	36.11432506887052	38.0	35.0	39.0	32.0	39.0
14	37.06090847662655	39.0	35.0	41.0	31.0	41.0
15	37.321173689176184	39.0	35.0	41.0	32.0	41.0
16	37.42942681138533	39.0	35.0	41.0	33.0	41.0
17	37.381434018967155	39.0	35.0	41.0	32.0	41.0
18	37.298151482074765	39.0	35.0	41.0	32.0	41.0
19	37.25857121030987	39.0	35.0	41.0	32.0	41.0
20	37.10250643027815	39.0	35.0	41.0	32.0	41.0
21	37.00093087431307	39.0	35.0	41.0	32.0	41.0
22	36.93447022856036	39.0	35.0	41.0	32.0	41.0
23	36.90237160313568	38.0	35.0	41.0	32.0	41.0
24	36.807967402073125	38.0	35.0	41.0	31.0	41.0
25	36.72310820665725	38.0	35.0	41.0	31.0	41.0
26	36.61266335584128	38.0	35.0	41.0	31.0	41.0
27	36.51699751294325	38.0	35.0	40.0	31.0	41.0
28	36.42788795486441	38.0	35.0	40.0	31.0	41.0
29	36.47817958156333	38.0	35.0	40.0	31.0	41.0
30	36.46134508975613	38.0	35.0	40.0	31.0	41.0
31	36.3531793846779	38.0	35.0	40.0	30.0	41.0
32	36.30375090370412	38.0	35.0	41.0	30.0	41.0
33	36.21983597081056	38.0	35.0	41.0	30.0	41.0
34	36.116480570558224	38.0	35.0	41.0	30.0	41.0
35	36.0326656554631	38.0	35.0	41.0	30.0	41.0
36	35.930790833644416	38.0	35.0	41.0	30.0	41.0
37	35.87822478645806	38.0	35.0	41.0	29.0	41.0
38	35.80517084142532	38.0	35.0	41.0	29.0	41.0
39	35.738696019921655	38.0	35.0	40.0	29.0	41.0
40	35.60820901987283	38.0	35.0	40.0	29.0	41.0
41	35.57151351342837	38.0	35.0	40.0	29.0	41.0
42	35.53576620721483	38.0	34.0	40.0	28.0	41.0
43	35.45936520987199	38.0	34.0	40.0	28.0	41.0
44	35.350561596000546	37.0	34.0	40.0	28.0	41.0
45	35.24173750604438	37.0	34.0	40.0	27.0	41.0
46	35.22748930124573	37.0	34.0	40.0	27.0	41.0
47	35.21412078054835	37.0	34.0	40.0	28.0	41.0
48	35.13789067975876	37.0	34.0	40.0	27.0	41.0
49	35.073279181964665	37.0	34.0	40.0	27.0	41.0
50	34.98165657825723	37.0	34.0	40.0	27.0	41.0
51	34.62683044384276	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	2.0
11	5.0
12	11.0
13	15.0
14	25.0
15	34.0
16	89.0
17	200.0
18	450.0
19	918.0
20	1553.0
21	2591.0
22	3939.0
23	5748.0
24	8334.0
25	12030.0
26	15890.0
27	19401.0
28	21178.0
29	24198.0
30	28721.0
31	35287.0
32	44758.0
33	59046.0
34	103329.0
35	155688.0
36	87029.0
37	115955.0
38	176113.0
39	346946.0
40	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.321639913874435	23.607508107742007	28.220454978602493	12.850396999781063
2	32.99421786869159	24.669350608848504	28.74511527248156	13.591316249978341
3	30.586230305550437	25.231419134428645	29.986123514893205	14.196227045127715
4	27.706741514631737	26.95400913863412	29.94934531656814	15.389904030165999
5	24.23439131688001	31.495604729660553	29.174640526581896	15.09536342687754
6	22.58110498403653	40.64172049514323	26.454786442311786	10.322388078508459
7	86.60344281738325	4.261782017902398	6.9137500059065635	2.221025158807788
8	87.16133737184727	3.7898082650928435	6.798375143923249	2.250479219136634
9	82.85080652147548	5.607690817421053	8.284387615433927	3.2571150456695444
10	45.372404853147096	29.64385788337137	13.948308911664595	11.035428351816938
11	38.298075090527924	23.45440999736961	22.994879403736412	15.252635508366055
12	34.65152066430719	22.742157265781156	26.22702937688124	16.379292693030415
13	24.958929699300818	30.15733508482612	27.38676331378655	17.496971902086514
14	17.998793486084924	33.591016984124735	30.563864120701794	17.846325409088546
15	17.254724069007555	26.707272317751034	39.44788600176095	16.59011761148047
16	22.006357036764022	23.65145293571927	36.78339610040842	17.558793927108287
17	22.155832455224317	23.609240699526058	27.6566538612383	26.57827298401133
