##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547027_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1089879
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41862353527318	31.0	31.0	33.0	30.0	34.0
2	31.838427018045124	31.0	31.0	34.0	30.0	34.0
3	31.98660768764239	33.0	31.0	34.0	30.0	34.0
4	35.730188397060594	37.0	35.0	37.0	35.0	37.0
5	35.647028706856446	37.0	35.0	37.0	33.0	37.0
6	35.70225410343717	37.0	35.0	37.0	33.0	37.0
7	36.041997322638565	37.0	35.0	37.0	35.0	37.0
8	36.043970018690146	37.0	35.0	37.0	35.0	37.0
9	37.88133545099961	39.0	38.0	39.0	35.0	39.0
10	37.11060860884557	39.0	37.0	39.0	33.0	39.0
11	36.89410567595118	39.0	37.0	39.0	33.0	39.0
12	36.350397612946026	38.0	35.0	39.0	32.0	39.0
13	36.13747030633676	38.0	35.0	39.0	32.0	39.0
14	37.10339496402812	39.0	35.0	41.0	32.0	41.0
15	37.365500206903704	39.0	35.0	41.0	32.0	41.0
16	37.467209662724024	39.0	35.0	41.0	33.0	41.0
17	37.42573625145544	39.0	35.0	41.0	33.0	41.0
18	37.32087048195258	39.0	35.0	41.0	32.0	41.0
19	37.27519752192675	39.0	35.0	41.0	32.0	41.0
20	37.114168637068886	39.0	35.0	41.0	32.0	41.0
21	37.00841928324154	39.0	35.0	41.0	32.0	41.0
22	36.93878678275295	39.0	35.0	41.0	32.0	41.0
23	36.924630165366985	38.0	35.0	41.0	32.0	41.0
24	36.86033862474642	38.0	35.0	41.0	32.0	41.0
25	36.79315777256007	38.0	35.0	41.0	32.0	41.0
26	36.6956129992412	38.0	35.0	41.0	31.0	41.0
27	36.6142039620912	38.0	35.0	41.0	31.0	41.0
28	36.57131296226462	38.0	35.0	40.0	31.0	41.0
29	36.6271200747973	38.0	35.0	41.0	31.0	41.0
30	36.58549343550981	38.0	35.0	41.0	31.0	41.0
31	36.48922036299442	38.0	35.0	41.0	31.0	41.0
32	36.393409727134845	38.0	35.0	41.0	30.0	41.0
33	36.2897404207256	38.0	35.0	41.0	30.0	41.0
34	36.19460050152357	38.0	35.0	41.0	30.0	41.0
35	36.11729375462781	38.0	35.0	41.0	30.0	41.0
36	36.031045648186634	38.0	35.0	41.0	30.0	41.0
37	36.01680920542556	38.0	35.0	41.0	30.0	41.0
38	35.96147921007745	38.0	35.0	41.0	30.0	41.0
39	35.90654925913794	38.0	35.0	41.0	30.0	41.0
40	35.79590853663572	38.0	35.0	41.0	29.0	41.0
41	35.768744970771984	38.0	35.0	40.0	29.0	41.0
42	35.732069339807445	38.0	35.0	40.0	29.0	41.0
43	35.648270129069374	38.0	35.0	40.0	29.0	41.0
44	35.513389100991944	38.0	34.0	40.0	28.0	41.0
45	35.42125869018487	38.0	34.0	40.0	28.0	41.0
46	35.40213179628197	38.0	34.0	40.0	28.0	41.0
47	35.36672970118701	38.0	34.0	40.0	28.0	41.0
48	35.30551281380777	37.0	34.0	40.0	28.0	41.0
49	35.24009270753909	37.0	34.0	40.0	27.0	41.0
50	35.153499608672156	37.0	34.0	40.0	27.0	41.0
51	34.812638834219214	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	0.0
12	2.0
13	3.0
14	8.0
15	23.0
16	64.0
17	147.0
18	367.0
19	627.0
20	1179.0
21	1981.0
22	3067.0
23	4520.0
24	6733.0
25	9883.0
26	13694.0
27	16770.0
28	18348.0
29	20430.0
30	24115.0
31	29704.0
32	37272.0
33	48822.0
34	86165.0
35	136318.0
36	70777.0
37	94579.0
38	151065.0
39	313033.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.453621915827355	23.315248756972103	28.970738953590256	14.260390373610282
2	30.21473025904711	25.1005845603044	31.67250676451239	13.012178416136102
3	29.01413826672502	24.35830032508196	33.30773416131515	13.319827246877864
4	25.8613112097765	27.487730289325697	32.57756136231637	14.07339713858144
5	24.532172837535178	32.24403809964225	30.018653446850525	13.205135615972049
6	21.235476598778398	41.519838440781044	26.72461805393076	10.520066906509804
7	89.62802292731578	3.225403920985724	5.933686216543305	1.212886935155187
8	91.05643837526918	1.9733383247131104	5.679713069065465	1.2905102309522434
9	87.54503940345671	3.191271691628153	7.306774421747736	1.9569144831673977
10	43.30343093132357	21.981981486018174	23.200373619456837	11.514213963201419
11	38.30709647584732	24.35830032508196	21.97583401460162	15.3587691844691
12	35.4423747957342	22.773812505791927	24.61254873247397	17.171263965999895
