##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547030_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1477436
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.487941271229346	31.0	31.0	33.0	30.0	34.0
2	31.8904196188532	31.0	31.0	34.0	30.0	34.0
3	31.946884332045517	33.0	31.0	34.0	30.0	34.0
4	35.72173549311104	37.0	35.0	37.0	33.0	37.0
5	35.6423080255253	37.0	35.0	37.0	33.0	37.0
6	35.7191851288313	37.0	35.0	37.0	33.0	37.0
7	36.09141986522597	37.0	35.0	37.0	35.0	37.0
8	36.101217920776264	37.0	35.0	37.0	35.0	37.0
9	37.88907539818984	39.0	38.0	39.0	35.0	39.0
10	37.311296056140506	39.0	37.0	39.0	34.0	39.0
11	36.92051635400789	39.0	37.0	39.0	33.0	39.0
12	36.36926201879472	38.0	35.0	39.0	32.0	39.0
13	36.16587317487864	38.0	35.0	39.0	32.0	39.0
14	37.240782680265	39.0	35.0	41.0	32.0	41.0
15	37.49264672039939	39.0	35.0	41.0	33.0	41.0
16	37.57477007464283	39.0	35.0	41.0	33.0	41.0
17	37.52375534371709	39.0	35.0	41.0	33.0	41.0
18	37.38342371513893	39.0	35.0	41.0	33.0	41.0
19	37.31352830173354	39.0	35.0	41.0	33.0	41.0
20	37.114739318657456	38.0	35.0	41.0	32.0	41.0
21	37.019667857017154	38.0	35.0	41.0	32.0	41.0
22	36.92595482985388	38.0	35.0	41.0	32.0	41.0
23	36.88888994176398	38.0	35.0	41.0	32.0	41.0
24	36.82141358407403	38.0	35.0	41.0	32.0	41.0
25	36.754132835533994	38.0	35.0	41.0	32.0	41.0
26	36.62502470496184	38.0	35.0	40.0	32.0	41.0
27	36.55427984697814	38.0	35.0	40.0	31.0	41.0
28	36.49622589404888	38.0	35.0	40.0	31.0	41.0
29	36.5376144888848	38.0	35.0	40.0	31.0	41.0
30	36.4924531417943	38.0	35.0	40.0	31.0	41.0
31	36.373415159776805	38.0	35.0	40.0	31.0	41.0
32	36.195264634136436	38.0	35.0	40.0	30.0	41.0
33	36.03267417336521	38.0	35.0	41.0	30.0	41.0
34	35.877384874877826	38.0	35.0	41.0	30.0	41.0
35	35.7592640222656	38.0	35.0	41.0	30.0	41.0
36	35.63484983444291	38.0	35.0	40.0	29.0	41.0
37	35.57959329541178	38.0	35.0	40.0	29.0	41.0
38	35.546201662880826	38.0	35.0	40.0	29.0	41.0
39	35.49627936506217	38.0	35.0	40.0	29.0	41.0
40	35.37225165760141	38.0	35.0	40.0	27.0	41.0
41	35.35362411637458	38.0	35.0	40.0	28.0	41.0
42	35.31996783617023	38.0	35.0	40.0	27.0	41.0
43	35.23494960187785	38.0	34.0	40.0	27.0	41.0
44	35.10133230813382	37.0	34.0	40.0	27.0	41.0
45	34.98648943169112	37.0	34.0	40.0	26.0	41.0
46	34.95904661860141	37.0	34.0	40.0	26.0	41.0
47	34.93506520756229	37.0	34.0	40.0	26.0	41.0
48	34.86933511840784	37.0	34.0	40.0	26.0	41.0
49	34.80760114143692	37.0	34.0	40.0	26.0	41.0
50	34.719605451606704	37.0	34.0	40.0	26.0	41.0
51	34.36880582306103	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	5.0
12	4.0
13	3.0
14	23.0
15	25.0
16	72.0
17	169.0
18	379.0
19	870.0
20	1557.0
21	2540.0
22	4057.0
23	6594.0
24	10255.0
25	16173.0
26	21866.0
27	24754.0
28	25767.0
29	27726.0
30	32368.0
31	39371.0
32	50440.0
33	66678.0
34	118311.0
35	200917.0
36	104293.0
37	129440.0
38	196698.0
39	395743.0
40	337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.32503607601277	24.380210039555013	29.649135394020455	15.645618490411767