18	23.869365729649527	25.859089885286675	31.341404060880123	18.93014032418367
19	27.356757974253686	26.527004018037854	26.565987333178974	19.550250674529483
20	28.173202475401133	26.845170872926992	26.43273527415115	18.548891377520725
21	25.81648387823345	25.915635380784295	29.757342645226633	18.51053809575562
22	24.15988987016587	22.416272502035795	29.440435857089533	23.983401770708802
23	21.501070269197513	27.039536169428573	29.511314611891564	21.94807894948235
24	20.750227993327947	25.6997701953261	34.33902410980222	19.210977701543737
25	20.997516093415047	26.49825874525703	32.13280473533086	20.371420425997066
26	20.17721263783949	31.31305255895931	26.78106497691715	21.728669826284047
27	19.521426647576657	30.07275310409569	31.227446773992853	19.178373474334805
28	18.07006601174697	27.681461425419013	34.929129120615166	19.31934344221885
29	19.416526090469645	26.866828270227614	33.75443189102943	19.962213748273314
30	21.48429563056103	28.153986457432584	31.053557562211857	19.30816034979453
31	27.358096795177723	25.55848521075404	27.83526832333943	19.248149670728807
32	27.125141954395033	26.64875796795335	27.574749522355944	18.651350555295668
33	25.981395114406187	26.89470724711642	27.006853188047636	20.11704445042976
34	21.196606640236766	26.983778215650972	29.431694143997277	22.38792100011498
35	21.33151253687664	27.636020268173706	29.830269008500725	21.20219818644893
36	27.269340843331175	26.534328156034064	27.6394066975698	18.556924303064957
37	21.911064488641287	31.00591128815049	27.188854079544868	19.894170143663363
38	22.201746137501637	31.343924194384197	25.34852658819601	21.105803079918157
39	22.274121221571715	28.681954426535743	26.805084999377844	22.2388393525147
40	23.798486974847492	25.595105900735092	28.307714601181	22.29869252323642
41	19.808249342008892	25.186765518903364	29.080923061899206	25.924062077188538
42	21.71843178392375	26.323503237584013	27.238232945390283	24.719832033101955
43	21.831444020747	25.115020468209302	27.880945743100742	25.172589767942956
44	20.92340841756092	28.19115842661765	30.07818714196385	20.807246013857583
45	20.350786832932474	31.662012295101334	26.24278021128169	21.7444206606845
46	22.29971632747245	29.221971783955254	27.795576220650286	20.68273566792201
47	21.491541014385238	27.745803583944863	28.96350059144383	21.79915481022607
48	22.18977550335729	26.81965452119826	29.68780271134863	21.302767264095817
49	21.676062039386537	26.398792226018173	30.413443652177474	21.511702082417816
50	20.84189784953858	29.953676796028265	28.761259877742024	20.443165476691128
51	20.391266477341638	31.10435400315332	26.58843227219962	21.915947247305425
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	730.0
1	1776.0
2	2822.0
3	6192.5
4	9563.0
5	6953.5
6	4344.0
7	4396.5
8	4449.0
9	4587.0
10	4725.0
11	4655.0
12	4585.0
13	4448.0
14	4311.0
15	4192.5
16	4074.0
17	4052.5
18	4031.0
19	4284.0
20	4537.0
21	5090.0
22	5643.0
23	6290.5
24	6938.0
25	8531.0
26	12142.0
27	14160.0
28	15956.5
29	17753.0
30	20663.0
31	23573.0
32	27181.5
33	30790.0
34	38479.0
35	46168.0
36	45316.0
37	44464.0
38	52729.0
39	60994.0
40	70607.5
41	80221.0
42	88584.0
43	96947.0
44	101371.5
45	105796.0
46	102024.5
47	98253.0
48	99938.5
49	101624.0
50	96597.0
51	91570.0
52	84580.5
53	77591.0
54	71082.0
55	64573.0
56	58988.5
57	53404.0
58	50085.0
59	46766.0
60	42160.5
61	37555.0
62	35310.0
63	33065.0
64	28958.5
65	24852.0
66	21423.0
67	17994.0
68	15153.5
69	12313.0
70	9986.5
71	7660.0
72	6311.0
73	4962.0
74	3935.5
75	2214.5