13	25.314369760312843	30.378418154675884	25.88443304256711	18.422779042444162
14	17.37541506901225	33.67548140665156	29.94662710264167	19.00247642169452
15	17.986216818564262	27.017953369135473	39.1849920954528	15.810837716847468
16	22.08933285254602	24.13277070206876	37.36185393057394	16.416042514811277
17	22.56736757016146	23.626842979817024	28.289011899486088	25.516777550535423
18	23.977065343951026	25.886176355356877	31.598094834380696	18.538663466311398
19	26.706817912814174	27.071445545789945	27.494244773961146	18.727491767434735
20	28.47572987460076	27.417447257906613	27.305966992666157	16.80085587482647
21	25.766988812519553	26.18226426970333	30.75818508293122	17.292561834845888
22	23.791631915102503	22.0408871076514	31.116481737880996	23.0509992393651
23	20.759093440647998	25.890029994155316	31.190434901489066	22.160441663707623
24	21.747735299056135	25.168482005800648	34.74495792652212	18.338824768621105
25	20.676331959786364	27.078510550253743	32.70344689639859	19.541710593561305
26	19.612360638199284	32.83199327631783	27.226233370860438	20.32941271462245
27	18.122195216166197	32.82511177846348	30.726346686191768	18.32634631917855
28	16.952799347450497	29.61530591928095	35.46980903384688	17.962085699421678
29	18.254870494798045	27.173016454120138	34.77165813819699	19.800454912884824
30	20.940856737307538	28.720894704825028	31.709208086402253	18.62904047146518
31	26.3101683764895	26.094639863691288	29.217371836690127	18.37781992312908
32	26.87802957943038	26.654518529121123	28.744658810748717	17.722793080699784
33	25.955817113642894	27.510301602287964	27.185678410172137	19.348202873897012
34	20.37620690003202	27.791158468050124	29.758257568041955	22.074377063875897
35	20.10993880972108	28.66409940920047	30.418973115364185	20.806988665714268
36	25.7344163893423	27.67609982392541	27.855294028052658	18.734189758679634
37	20.940122710869737	31.515792120042686	28.748420696242427	18.79566447284515
38	21.506974627458643	30.433837150729577	26.47009438662457	21.58909383518721
39	21.187764880321577	29.582366482884797	28.199919440598453	21.029949196195176
40	23.50682965723718	26.88316776449496	27.822905111484854	21.78709746678301
41	19.05917996401435	26.043258013045484	29.364268877554295	25.533293145385866
42	21.593956760337615	26.77572464466239	27.857220847451874	23.77309774754812
43	21.72066807416236	25.978663686519326	28.198084374503956	24.10258386481435
44	20.072778721307593	28.385811635970597	29.84744178023432	21.693967862487487
45	20.127188431009312	32.08337806306938	26.85564177307756	20.933791732843737
46	22.196317205854964	30.30859388978043	27.661878061693084	19.833210842671527
47	21.12390458023322	28.40315301056356	28.129636409179366	22.343306000023855
48	21.241991083413847	27.334594023740248	30.769562492717085	20.653852400128823
49	22.1469539279131	25.886635121880502	30.97830126096567	20.988109689240734
50	20.529159659008016	29.922220723584914	28.708140995468305	20.840478621938765
51	19.384353675958526	31.462575203302386	27.935211156467826	21.217859964271263
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	865.0
1	1572.5
2	2280.0
3	6572.0
4	10864.0
5	7654.0
6	4444.0
7	4510.5
8	4577.0
9	4621.5
10	4666.0
11	4717.5
12	4769.0
13	4411.0
14	4053.0
15	3833.0
16	3613.0
17	3306.0
18	2999.0
19	3297.5
20	3596.0
21	4026.0
22	4456.0
23	4604.0
24	4752.0
25	6508.0
26	9725.5
27	11187.0
28	12949.0
29	14711.0
30	16766.5
31	18822.0
32	23582.5
33	28343.0
34	31589.5
35	34836.0
36	37991.5
37	41147.0
38	47023.0
39	52899.0
40	63814.5
41	74730.0
42	82219.5
43	89709.0
44	93591.0
45	97473.0
46	96597.0
47	95721.0
48	93415.0
49	91109.0
50	86083.5
51	81058.0
52	75680.5
53	70303.0
54	61929.0
55	53555.0
56	48082.5
57	42610.0
58	40071.0
59	37532.0
60	33519.0
61	29506.0
62	25697.0
63	21888.0
64	17961.5
65	14035.0
66	11379.5
67	8724.0
68	7440.5
69	6157.0
70	5188.0
71	4219.0
