##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547031_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1345515
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.402652515951143	31.0	31.0	33.0	30.0	34.0
2	31.819345009160063	31.0	31.0	34.0	30.0	34.0
3	31.958780095353823	33.0	31.0	34.0	30.0	34.0
4	35.69490938413916	37.0	35.0	37.0	33.0	37.0
5	35.59564627670446	37.0	35.0	37.0	33.0	37.0
6	35.65746795836539	37.0	35.0	37.0	33.0	37.0
7	36.013475137772524	37.0	35.0	37.0	35.0	37.0
8	36.01245545385967	37.0	35.0	37.0	35.0	37.0
9	37.78767535107375	39.0	38.0	39.0	35.0	39.0
10	37.13327610617496	39.0	37.0	39.0	33.0	39.0
11	36.875044871294634	39.0	37.0	39.0	33.0	39.0
12	36.25762254601398	38.0	35.0	39.0	32.0	39.0
13	36.032503539536904	38.0	35.0	39.0	32.0	39.0
14	36.95302021902394	39.0	35.0	41.0	31.0	41.0
15	37.22323719913936	39.0	35.0	41.0	32.0	41.0
16	37.33106282724459	39.0	35.0	41.0	32.0	41.0
17	37.256983385543826	39.0	35.0	41.0	32.0	41.0
18	37.15973437679996	39.0	35.0	41.0	32.0	41.0
19	37.13060946923669	39.0	35.0	41.0	32.0	41.0
20	36.97171863561536	39.0	35.0	41.0	32.0	41.0
21	36.88129749575442	38.0	35.0	41.0	31.0	41.0
22	36.82157538191696	38.0	35.0	41.0	31.0	41.0
23	36.777142581093486	38.0	35.0	41.0	31.0	41.0
24	36.70819426019034	38.0	35.0	41.0	31.0	41.0
25	36.641713395985924	38.0	35.0	41.0	31.0	41.0
26	36.53120775316515	38.0	35.0	41.0	31.0	41.0
27	36.44947250680966	38.0	35.0	40.0	31.0	41.0
28	36.37727561565646	38.0	35.0	40.0	31.0	41.0
29	36.415597745101316	38.0	35.0	40.0	31.0	41.0
30	36.385230190670484	38.0	35.0	40.0	31.0	41.0
31	36.28364603887731	38.0	35.0	40.0	30.0	41.0
32	36.209542071251526	38.0	35.0	41.0	30.0	41.0
33	36.106921884928816	38.0	35.0	41.0	30.0	41.0
34	35.98846463993341	38.0	35.0	41.0	30.0	41.0
35	35.93321962222643	38.0	35.0	41.0	30.0	41.0
36	35.84309650951494	38.0	35.0	41.0	29.0	41.0
37	35.81783183390746	38.0	35.0	41.0	29.0	41.0
38	35.753681675789565	38.0	35.0	41.0	29.0	41.0
39	35.69269759162849	38.0	35.0	40.0	29.0	41.0
40	35.56895017892777	38.0	35.0	40.0	28.0	41.0
41	35.53448753822886	38.0	34.0	40.0	28.0	41.0
42	35.50346893197029	38.0	34.0	40.0	28.0	41.0
43	35.41488575006596	38.0	34.0	40.0	28.0	41.0
44	35.294302181692515	37.0	34.0	40.0	27.0	41.0
45	35.19166490154328	37.0	34.0	40.0	27.0	41.0
46	35.18270179076413	37.0	34.0	40.0	27.0	41.0
47	35.157485423796835	37.0	34.0	40.0	27.0	41.0
48	35.082268871027075	37.0	34.0	40.0	27.0	41.0
49	35.022751139898105	37.0	34.0	40.0	27.0	41.0
50	34.95131603884015	36.0	34.0	40.0	27.0	41.0
51	34.59981122469835	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	6.0
12	7.0
13	9.0
14	25.0
15	33.0
16	96.0
17	255.0
18	565.0
19	1016.0
20	1815.0
21	2758.0
22	4337.0
23	6388.0
24	9080.0
25	12988.0
26	17496.0
27	21403.0
28	23659.0
29	26540.0
30	31525.0
31	38725.0
32	48726.0
33	63781.0
34	110999.0
35	163555.0
36	91067.0
37	120675.0
38	184355.0
39	363353.0
40	271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.49848570993263	23.835111462897107	29.629472729772615	14.03693009739765
2	30.662608740891034	24.92190722511455	30.67769590082608	13.73778813316834
3	30.51879763510626	25.116999810481488	31.178990943987987	13.185211610424261
4	27.492075524984855	26.31884445732675	31.48497043882826	14.70410957886014
5	24.577280818125402	31.051158849957076	29.67183569116658	14.699724640750938
6	23.25771173119586	40.76335083592528	25.496185475449924	10.48275195742894
7	87.67995897481633	4.0500477512328	6.521443462168761	1.7485498117821057
8	89.08388237960929	2.9082544601881066	6.09402347799913	1.9138396822034685
9	84.53610699248986	4.989910926299595	7.871335510938191	2.6026465702723494
10	45.40930424409984	27.53012786925452	17.339531703474133	9.721036183171499
11	39.55474297945397	23.780559859979263	22.044644615630446	14.620052544936327