76	1520.0
77	1112.5
78	705.0
79	537.5
80	370.0
81	264.5
82	159.0
83	144.0
84	129.0
85	76.0
86	23.0
87	17.5
88	12.0
89	10.5
90	9.0
91	6.5
92	4.0
93	5.5
94	7.0
95	4.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1269774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.788037315687177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30306382901519	15.890400236125618
2	6.099864623927273	2.41408697597826
3	2.001125445189555	1.1879503494834611
4	1.068695385428087	0.8458953666381471
5	0.7417202754028875	0.7338594243737054
6	0.5437313228040704	0.6455625423212937
7	0.4043947654349121	0.5601525096086222
8	0.3878018114777666	0.6139069373290504
9	0.30329125341845276	0.5401384776149292
>10	5.392338416184128	27.519683511938727
>50	1.9555168515122119	27.22243661785919
>100	0.7936532772268418	20.80293447949335
>500	0.0036020572340098715	0.4275074629968367
>1k	8.004571631133048E-4	0.1916576621808716
>5k	4.002285815566524E-4	0.40382744605796045
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5099	0.401567523039533	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022996218224660452	0.0
2	0.0	0.0	0.0	0.06552347110588183	0.0
3	0.0	0.0	0.0	0.10009655261487477	0.0
4	0.0	0.0	0.0	0.16963648649287197	0.0
5	0.0	0.0	0.0	0.3191119049531649	0.0
6	0.0	0.0	0.0	0.4655946648773719	0.0
7	0.0	0.0	0.0	0.5553744209599504	0.0
8	0.0	0.0	0.0	0.737217804113173	0.0
9	0.0	0.0	0.0	0.8069940005071768	0.0
10	0.0	0.0	0.0	0.947806460047221	0.0
11	0.0	0.0	0.0	1.1300436140604548	0.0
12	0.0	0.0	0.0	1.295821146125216	0.0
13	0.0	0.0	0.0	1.3557530710189372	0.0
14	0.0	0.0	0.0	1.381505685263677	0.0
15	0.0	0.0	0.0	1.422457854704853	0.0
16	0.0	0.0	0.0	1.5034171435231782	0.0
17	0.0	0.0	0.0	1.5942994580137884	0.0
18	0.0	0.0	0.0	1.7169984579933122	0.0
19	0.0	0.0	0.0	1.7940987923835265	0.0
20	0.0	0.0	0.0	1.8834060234340915	0.0
21	0.0	0.0	0.0	1.9965757686013417	0.0
22	0.0	0.0	0.0	2.112738172304678	0.0
23	0.0	0.0	0.0	2.230081888588048	0.0
24	0.0	0.0	0.0	2.326634503462821	0.0
25	7.875417200226182E-5	0.0	0.0	2.414366651073341	0.0
26	7.875417200226182E-5	0.0	0.0	2.5009174861038264	0.0
27	7.875417200226182E-5	0.0	0.0	2.6061330598988484	0.0
28	7.875417200226182E-5	0.0	0.0	2.706544629201732	0.0
29	7.875417200226182E-5	0.0	0.0	2.8285348416332354	0.0
30	7.875417200226182E-5	0.0	0.0	2.9773014725455083	0.0
31	7.875417200226182E-5	0.0	0.0	3.0960627639249187	0.0
32	7.875417200226182E-5	0.0	0.0	3.204428504600031	0.0
33	1.5750834400452365E-4	0.0	0.0	3.325788683655517	0.0
34	1.5750834400452365E-4	0.0	0.0	3.4595132677153573	0.0
35	1.5750834400452365E-4	0.0	0.0	3.619541745223953	0.0
36	1.5750834400452365E-4	0.0	0.0	3.7492498665116782	0.0
37	1.5750834400452365E-4	0.0	0.0	3.8885659967836794	0.0
38	1.5750834400452365E-4	0.0	0.0	4.087577789433396	0.0
39	1.5750834400452365E-4	0.0	0.0	4.528758660990066	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCAT	25	3.8910366E-5	45.000004	45
ACGTATA	25	3.8910366E-5	45.000004	22
CTATCGA	20	7.033566E-4	45.0	19
TAACGCG	55	1.8189894E-12	45.0	1
CTACGTA	20	7.033566E-4	45.0	21
TCGTATA	20	7.033566E-4	45.0	23
GCGATAT	35	1.2120654E-7	45.0	9
GCTCGAT	40	6.8157533E-9	45.0	32
TATCGAT	20	7.033566E-4	45.0	11
CGATACA	35	1.2120654E-7	45.0	10
TCACGAC	205	0.0	43.90244	25
CACGACG	215	0.0	42.906975	26
TATCGCG	55	6.184564E-11	40.909092	1
CTAGACG	50	1.0822987E-9	40.500004	1
AGGGCGA	585	0.0	40.384617	6
CGTTTTT	3415	0.0	40.256226	1
ACGGTCT	235	0.0	40.212765	30
ATCGACA	210	0.0	39.642857	18
CGGTCTA	245	0.0	39.489796	31
GGGCGAT	950	0.0	39.078945	7
>>END_MODULE