FastQCFastQC Report
Thu 26 May 2016
SRR1547029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547029_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1266828
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53070.41892032698993076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC302.1656779E-645.00000434
CGCGAAT302.1656779E-645.00000417
CGTTACC302.1656779E-645.00000444
CCCGACA302.1656779E-645.00000410
CTTAACG253.891033E-545.01
CTCGTTA207.03356E-445.013
ACGCCGT502.1827873E-1145.039
CGTATAT207.03356E-445.031
CTCCGAT253.891033E-545.019
GGTCGAA253.891033E-545.027
ACGTTAC207.03356E-445.027
CGGCCTA207.03356E-445.045
TATATCG253.891033E-545.030
CGTTAAG207.03356E-445.015
CCCGTTA207.03356E-445.030
ACTCGAT207.03356E-445.010
ACCGTAT207.03356E-445.023
CGGTATG207.03356E-445.015
CGATACG253.891033E-545.010
TACGTGC207.03356E-445.017