2	31.015827419935615	26.671815225837193	30.26628564621412	12.046071708013072
3	28.296724866593202	24.725470341862525	34.997793474641206	11.980011316903068
4	25.998418882442287	27.348460440926036	33.48591749490333	13.167203181728345
5	23.580716863539266	31.45774165513768	31.93146775900953	13.03007372231352
6	22.079332031979725	39.743718171210126	28.167176107797566	10.009773689012587
7	85.91465214060034	3.9603069100793538	8.575329151313491	1.5497117980068171
8	87.49312999006386	2.8995503020096978	8.224992486984208	1.3823272209422268
9	82.73353295844964	5.7917229578810865	9.145641503252932	2.329102580416343
10	47.70237086411865	28.543368376024407	14.910425900005144	8.843834859851798
11	36.333756589117904	23.84191261076622	26.13568371151102	13.688647088604853
12	32.94646942405627	23.705527684447922	27.591990448317222	15.756012443178587
13	21.49636261739933	33.87930170917725	29.04179944173555	15.582536231687872
14	15.668563646750181	37.34889362381856	30.709553577955322	16.272989151475937
15	15.381715350106537	27.20341185675725	42.31303420249676	15.10183859063946
16	18.14724969474143	24.985650816685123	40.49129708494987	16.375802403623574
17	18.672957745716225	24.29316735208835	30.306964227215254	26.72691067498017
18	20.9965101703221	27.573241751250137	33.472042105377156	17.95820597305061
19	26.01107594508324	27.517469453837595	28.71528783649512	17.75616676458405
20	27.478144569375594	27.304059194442264	28.450707847920313	16.76708838826183
21	22.742237227196306	28.443330201782008	30.744885057626863	18.069547513394827
22	20.958945091360977	25.26755812096091	30.55800725039866	23.21548953727945
23	18.94640444662239	29.217779991823672	30.19129085794579	21.64452470360814
24	18.736513798228824	27.035688855557872	36.69397523818291	17.533822108030396
25	17.90595328663983	28.96152523696458	34.42389382687304	18.70862764952255
26	16.946385494870846	36.343976997988406	28.78466478412601	17.924972723014736
27	17.97336737428897	33.38790986546964	31.916712466732903	16.722010293508482
28	15.515934361962211	30.175926402226562	37.87785054648729	16.430288689323937
29	16.36849244231222	27.32558296941458	37.18780373566097	19.11812085261223
30	18.322553396559986	30.463248492658906	33.24333507508955	17.97086303569156
31	26.27545287917717	27.82868428818575	27.85095259625459	18.04491023638249
32	25.380388727498175	29.918182581174413	28.444751583148104	16.256677108179304
33	24.823613340950132	29.5180298842048	27.061747513936307	18.596609260908764
34	18.910328433854325	29.976865326146108	29.472477995662754	21.64032824433681
35	20.272891685325117	27.951599933939608	31.44792735522892	20.32758102550635
36	25.078649768924	28.633186141396312	29.110296486616	17.17786760306369
37	19.434276679328242	33.07371689873538	29.942752173359793	17.549254248576588
38	19.35833430348252	33.98475466957621	26.7378756169472	19.91903540999407
39	21.740840212367914	30.905771891303583	28.42295706886796	18.930430827460544