12	35.78674336592309	22.44099842811117	25.497225969238546	16.275032236727203
13	25.83523780857144	28.479875735313243	26.96372764331873	18.721158812796588
14	17.930680817382193	32.88606964619495	30.28944307569964	18.893806460723216
15	18.002177604857618	27.28531454498835	37.86022452369539	16.852283326458643
16	23.33002604950521	23.837266771459255	36.20227199250844	16.630435186527095
17	23.154033957258	24.31448181551302	27.74840860191079	24.783075625318187
18	24.899016361764826	26.37696346751987	30.791778612650173	17.932241558065126
19	27.734956503643588	28.489091537441052	25.891647436111825	17.884304522803536
20	29.157980401556284	27.284571335139336	26.545672103246716	17.011776160057675
21	26.480046673578517	26.80653876025165	29.358721381775748	17.35469318439408
22	24.67508723425603	22.04828634389063	30.8455126847341	22.431113737119244
23	23.001304333285024	25.432195107449562	30.082013206839015	21.484487352426395
24	22.385480652389607	25.04899610929644	33.80125825427439	18.76426498403957
25	21.199466375328406	26.198221498831302	31.643125494699053	20.959186631141236
26	20.457965908964226	31.602471915957832	27.336150098661104	20.603412076416834
27	18.888529670795197	31.01719415985701	30.548451708082037	19.54582446126576
28	17.493822068130047	29.188452005365974	33.83470269748015	19.48302322902383
29	20.030545924794595	27.398505404993628	32.92479087932873	19.646157790883045
30	21.93137943464027	27.6565478645723	31.88935091767836	18.52272178310907
31	27.317198247511175	25.847500771080217	27.73190934326262	19.10339163814599
32	27.73607131841711	26.603345187530426	27.926258718780538	17.734324775271922
33	26.496248648287086	27.219689115320158	26.89698739887701	19.387074837515748
34	20.898837991401063	27.905151559068457	29.507809277488544	21.688201172041932
35	20.685388122763403	29.372173480042957	29.430143848266276	20.51229454892736
36	25.987744469589714	28.41722314504112	27.164765907477808	18.430266477891365
37	22.190313746037763	30.89701712727097	27.48598120422292	19.42668792246835
38	21.926251286682053	32.144643500815675	24.848106487107167	21.08099872539511
39	22.3368747282639	29.221970769556638	27.00445554304486	21.436698959134606
40	23.679111715588455	26.197032363072875	27.510507129240473	22.613348792098193
41	19.241554349078235	25.390501034919716	29.14467694525888	26.223267670743173
42	21.19567600509842	26.239321003481937	26.9318439407959	25.63315905062374
43	21.820046599257534	25.360698319974134	28.21952932520262	24.599725755565714
44	20.855508857203375	27.32403577812213	29.7034964307347	22.11695893393979
45	21.197608350705863	31.643274136668857	25.85010200555178	21.3090155070735
46	22.240926336755816	29.418995700531024	27.180224672337356	21.159853290375803
47	21.421686120184464	27.92529254597682	27.998127111180477	22.65489422265824
48	21.86501079512306	26.828389129812745	30.31292850692857	20.99367156813562
49	21.988309309074964	25.91572743521997	30.242769497181378	21.853193758523688
50	19.707769887366545	29.139920402225172	29.201829782648282	21.950479927760004
51	18.998524728449702	30.569038620899804	27.88359847344697	22.548838177203525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1205.0
1	2420.5
2	3636.0
3	8292.5
4	12949.0
5	9175.0
6	5401.0
7	5371.5
8	5342.0
9	5418.0
10	5494.0
11	5330.5
12	5167.0
13	5093.0
14	5019.0
15	4880.5
16	4742.0
17	4668.0
18	4594.0
19	4485.5
20	4377.0
21	4824.5
22	5272.0
23	5722.0
24	6172.0
25	7627.5
26	10979.5
27	12876.0
28	15825.0
29	18774.0
30	22048.5
31	25323.0
32	30357.5
33	35392.0
34	40222.5
35	45053.0
36	44485.0
37	43917.0
38	53209.0
39	62501.0
40	71722.5
41	80944.0
42	95837.5
43	110731.0
44	109424.5
45	108118.0
46	107151.5
47	106185.0
48	105800.5
49	105416.0
50	97891.5
51	90367.0
52	84868.5
53	79370.0
54	74163.0
55	68956.0
56	66808.0
57	64660.0
58	60086.5
59	55513.0
60	51601.0
61	47689.0
