FastQCFastQC Report
Thu 26 May 2016
SRR1547032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547032_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1295032
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141461.0923282204609615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA207.033619E-445.031
CGTGCGG551.8189894E-1245.02
CCGGTCA207.033619E-445.045
CGTTTTT81000.043.472221
CGGTCTA2750.043.36363631
TCTAGCG700.041.7857131
ACTACGG700.041.7857132
TCACGAC2900.041.1206925
CACGACG2900.041.1206926
ACGGGTA1150.041.086965
TATAGCG501.0822987E-940.51
CGACCCG1350.040.032
CGACGGT3000.039.7528
CGGGATA2500.039.66
ACGGTCT3300.039.54545230
TAGGGTA7400.039.5270275
ATAGACG403.458681E-739.3751
CGTTAGG2750.039.2727282
GGGCGAT11850.039.1139227
TTCGTAG750.039.01
Produced by FastQC (version 0.11.5)