FastQCFastQC Report
Thu 26 May 2016
SRR1547027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547027_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1089879
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.5349217665447265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTT750.045.0000045
TCGGACG351.2118471E-745.01
ACGTGCG253.890672E-545.01
ACGTCGA207.033127E-445.010
TCATACG207.033127E-445.01
TAGGACG253.890672E-545.01
CGTTTTT34400.042.7107541
ACGTAGG1950.041.538462
AGGGTAC7650.041.470596
CACGACG1100.040.90909226
TACGTAG501.0822987E-940.51
TAGGGCG1950.040.3846175
GGCGATT3400.040.367658
TACGGGC1400.040.178574
CGAGGGT2800.040.178574
CGGGTAT2750.040.0909086
AACGGGC900.040.04
AGGGCGA5250.039.8571436
CGTTAGG2150.039.767442
GCGGGAT4100.039.512195
Produced by FastQC (version 0.11.5)