40	21.8533323947704	29.176153823245137	28.472231622892636	20.498282159091833
41	17.47080753413346	27.946252832610007	30.255726813208828	24.327212820047706
42	20.781475475079798	28.104770697343234	27.444031416589283	23.669722410987685
43	21.533386217744795	27.228116818596543	28.086360424410937	23.15213653924772
44	18.97767483667651	31.738972111143898	29.339680365173177	19.94367268700641
45	18.56229305364158	35.48634255561662	25.991311975611804	19.960052415129994
46	21.410335202337023	33.48009659978503	26.674725673396342	18.434842524481603
47	20.660048895518994	29.630928175569025	28.423701601964485	21.285321326947496
48	21.44255317996854	27.756667632303532	31.30565384896537	19.49512533876256
49	20.33766606472294	27.83707720672841	31.653756914005072	20.171499814543576
50	18.832626252507723	32.45514526517562	29.18691571073129	19.52531277158537
51	19.056663029735297	32.78950831034305	27.567826965093584	20.586001694828067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1761.0
1	2393.0
2	3025.0
3	13857.5
4	24690.0
5	16582.5
6	8475.0
7	8667.5
8	8860.0
9	9390.0
10	9920.0
11	10575.5
12	11231.0
13	11369.5
14	11508.0
15	11160.5
16	10813.0
17	10075.0
18	9337.0
19	8912.0
20	8487.0
21	8211.0
22	7935.0
23	8719.5
24	9504.0
25	11089.0
26	15707.5
27	18741.0
28	23745.5
29	28750.0
30	32712.5
31	36675.0
32	39911.5
33	43148.0
34	45755.5
35	48363.0
36	50982.5
37	53602.0
38	62817.5
39	72033.0
40	86100.5
41	100168.0
42	117968.0
43	135768.0
44	139813.5
45	143859.0
46	140449.5
47	137040.0
48	130588.5
49	124137.0
50	111271.0
51	98405.0
52	87468.5
53	76532.0
54	65595.5
55	54659.0
56	51031.0
57	47403.0
58	42109.0
59	36815.0
60	33610.5
61	30406.0
62	25720.5
63	21035.0
64	16588.5
65	12142.0
66	9765.0
67	7388.0
68	6115.5
69	4843.0
70	3875.0
71	2907.0
72	2565.5
73	2224.0
74	1733.0
75	886.0
76	530.0
77	356.0
78	182.0
79	141.0
80	100.0
81	96.0
82	92.0
83	52.0
84	12.0
85	10.5
86	9.0
87	6.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1477436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.986026012938986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9978327970177	18.158462396380536
2	7.679259752609435	3.530313288671918
3	2.7793136313023643	1.916561262816962
4	1.500449755668774	1.3795750845964154
5	0.9389951553571578	1.0791883533531654
6	0.6805548736835767	0.938595121783386
7	0.498906231964299	0.8027510138164125
8	0.3861746879081556	0.7101297137436369
9	0.34126872763519167	0.7059970665742595
>10	4.211733830180857	22.862269512618543
>50	1.2412042113328212	20.230837355161725
>100	0.7365933262171632	25.00603374061589
>500	0.005933091632801734	0.9186435458284139
>1k	0.0014832729082004336	0.5997913174812002
>5k	0.0	0.0
>10k+	2.9665458164008666E-4	1.1608512265575208
