FastQCFastQC Report
Thu 26 May 2016
SRR1547031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547031_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1345515
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69340.5153417093083318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG302.1657797E-645.00000416
GATCGAC207.033717E-445.011
CGAATCT351.2121382E-745.014
TTACGCG351.2121382E-745.01
CGACTAA253.8911614E-545.010
TACCGGT253.8911614E-545.040
TACGGGT1200.043.1250044
CGTTTTT37900.042.1503941
TACGGGA3450.041.7391324
CGTAAGG2650.041.6037752
TTAACGG650.041.538462
GTATCCG650.041.5384610
CGTTAGG3400.041.029412
CGGTCTA2150.040.81395331
TCATACG501.0822987E-940.51
TAATGCG501.0822987E-940.51
ATACCGG501.0822987E-940.52
ACGGGTA1900.040.2631575
TATAGCG950.040.2631571
AGGGTAC5700.039.868426
Produced by FastQC (version 0.11.5)