62	42428.0
63	37167.0
64	31115.5
65	25064.0
66	20596.0
67	16128.0
68	13279.0
69	10430.0
70	8607.0
71	6784.0
72	5863.5
73	4943.0
74	3723.5
75	1918.5
76	1333.0
77	889.0
78	445.0
79	381.0
80	317.0
81	204.0
82	91.0
83	63.5
84	36.0
85	29.5
86	23.0
87	13.5
88	4.0
89	4.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1345515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.897590338581924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.02386872259028	15.922821571505608
2	6.297879847470898	2.5062526641317353
3	2.0914484053511537	1.2484435075187306
4	1.0241639771020055	0.8151358102363402
5	0.7217628055812408	0.718067031354054
6	0.5456812688405961	0.6514645405696663
7	0.43438444494008494	0.6050242614409075
8	0.3801534501977989	0.6051310094267447
9	0.339410889469052	0.6078112951598017
>10	5.492612276471523	27.360270025029426
>50	1.7945031446649087	25.399872433599473
>100	0.849988157778766	22.461091177608427
>500	0.002636206071976677	0.3272389519442188
>1k	0.0011298026022757187	0.2517799126477159
>5k	3.766008674252396E-4	0.5195958078271613
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6934	0.5153417093083318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030768887749300453	0.0
2	0.0	0.0	0.0	0.08888789794242354	0.0
3	0.0	0.0	0.0	0.12002839061623245	0.0
4	0.0	0.0	0.0	0.16893159868154572	0.0
5	0.0	0.0	0.0	0.2939394952861915	0.0
6	0.0	0.0	0.0	0.41054912059694615	0.0
7	0.0	0.0	0.0	0.4980992408111392	0.0
8	0.0	0.0	0.0	0.6843476289747792	0.0
9	0.0	0.0	0.0	0.7480407130355291	0.0
10	0.0	0.0	0.0	0.8653935481953007	0.0
11	0.0	0.0	0.0	1.0449530477177884	0.0
12	7.432098490169192E-5	0.0	0.0	1.1912910669892198	0.0
13	7.432098490169192E-5	0.0	0.0	1.2486668673333259	0.0
14	7.432098490169192E-5	0.0	0.0	1.2763142737167552	0.0
15	7.432098490169192E-5	0.0	0.0	1.3220216794312958	0.0
16	7.432098490169192E-5	0.0	0.0	1.4110582193435228	0.0
17	7.432098490169192E-5	0.0	0.0	1.5241004373789961	0.0
18	7.432098490169192E-5	0.0	0.0	1.6467300624667878	0.0
19	7.432098490169192E-5	0.0	0.0	1.7269224051757135	0.0
20	7.432098490169192E-5	0.0	0.0	1.8204182041820418	0.0
21	7.432098490169192E-5	0.0	0.0	1.9420816564661114	0.0
22	7.432098490169192E-5	0.0	0.0	2.0721433800440723	0.0
23	7.432098490169192E-5	0.0	0.0	2.2077791774896602	0.0
24	7.432098490169192E-5	0.0	0.0	2.3119028773369306	0.0
25	7.432098490169192E-5	0.0	0.0	2.410898429225984	0.0
26	7.432098490169192E-5	0.0	0.0	2.5043942282323126	0.0
27	7.432098490169192E-5	0.0	0.0	2.612828545203881	0.0
28	7.432098490169192E-5	0.0	0.0	2.727134219982683	0.0
29	7.432098490169192E-5	0.0	0.0	2.859871499017105	0.0
30	7.432098490169192E-5	0.0	0.0	3.0039798887414855	0.0
31	7.432098490169192E-5	0.0	0.0	3.130548526029067	0.0
32	7.432098490169192E-5	0.0	0.0	3.25763741021096	0.0
33	7.432098490169192E-5	0.0	0.0	3.386881602955002	0.0
34	7.432098490169192E-5	0.0	0.0	3.5293549310115457	0.0
35	7.432098490169192E-5	0.0	0.0	3.703266035681505	0.0
36	7.432098490169192E-5	0.0	0.0	3.8502729438170515	0.0
37	7.432098490169192E-5	0.0	0.0	4.025967752124651	0.0
38	7.432098490169192E-5	0.0	0.0	4.237485275154866	0.0
39	7.432098490169192E-5	0.0	0.0	4.573862052819924	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATG	30	2.1657797E-6	45.000004	16
GATCGAC	20	7.033717E-4	45.0	11
CGAATCT	35	1.2121382E-7	45.0	14
TTACGCG	35	1.2121382E-7	45.0	1
CGACTAA	25	3.8911614E-5	45.0	10
TACCGGT	25	3.8911614E-5	45.0	40
TACGGGT	120	0.0	43.125004	4
CGTTTTT	3790	0.0	42.150394	1
TACGGGA	345	0.0	41.739132	4
CGTAAGG	265	0.0	41.603775	2
TTAACGG	65	0.0	41.53846	2
GTATCCG	65	0.0	41.53846	10
CGTTAGG	340	0.0	41.02941	2
CGGTCTA	215	0.0	40.813953	31
TCATACG	50	1.0822987E-9	40.5	1
TAATGCG	50	1.0822987E-9	40.5	1
ATACCGG	50	1.0822987E-9	40.5	2
ACGGGTA	190	0.0	40.263157	5
TATAGCG	95	0.0	40.263157	1
AGGGTAC	570	0.0	39.86842	6
>>END_MODULE