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17024	1.1522664941154812	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2057	0.13922768905049018	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2007	0.13584344770264162	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1758	0.11898992579035571	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1727	0.1168916961546896	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.76848269569714E-5	0.0	0.0	0.06524817318652043	0.0
2	6.76848269569714E-5	0.0	0.0	0.21307183526054596	0.0
3	6.76848269569714E-5	0.0	0.0	0.32705308385608584	0.0
4	6.76848269569714E-5	0.0	0.0	0.4650624460213505	0.0
5	6.76848269569714E-5	0.0	0.0	0.8344185467255434	0.0
6	6.76848269569714E-5	0.0	0.0	1.1449565328041282	0.0
7	6.76848269569714E-5	0.0	0.0	1.3453036205967637	0.0
8	6.76848269569714E-5	0.0	0.0	1.6879242146529527	0.0
9	6.76848269569714E-5	0.0	0.0	1.8220078568547131	0.0
10	6.76848269569714E-5	0.0	0.0	2.0612060353206503	0.0
11	6.76848269569714E-5	0.0	0.0	2.4817318652043134	0.0
12	6.76848269569714E-5	0.0	0.0	2.773724208696688	0.0
13	6.76848269569714E-5	0.0	0.0	2.9030699130114606	0.0
14	6.76848269569714E-5	0.0	0.0	2.95850378628922	0.0
15	6.76848269569714E-5	0.0	0.0	3.0385072517523604	0.0
16	6.76848269569714E-5	0.0	0.0	3.2171951949187645	0.0
17	6.76848269569714E-5	0.0	0.0	3.454971992018605	0.0
18	6.76848269569714E-5	0.0	0.0	3.7121066496281396	0.0
19	6.76848269569714E-5	0.0	0.0	3.8899146900441033	0.0
20	6.76848269569714E-5	0.0	0.0	4.057434636762608	0.0
21	6.76848269569714E-5	0.0	0.0	4.271995538216207	0.0
22	6.76848269569714E-5	0.0	0.0	4.515187121472605	0.0
23	6.76848269569714E-5	0.0	0.0	4.780308588663062	0.0
24	6.76848269569714E-5	0.0	0.0	4.9697584193156255	0.0
25	6.76848269569714E-5	0.0	0.0	5.139579650150666	0.0
26	6.76848269569714E-5	0.0	0.0	5.2974206666143235	0.0
27	6.76848269569714E-5	0.0	0.0	5.457766021675389	0.0
28	6.76848269569714E-5	0.0	0.0	5.628805579395656	0.0
29	6.76848269569714E-5	0.0	0.0	5.819541421760401	0.0
30	6.76848269569714E-5	0.0	0.0	6.044931895527116	0.0
31	6.76848269569714E-5	0.0	0.0	6.247444897782374	0.0
32	6.76848269569714E-5	0.0	0.0	6.431344572624465	0.0
33	6.76848269569714E-5	0.0	0.0	6.616124150216997	0.0
34	6.76848269569714E-5	0.0	0.0	6.818163358683557	0.0
35	6.76848269569714E-5	0.0	0.0	7.059730506092988	0.0
36	6.76848269569714E-5	0.0	0.0	7.280992205415328	0.0
37	6.76848269569714E-5	0.0	0.0	7.501577056468097	0.0
38	6.76848269569714E-5	0.0	0.0	7.720740526154771	0.0
39	6.76848269569714E-5	0.0	0.0	7.942746758573637	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTC	30	2.1659234E-6	45.000004	44
TCGATAG	95	0.0	45.000004	1
CTTATCG	30	2.1659234E-6	45.000004	24
TTCGGCG	110	0.0	45.000004	33
GTTGCGA	30	2.1659234E-6	45.000004	9
GCGAACC	20	7.033942E-4	45.0	25
ATAATCG	20	7.033942E-4	45.0	1
TAGAACG	35	1.2122473E-7	45.0	1
TAGCGAT	40	6.8175723E-9	45.0	1
CCGGAAT	25	3.891348E-5	45.0	36
CCGTGCC	20	7.033942E-4	45.0	12
CCGTAAG	75	0.0	45.0	1
ACCGTGC	25	3.891348E-5	45.0	11
CGGTCTA	200	0.0	45.0	31
CGCAATA	20	7.033942E-4	45.0	41
TAGTCCG	25	3.891348E-5	45.0	11
TAGTACG	20	7.033942E-4	45.0	1
CGTTTTT	8015	0.0	43.905178	1
TTTAGCG	90	0.0	42.5	1
TATAGCG	80	0.0	42.1875	1
>>END_MODULE