72	3244.0
73	2269.0
74	1956.0
75	1262.5
76	882.0
77	583.0
78	284.0
79	215.5
80	147.0
81	125.0
82	103.0
83	73.0
84	43.0
85	30.0
86	17.0
87	12.5
88	8.0
89	8.5
90	9.0
91	5.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1089879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.38376997682311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78486042144836	17.187346533761687
2	7.709099601831699	3.451174244316388
3	2.9431144632875155	1.9763399148506684
4	1.5829738341406607	1.4173168873093718
5	1.0139041384261975	1.1347498506540514
6	0.7777270738762873	1.0445078355836717
7	0.5685949960206402	0.8909109720629064
8	0.457944965958197	0.8200427824041897
9	0.3635506852261393	0.7323871421716494
>10	5.638371059704692	31.1494231688642
>50	1.7086864326288822	27.004651383048177
>100	0.44952420935958765	12.471403997197653
>500	0.0012360885683581698	0.1820575599780229
>1k	0.0	0.0
>5k	4.120295227860566E-4	0.5376877277973016
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5830	0.5349217665447265	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.175330472465291E-5	0.0	0.0	0.030829110387483382	0.0
2	9.175330472465291E-5	0.0	0.0	0.08826667914511611	0.0
3	9.175330472465291E-5	0.0	0.0	0.13001443279483318	0.0
4	9.175330472465291E-5	0.0	0.0	0.19121388704617667	0.0
5	9.175330472465291E-5	0.0	0.0	0.33389027589301196	0.0
6	9.175330472465291E-5	0.0	0.0	0.46197788928862743	0.0
7	9.175330472465291E-5	0.0	0.0	0.5411609912660029	0.0
8	9.175330472465291E-5	0.0	0.0	0.7176943495562351	0.0
9	9.175330472465291E-5	0.0	0.0	0.7749484117044185	0.0
10	9.175330472465291E-5	0.0	0.0	0.9015679722244396	0.0
11	9.175330472465291E-5	0.0	0.0	1.1010396566958351	0.0
12	9.175330472465291E-5	0.0	0.0	1.247477931036381	0.0
13	9.175330472465291E-5	0.0	0.0	1.310695957991667	0.0
14	9.175330472465291E-5	0.0	0.0	1.337396169666541	0.0
15	9.175330472465291E-5	0.0	0.0	1.3855666546469838	0.0
16	9.175330472465291E-5	0.0	0.0	1.488880875766943	0.0
17	9.175330472465291E-5	0.0	0.0	1.6089859516515137	0.0
18	9.175330472465291E-5	0.0	0.0	1.7407436972361152	0.0
19	9.175330472465291E-5	0.0	0.0	1.8249732309733466	0.0
20	9.175330472465291E-5	0.0	0.0	1.9202131612775364	0.0
21	9.175330472465291E-5	0.0	0.0	2.058026624973965	0.0
22	9.175330472465291E-5	0.0	0.0	2.1947390490136978	0.0
23	9.175330472465291E-5	0.0	0.0	2.3353968651565906	0.0
24	9.175330472465291E-5	0.0	0.0	2.4465101171781454	0.0
25	9.175330472465291E-5	0.0	0.0	2.558449148942222	0.0
26	9.175330472465291E-5	0.0	0.0	2.6567169383023255	0.0
27	9.175330472465291E-5	0.0	0.0	2.7712250625986923	0.0
28	9.175330472465291E-5	0.0	0.0	2.888669292646248	0.0
29	9.175330472465291E-5	0.0	0.0	3.027216782780474	0.0
30	9.175330472465291E-5	0.0	0.0	3.1766829161769334	0.0
31	9.175330472465291E-5	0.0	0.0	3.314863393092261	0.0
32	9.175330472465291E-5	0.0	0.0	3.4466211386768624	0.0
33	9.175330472465291E-5	0.0	0.0	3.573607712415782	0.0
34	9.175330472465291E-5	0.0	0.0	3.7207800131941253	0.0
35	9.175330472465291E-5	0.0	0.0	3.8962123318276616	0.0
36	9.175330472465291E-5	0.0	0.0	4.052468209773745	0.0
37	9.175330472465291E-5	0.0	0.0	4.20991688068125	0.0
38	9.175330472465291E-5	0.0	0.0	4.408654538714848	0.0
39	9.175330472465291E-5	0.0	0.0	4.729148832118061	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTT	75	0.0	45.000004	5
TCGGACG	35	1.2118471E-7	45.0	1
ACGTGCG	25	3.890672E-5	45.0	1
ACGTCGA	20	7.033127E-4	45.0	10
TCATACG	20	7.033127E-4	45.0	1
TAGGACG	25	3.890672E-5	45.0	1
CGTTTTT	3440	0.0	42.710754	1
ACGTAGG	195	0.0	41.53846	2
AGGGTAC	765	0.0	41.47059	6
CACGACG	110	0.0	40.909092	26
TACGTAG	50	1.0822987E-9	40.5	1
TAGGGCG	195	0.0	40.384617	5
GGCGATT	340	0.0	40.36765	8
TACGGGC	140	0.0	40.17857	4
CGAGGGT	280	0.0	40.17857	4
CGGGTAT	275	0.0	40.090908	6
AACGGGC	90	0.0	40.0	4
AGGGCGA	525	0.0	39.857143	6
CGTTAGG	215	0.0	39.76744	2
GCGGGAT	410	0.0	39.51219	5
>>END_MODULE